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Regulation of cryptic splice sites in neuronal differentiation and disease

Objective

A recent era of RNA research discovered complex RNA regulatory networks that involve RNA binding proteins (RBPs) and RNA. These networks are particularly dynamic and complex in the central nervous system, and can lead to neurologic diseases if deregulated. The host lab studies the regulatory networks that control alternative splicing in the brain. Recently, the lab identified thousands of cryptic splice sites that are bound by the spliceosome, but do not lead to active splicing in the adult brain. In the present project, I will assess if splicing at some of these cryptic sites is regulated during brain development or disease. Moreover, I will determine the importance of such regulation for neuronal differentiation.
Most of the cryptic splice sites are present within long introns of genes that are only expressed in the brain. Therefore, I will employ genome-wide experimental and computational methods to study the regulation of cryptic splice sites in mouse embryonic stem cells (mESCs) and mouse brain from several developmental stages. It is known that binding of RBPs to target pre-mRNAs can actively repress cryptic splicing, which ensures expression of stable mRNAs. FUS and TDP-43 are two RBPs that regulate alternative splicing and lead to amyotrophic lateral sclerosis (ALS) when mutated. They have increased binding to the long introns and their depletion leads to decreased expression of long genes. I will therefore assess changes in splicing at cryptic sites upon depletion of FUS or TDP-43 in mESCs, in ALS mouse models, and in induced pluripotent stem cells (iPSCs) from ALS patients. Since de-repression of cryptic splice sites would lead to aberrant mRNAs, this may unravel the mechanism explaining how FUS and/or TDP-43 regulate the expression of long genes.
The study of genome-wide cryptic splicing regulation might uncover a novel mechanism controlling neuronal development, and explain how misregulation of long genes contributes to ALS neuropathology.

Field of science

  • /medical and health sciences/medical biotechnology/cells technologies/stem cells
  • /medical and health sciences/basic medicine/neurology/amyotrophic lateral sclerosis
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins

Call for proposal

FP7-PEOPLE-2013-IEF
See other projects for this call

Funding Scheme

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

University College London
Address
Gower Street
WC1E 6BT London
United Kingdom
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 231 283,20
Administrative Contact
Giles Machell (Mr.)