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Rewiring the Streptomyces cell factory for cost-effective production of biomolecules

Objective

STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.

Field of science

  • /engineering and technology/environmental engineering/energy and fuels/biofuels
  • /engineering and technology/other engineering and technologies/food and beverages
  • /engineering and technology/industrial biotechnology/bioprocessing technologies/fermentation
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/biochemistry/biomolecules
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /engineering and technology/industrial biotechnology/metabolic engineering

Call for proposal

FP7-KBBE-2013-7-single-stage
See other projects for this call

Funding Scheme

CP-TP - Collaborative Project targeted to a special group (such as SMEs)

Coordinator

KATHOLIEKE UNIVERSITEIT LEUVEN
Address
Oude Markt 13
3000 Leuven
Belgium
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 643 098,81
Administrative Contact
Myriam Witvrouw (Dr.)

Participants (16)

HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH
Germany
EU contribution
€ 594 794
Address
Inhoffenstrasse 7
38124 Braunschweig
Activity type
Research Organisations
Administrative Contact
Michael Straetz (Dr.)
NORGES TEKNISK-NATURVITENSKAPELIGE UNIVERSITET NTNU

Participation ended

Norway
EU contribution
€ 353 115,19
Address
Hogskoleringen 1
7491 Trondheim
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Stefan De Graaf (Dr.)
USTAV MOLEKULARNEJ BIOLOGIE SLOVENSKEJ AKADEMIE VIED
Slovakia
EU contribution
€ 183 680
Address
Dubravska Cesta 21
845 51 Bratislava
Activity type
Research Organisations
Administrative Contact
Jan Kormanec (Dr.)
TECHNISCHE UNIVERSITAT BERLIN
Germany
EU contribution
€ 777 550
Address
Strasse Des 17 Juni 135
10623 Berlin
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jacqueline Bock (Ms.)
UNIVERSITAET BIELEFELD
Germany
EU contribution
€ 578 500
Address
Universitaetsstrasse 25
33615 Bielefeld
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Iris Litty (Ms.)
THE UNIVERSITY OF MANCHESTER
United Kingdom
EU contribution
€ 320 223
Address
Oxford Road
M13 9PL Manchester
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Liz Fay (Ms.)
IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece
EU contribution
€ 251 170
Address
N Plastira Str 100
70013 Irakleio
Activity type
Research Organisations
Administrative Contact
Andreas Plagakis (Mr.)
TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel
EU contribution
€ 481 650
Address
Senate Building Technion City
32000 Haifa
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jack Lavan (Mr.)
FORSCHUNGSZENTRUM JULICH GMBH
Germany
EU contribution
€ 264 031
Address
Wilhelm Johnen Strasse
52428 Julich
Activity type
Research Organisations
Administrative Contact
Claudia Neuhs
PROGENUS SA
Belgium
EU contribution
€ 463 700
Address
Rue Des Praules 2
5030 Gembloux
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Robert Renaville (Prof.)
PHARMABIOTEC GMBH
Germany
EU contribution
€ 128 808
Address
Campus Gebaude C 2/2 Universitat Des Saarlandes
66123 Saarbruecken
Activity type
Research Organisations
Administrative Contact
Rudolf Guggenmoser (Mr.)
EntreChem SL
Spain
EU contribution
€ 596 450
Address
Edificio Cientifico Tecnologico Campus El Cristo
33006 Oviedo
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Francisco Moris (Dr.)
APRONEX S.R.O
Czechia
EU contribution
€ 306 450
Address
Nad Safinou Ii/365
25242 Vestec Jesenice U Prahy
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Ladislav Andera (Dr.)
MATIS OHF
Iceland
EU contribution
€ 280 290
Address
Vinlandsleid 12
113 Reykjavik
Activity type
Research Organisations
Administrative Contact
Kristinn Kolbeinsson (Mr.)
Q-BIOLOGICALS NV
Belgium
EU contribution
€ 1 042 070
Address
Technologiepark 4
9052 Gent
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Annie Van Broekhoven (Prof.)
UNIVERSITAT WIEN
Austria
EU contribution
€ 425 490
Address
Universitatsring 1
1010 Wien
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sergey Zotchev (Prof.)