Obiettivo The genome sequences of microbial organisms responsible for diseases of world-wide medical importance have been sequenced or will be available in the near future. Technologies for producing large numbers of proteins have been developed and high-throughput assays such as protein micro arrays have been clinically validated for detecting the presence of antibodies, in serum, directed against microbial antigens. These achievements offer the opportunity of investigating the natural immune response against the w hole proteome of a variety of micro-organisms. Powerful combinations of genomic information, molecular tools and immunological assays are becoming available to help identify the antigens that function as targets of protective immunity or could be used as m arkers for serodiagnosis. We propose here to identify in micro-organisms of great medical relevance (M. pneumoniae, C. pneumoniae, L. pneumophila, coronavirus spp and P. falciparum), a large collection of surface and secreted proteins as well as putative e ndotoxins. This protein repertoire will be produced as recombinant molecules or as sets of overlapping synthetic peptides and printed on array slides. The serum reactivity of groups of individuals with proven historyof exposure to the selected micro-organi sms will be analysed against the arrayed proteins to identify diagnostic markers and correlates of protection. This project will significantly expand the SMEs bank of Intellectual Property and contribute to expertise within the RTDs. It is anticipated tha t the proposed work in high throughput protein expression, software analysis, surface peptides synthesis, protein and peptide surface capture, and array reader instrumentation will create an integrated platform of great commercial and research value. Fina lly it will contribute to unravelling how the humoral immune response interacts with the microbial proteomes thus filling the gap between genomic data and development of novel vaccines and diagnostic tools. Campo scientifico medical and health scienceshealth sciencespublic healthmedical and health scienceshealth sciencesinfectious diseasesRNA virusescoronaviruses Parole chiave Bioinformatics Geomics Micro-array Proteomics Serodiagnosis Vaccine Programma(i) FP6-SME - Horizontal research activities involving SMEs: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) SME-1 - Co-operative Research (all areas of science and technology) Invito a presentare proposte FP6-2002-SME-1 Vedi altri progetti per questo bando Meccanismo di finanziamento Cooperative - SMEs-Co-operative research contracts Coordinatore UNIVERSITA DEGLI STUDI DI PERUGIA Contributo UE Nessun dato Indirizzo Piazza dell'Universita 1 PERUGIA Italia Mostra sulla mappa Costo totale Nessun dato Partecipanti (8) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto DIAGNOSTIC MATRICES LTD Regno Unito Contributo UE Nessun dato Indirizzo 16 Bina Gardens LONDON Mostra sulla mappa Costo totale Nessun dato MIKURA LTD Regno Unito Contributo UE Nessun dato Indirizzo Unit 30, Huffwood Trading Estate PARTRIDGE GREEN WEST SUSSEX Mostra sulla mappa Costo totale Nessun dato CHELSEA INSTRUMENTS LTD Regno Unito Contributo UE Nessun dato Indirizzo Central Avenue 55 WEST MOLESEY Mostra sulla mappa Costo totale Nessun dato PROTEIN'EXPERT Francia Contributo UE Nessun dato Indirizzo 15, rue des Martyrs GRENOBLE Mostra sulla mappa Costo totale Nessun dato MICROTEST MATRICES LTD Regno Unito Contributo UE Nessun dato Indirizzo 14 Prices Gardens LONDON Mostra sulla mappa Costo totale Nessun dato INSTITUTO SUPERIORE DI SANITA Italia Contributo UE Nessun dato Indirizzo Viale Regina Elena 299 ROMA Mostra sulla mappa Costo totale Nessun dato BIOINFOBANK INSTITUTE Polonia Contributo UE Nessun dato Indirizzo Limanowskiego 24/a 16 POZNAN Mostra sulla mappa Costo totale Nessun dato DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES OEFFENTLICHEN RECHTS Germania Contributo UE Nessun dato Indirizzo Im Neuenheimer Feld 280 101949 HEIDELBERG Mostra sulla mappa Costo totale Nessun dato