Single-molecule imaging of twin-arginine transporter assembly

Informations projet

SMTAT

N° de convention de subvention: 626436

Projet clôturé

Date de début 15 Juillet 2014

Date de fin 14 Juillet 2016

Financé au titre de

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Coût total € 221 606,40

Contribution de l’UE € 221 606,40

Coordonné par

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
Royaume-Uni

Objectif

The export of proteins across the cytoplasmic membrane is an essential function for all cells. In bacteria, two distinct transport mechanisms are used: The well-studied Sec pathway transports unfolded proteins via extrusion through a membrane-bound channel. In contrast, much less is known regarding the Twin Arginine Translocation (Tat) pathway responsible for the transport of folded proteins. The complexity required for this function is beyond that of a simple conformational change, and requires the orchestration of multiple copies of multiple proteins assembled into a large protein complex.
We recently developed a new form of artificial lipid bilayer that in addition to exceptional stability, and simple reconstitution of membrane proteins, is capable of single-molecule fluorescence imaging and single-channel electrical recording with gigaohm seals. Droplet Interface Bilayers (DIBs) are created by contacting aqueous droplets in a lipid/oil solution. We propose to reconstitute the minimal components of the Tat system in Droplet Interface Bilayers to create a working in vitro model of this important biological pathway. Using this method we will exploit single-molecule imaging to dissect the individual steps of Tat-driven transport, and in particular quantify the changes in stoichiometry of the assembled complex that occur during transport.

Champ scientifique (EuroSciVoc)

CORDIS classe les projets avec EuroSciVoc, une taxonomie multilingue des domaines scientifiques, grâce à un processus semi-automatique basé sur des techniques TLN. Voir: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• sciences naturelles › sciences biologiques › microbiologie › bactériologie
• sciences naturelles › sciences biologiques › biochimie › biomolécule › protéines
• sciences naturelles › sciences biologiques › biochimie › biomolécule › lipides

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Programme(s)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thème(s)

• FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Appel à propositions

FP7-PEOPLE-2013-IEF
Voir d’autres projets de cet appel

Régime de financement

MC-IEF -

Coordinateur

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

• Contribution de l’UE: € 221 606,40
• Adresse: WELLINGTON SQUARE UNIVERSITY OFFICES ; OX1 2JD Oxford ; Royaume-Uni
• Région: South East (England) › Berkshire, Buckinghamshire and Oxfordshire › Oxfordshire
• Type d’activité: Établissements d’enseignement supérieur ou secondaire
• Coût total: Aucune donnée