Objective
Photoreceptor proteins are light sensing biomolecules used by bacteria, funghi and plants to react to light stimuli. To date, a range of light-signalling proteins and their function are known. However, the mechanisms of signal propagation from the light sensitive chromophore to the signalling cascade in the cell are still elusive.
Protein crystallography is an established method to gain structural information on static protein conformations. Only recently, time-resolved X-ray scattering methods for proteins in aqueous solution have been developed to study conformational changes that proteins undergo while functioning. Though, time-resolved X-ray solution scattering acts on proteins in their natural environment, the method lacks information content. To date, the method cannot be fully exploited because the scattering signal is averaged over all orientations that proteins adopt in solution.
I propose to establish a new and promising anisotropic X-ray scattering technique which should double the information content compared to the standard method. I plan to achieve this by arresting the protein rotation in hydrogels and by excitation with polarized light. Furthermore, I will develop indispensable analysis tools to derive structural changes from the anisotropic X-ray scattering.
With this new technique, I propose to elucidate the signal transduction sequence in the BLUF-domain containing photoreceptor proteins BlrP1 and AppA, covering all intermediates in the ns to ms time-scale. If successful, this project will uncover the sequence and nature of structural events that lead to signal transduction from the chromophores to the output domains of these proteins.
Photoreceptors are not only interesting as easily excitable targets for the development of time-resolved methods, they also reveal nature's highly optimized strategies to transform light signals into cellular response, ultimately providing guidelines to sensing applications.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
405 30 Goeteborg
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.