Objectif The present understanding of cellular signal transduction is restricted, at the best, to the wiring schemes of signalling pathways. Little is known about the details of their dynamic operation and the importance of quantitative, spatial and time-dependent parameters for signalling output. Those are, however, crucially important for drug discovery and application. QUASI is a multidisciplinary project with the goal to obtain a coherent and detailed picture of the dynamic operation of a model signalling transduction network. The signalling pathways contain the evolutionary conserved MAP kinase cascade module, which is of central importance for signalling in human cells and implicated in human diseases such as cancer and inflammatory disorders. MAP kinase pathways are currently being explored as drug targets. A better understanding of the dynamic operation of these pathways offers new opportunities for drug discovery and for efficient individualised treatment based on the genetic setup of the patient (pharmacogenomics). To achieve the goals of QUASI, quantitative data of high definition on signal transduction activation and deactivation will be obtained using frontline experimental approaches encompassing global gene expression, proteomics, bioimaging and chemical genetics. A software-implemented mathematical model of signalling dynamics will be constructed from pre-existing data and data generated within the consortium. Predictions from the model will be used as a basis for experimentation to further enhance the model, in a recursive manner. An interactive dynamic visual interface will be constructed to allow the experimenter to explore the effects of virtual manipulations of the system. This tool will enhance the intuitive understanding of intracellular signalling and this interface could be developed into a general tool for education as well as prediction of drug effects. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencescell biologycell signalingnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesmathematicsapplied mathematicsmathematical model Mots‑clés MAP kinases Signal transduction cellular dynamics mathematical models Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Appel à propositions FP6-2002-LIFESCIHEALTH Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur GOETEBORG UNIVERSITET Contribution de l’UE Aucune donnée Adresse Vasaparken 100 GOETEBORG Suède Voir sur la carte Coût total Aucune donnée Participants (5) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire UNIVERSITAT POMPEU FABRA Espagne Contribution de l’UE Aucune donnée Adresse ¨Placa de la Merce, 12 BARCELONA Voir sur la carte Coût total Aucune donnée INSTITUT FUER BIOCHEMIE UND MOLEKULARE ZELLBIOLOGIE DER UNIVERSITAET WIEN Autriche Contribution de l’UE Aucune donnée Adresse Dr. Bohrgasse 9 WIEN Voir sur la carte Coût total Aucune donnée EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Suisse Contribution de l’UE Aucune donnée Adresse Raemistrasse 101 ZURICH Voir sur la carte Coût total Aucune donnée MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Allemagne Contribution de l’UE Aucune donnée Adresse Hofgartenstrasse 8 101062 MUENCHEN Voir sur la carte Coût total Aucune donnée MAELARDALENS HOEGSKOLA Suède Contribution de l’UE Aucune donnée Adresse SANKTA URSULAS VAEG 2A 883 VAESTERAAS Voir sur la carte Coût total Aucune donnée