Periodic Reporting for period 3 - TREASURE (DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS) Période du rapport: 2018-04-01 au 2019-03-31 Résumé du contexte et des objectifs généraux du projet Research and development activities were carried out for the benefit of sustainable pork chains based on European local pig breeds and their production systems. Work program was planned to respond to consumer demands for quality and healthiness of pork products with regional identity and societal demands for environment preservation and development of local agro-economy. Description and evaluation of local pig breeds, with an emphasis on untapped ones, was performed using novel genomic tools. Performance of local pig breeds was evaluated in contrasted agro-geo-climatic conditions and production systems (indoor, outdoor, organic). Focus was on pig feeding and management and on the use of locally available feeding resources. Intrinsic quality of traditional and new regional high quality pork products and attitudes of consumers from various market areas was assessed; in particular the motives for the choice and willingness to pay for such products. Marketing strategies were addressed, esp. short chain distribution channels. All activities were driven from the perspective of sustainability (environmental impact, animal welfare, product quality, consumer acceptability and market potential). The activities engaged innovative approaches to answer socio-economic demands of regional pork chains involving partners from different sectors. The ambition was to enhance existing and create new networks between academia and non-academia partners, within and between regions and to tackle the value chain for regional high quality pork products, focusing on so far untapped pig breeds, their production systems and pork products. Cross-fertilizing interactions between research, local agriculture, businesses and end-users were achieved with partners from these complementary sectors in all research and development activities. Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent For genetic characterisation, candidate gene SNP data, SNP chip data and whole genome resequencing data in DNA pools for all breeds were produced. In addition RNAseq data and gut microbiota data was acquired from experiments with feeding and management. Data from high density SNP chip were used to determine the parameters of genetic diversity, linkage disequilibrium, effective size and selection signatures in all local pig breeds. Whole genome resequencing data on DNA pools have been acquired for all breeds and are being further mined. For several breeds, in relation to management factors studied in experiments, RNAseq data was acquired, analysed and functional interpretation of differential expression performed. Microbiota was also characterised in relation to feeding and management. The most remarkable result was found for Iberian “montanera” system, exhibiting a potential for certification of this traditional production system. Sixteen experiments were conducted to evaluate the impact of feeding and management practices esp. local feeding resources on performance and product quality. Environmental impact of the production systems practiced with local pig breeds was assessed in three case studies (for Gascon, Krškopolje and Mora Romagnola pigs), whereas the nutritional requirements of local pig breeds were assessed either in experiments (Iberian and Cinta Senese pigs) or by modelling with INRAPORC for several other breeds. Based on these results there is a potential to develop diets more adapted to nutritional requirements of local pig breeds thus contributing to economic and environmental sustainability. An open access book (chapter for each breed) was prepared representing a compendium of the available data on census and production system, performances and quality traits for each of the breeds studied in the project. The evaluation of intrinsic (sensory, technological, nutritional) qualities of traditional and innovative-traditional pork products have been undertaken using a quality toolbox developed in the project, in a variety of local breeds and production systems (connection with experiments). Quality of meat/pork products was also assessed using novel technologies (NIRS, biomarkers, CT). Results provide a first picture of the variability, between and within breeds, of the intrinsic qualities of pork from these genetic resources. Innovations aiming at improving the naturalness, healthiness, preservation or diversity of traditional pork products from local breeds were tested. Among them, reduced smoking duration and use of natural antioxidants to avoid nitrites addition are promising to improve health properties without impairing sensory, physical or chemical characteristics of pork products. Reduction of salt content and enrichment with omega3 by use of fish oil still needs process improvements before use at larger scale. Effects of feeding (feed supply level, use of local resources), rearing (extensive/intensive) and management practices (e.g. immunocastration) on the sensory, technological, and nutritional qualities of pork and pork products from local pig breeds were evaluated. A website and a database on carcass and meat quality for further implementation in the breeding programs were developed. Consumer studies highlighted that the information provided to consumers influence the acceptability of innovative and traditional pork products, this influence being either positive or negative; the most determining factor being sensory quality. Cost/benefit analysis was performed and the acquired data used to calculate farm net incomes of the different production systems as well as their social costs and benefits. Finally, focus groups with stakeholders demonstrated viable marketing strategies for the products from local pig breeds including trademark importance, which was one of focal points of the activities to maximise project’s impact (registration of joint EU trademark). The results obtained in scientific tasks were continuously disseminated at scientific events and project as a whole communicated to stakeholders and general public. Project management served to effectively implement the action. Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent) Research and development activities aimed to improve knowledge, skills and competences necessary to develop existing and create new sustainable pork chains based on European pig genetic resources (local pig breeds). Activities were focused on the genetic basis for their phenotypic singularity, adaptive traits, DNA markers that could be used in breeding programmes. Experiments were designed to improve knowledge about their nutritional requirements and to better exploit locally available feeding resources. The innovations to improve healthiness and quality attributes of products were tested with consumers and their motives to consume and purchase such products were identified. Multicriteria evaluation of production systems, costs and benefit analyses at product, farm, and society levels and development of new umbrella trademark along with marketing strategies are important for the sustainability of local pig breeds. The key innovation potential of TREASURE resides in the idea that sustainable pig production systems can be built on local pig breeds, locally available feeding resources and traditional products which comply with societal demands for biodiversity, environmental protection, local food chains, animal welfare and consumer demand for high quality and health benefits of the products. Logotype of the project TREASURE
