Single-molecule imaging of twin-arginine transporter assembly

Informazioni relative al progetto

SMTAT

ID dell’accordo di sovvenzione: 626436

Progetto chiuso

Data di avvio 15 Luglio 2014

Data di completamento 14 Luglio 2016

Finanziato da

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Costo totale € 221 606,40

Contributo UE € 221 606,40

Coordinato da

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

Obiettivo

The export of proteins across the cytoplasmic membrane is an essential function for all cells. In bacteria, two distinct transport mechanisms are used: The well-studied Sec pathway transports unfolded proteins via extrusion through a membrane-bound channel. In contrast, much less is known regarding the Twin Arginine Translocation (Tat) pathway responsible for the transport of folded proteins. The complexity required for this function is beyond that of a simple conformational change, and requires the orchestration of multiple copies of multiple proteins assembled into a large protein complex.
We recently developed a new form of artificial lipid bilayer that in addition to exceptional stability, and simple reconstitution of membrane proteins, is capable of single-molecule fluorescence imaging and single-channel electrical recording with gigaohm seals. Droplet Interface Bilayers (DIBs) are created by contacting aqueous droplets in a lipid/oil solution. We propose to reconstitute the minimal components of the Tat system in Droplet Interface Bilayers to create a working in vitro model of this important biological pathway. Using this method we will exploit single-molecule imaging to dissect the individual steps of Tat-driven transport, and in particular quantify the changes in stoichiometry of the assembled complex that occur during transport.

Campo scientifico (EuroSciVoc)

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• scienze naturali › scienze biologiche › microbiologia › batteriologia
• scienze naturali › scienze biologiche › biochimica › biomolecole › proteine
• scienze naturali › scienze biologiche › biochimica › biomolecole › lipidi

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Programma(i)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Argomento(i)

• FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Invito a presentare proposte

FP7-PEOPLE-2013-IEF
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Meccanismo di finanziamento

MC-IEF - Intra-European Fellowships (IEF)

Coordinatore

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

• Contributo UE: € 221 606,40
• Indirizzo: WELLINGTON SQUARE UNIVERSITY OFFICES ; OX1 2JD Oxford ; Regno Unito
• Regione: South East (England) › Berkshire, Buckinghamshire and Oxfordshire › Oxfordshire
• Tipo di attività: Higher or Secondary Education Establishments
• Costo totale: Nessun dato