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Boosting Lignocellulose Biomass Deconstruction with Designer Cellulosomes for Industrial Applications

Objective

The major bottleneck for plant biomass processing is fiber saccharification: the conversion of cell wall lignocellulosic biomass into fermentable sugars (en route to production of value-added chemicals like second generation biofuels). Some microbes enhance this step by using natural self-assembling proteinaceous nanocatalists known as cellulosomes. CellulosomePlus targets rational design of optimized cellulosomes to overcome this problem.This would allow efficient production of biofuels from low-value raw materials like inedible parts of plants and industrial residues (which are all renewable, sustainable and inexpensive). First we propose to characterize the physicochemical and structural properties (including mechanostability) as well as interactions of enzymes and scaffolds from natural cellulosomes and non-cellulosomal components. In parallel, we will characterize a suitable residual substrate from municipal waste (organic fraction of municipal solid waste) and develop improved assays to reliably follow cellulosomal enzymatic activity. The acquired knowledge will be complemented with rapid computational modelling at the atomic and supramolecular levels for testing and predictions. Experimental and theoretical knowledge will be then integrated to design improved cellulosomes (with high-selectivity, activity and cost-effectiveness). Further improvement will be obtained by iteration using high throughput screening of components. The improved cellulosomes generated through this innovative multidisciplinary approach represent a step towards green chemistry since they are biodegradable proteinaceous materials and therefore by-products and/or wastes are minimized due to the high enzymatic selectivity. Finally, the production of the optimized cellulosomes (and the process involved) will be scaled up to preindustrial scale to demonstrate their viable commercial production. These results will be patented and a roadmap will be drawn up towards future standardization.

Field of science

  • /engineering and technology/environmental engineering/energy and fuels/biofuels
  • /agricultural sciences/agricultural biotechnology/biomass
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-NMP-2013-SMALL-7
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Address
Calle Serrano 117
28006 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 1 174 727
Administrative Contact
Ana Maria De La Fuente (Ms.)

Participants (8)

WEIZMANN INSTITUTE OF SCIENCE
Israel
EU contribution
€ 513 290
Address
Herzl Street 234
7610001 Rehovot
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gaby Bernstein (Ms.)
LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN
Germany
EU contribution
€ 411 498,80
Address
Geschwister Scholl Platz 1
80539 Muenchen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Hermann E Gaub (Prof.)
INSTYTUT FIZYKI POLSKIEJ AKADEMII NAUK
Poland
EU contribution
€ 369 200
Address
Al Lotnikow 32/46
02 668 Warszawa
Activity type
Research Organisations
Administrative Contact
Marek Cieplak (Prof.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 454 266
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Emilie Floch (Ms.)
UNIVERSITY OF LIMERICK
Ireland
EU contribution
€ 347 479,20
Address
National Technological Park, Plassey
- Limerick
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sandra Hammersley (Ms.)
DESIGNER ENERGY LTD
Israel
EU contribution
€ 372 896,40
Address
Bergman 2
76705 Rehovot
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Alon Karpol (Mr.)
ABENGOA BIOENERGIA NUEVAS TECNOLOGIAS SA

Participation ended

Spain
EU contribution
€ 94 475,63
Address
Campus Palmas Altas Calle Energia Solar 1
41014 Sevilla
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Marina Aguiar (Ms.)
BIOPOLIS SL
Spain
EU contribution
€ 259 185,97
Address
Calle Catedratico Agustin Escardino 9
46980 Paterna Valencia
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Iryna Sukhotska (Ms.)