Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Completion of benchmarking protocol and flowsheets established for the case studies

Stakeholder analysis

Identification of genes predicted to encode relevant robust BVMOs and oxidases

Public website and newsletter, first press releases on the project scopes

Overall analysis of environmental benefits and techno-economics of the processes and platform

Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures

Project leaflet in several languages

Communication and dissemination plan

Quorum sensing technology and micro fluidics platform available

Optimized enzyme production processes for ROBOX enzymes in bioreactor including DSP

Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement

First generation production strains for robust BVMOs

First generation production strains for microbial and human P450s

96-well capillary electrophoreses screening system and first flow cytometer based screening system available

Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors

New vector and host systems for enzyme coexpression

Biocatalytic and structural exploration of P450-AX as well as generation of improved variants

Installation of advisory boards

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201700427

Author(s): Iwona Kaluzna, Jan Brummund, and Martin Schuermann
Published in: Chimica Oggi - Chemistry Today, Issue vol. 35(6) November/December 2017, 2017, Page(s) 55-58, ISSN 0392-839X

Published in: ISSN 1477-0520
DOI: 10.1039/C7OB02692G

Author(s): Elvira Romero, J. Rubén Gómez Castellanos, Giovanni Gadda, Marco W. Fraaije, Andrea Mattevi
Published in: Chemical Reviews, Issue 118/4, 2018, Page(s) 1742-1769, ISSN 0009-2665
DOI: 10.1021/acs.chemrev.7b00650

Author(s): Gonzalo de Gonzalo, Maximilian Fürst, Marco Fraaije
Published in: Catalysts, Issue 7/12, 2017, Page(s) 288, ISSN 2073-4344
DOI: 10.3390/catal7100288

Author(s): Friso S. Aalbers, Marco W. Fraaije
Published in: Applied Microbiology and Biotechnology, Issue 101/20, 2017, Page(s) 7557-7565, ISSN 0175-7598
DOI: 10.1007/s00253-017-8501-4

Published in: ISSN 0268-2575
DOI: 10.1002/jctb.5623

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201600148

Author(s): Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk, Manfred T. Reetz
Published in: Journal of the American Chemical Society, Issue 140/33, 2018, Page(s) 10464-10472, ISSN 0002-7863
DOI: 10.1021/jacs.8b04742

Published in: ISSN 1083-6160
DOI: 10.1021/acs.oprd.8b00079

Author(s): Marie A.F. Delgove, Daniela Valencia, Jordi Solé, Katrien V. Bernaerts, Stefaan M.A. De Wildeman, Marina Guillén, Gregorio Álvaro
Published in: Applied Catalysis A: General, Issue 572, 2018, Page(s) 134-141, ISSN 0926-860X
DOI: 10.1016/j.apcata.2018.12.036

Published in: ISSN 1864-5631
DOI: 10.1002/cssc.201900007

Author(s): Alexandra M. Weingartner, Daniel F. Sauer, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Published in: Applied Microbiology and Biotechnology, Issue 102/22, 2018, Page(s) 9657-9667, ISSN 0175-7598
DOI: 10.1007/s00253-018-9328-3

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201700577

Published in: ISSN 1083-6160
DOI: 10.1021/acs.oprd.8b00119

Author(s): Quoc-Thai Nguyen, Elvira Romero, Willem P. Dijkman, Suzan Pantaroto de Vasconcellos, Claudia Binda, Andrea Mattevi, Marco W. Fraaije
Published in: Biochemistry, Issue 57/43, 2018, Page(s) 6209-6218, ISSN 0006-2960
DOI: 10.1021/acs.biochem.8b00918

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201800394

Author(s): Maximilian JLJ Fürst, Filippo Fiorentini, Marco W Fraaije
Published in: Current Opinion in Structural Biology, Issue 59, 2019, Page(s) 29-37, ISSN 0959-440X
DOI: 10.1016/j.sbi.2019.01.019

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201800421

Author(s): Ángela Mourelle-Insua, Friso S. Aalbers, Iván Lavandera, Vicente Gotor-Fernández, Marco W. Fraaije
Published in: Tetrahedron, Issue 75/13, 2019, Page(s) 1832-1839, ISSN 0040-4020
DOI: 10.1016/j.tet.2019.02.015

Author(s): Maximilian J. L. J. Fürst, Elvira Romero, J. Rúben Gómez Castellanos, Marco W. Fraaije, Andrea Mattevi
Published in: ACS Catalysis, Issue 8/12, 2018, Page(s) 11648-11656, ISSN 2155-5435
DOI: 10.1021/acscatal.8b03793

Author(s): Joanne L. Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L. Galman, Nicholas J. Turner, Sabine L. Flitsch
Published in: Enzyme and Microbial Technology, Issue 113, 2018, Page(s) 1-8, ISSN 0141-0229
DOI: 10.1016/j.enzmictec.2018.02.005

Published in: ISSN 1439-4227
DOI: 10.1002/cbic.201700598

Published in: ISSN 1477-0520
DOI: 10.1039/c7ob02569f

Published in: ISSN 0730-2312
DOI: 10.1002/jcb.26474

Published in: ISSN 0268-2575
DOI: 10.1002/jctb.5770

Published in: ISSN 1867-3880
DOI: 10.1002/cctc.201700620

Published in: ISSN 0044-8249
DOI: 10.1002/ange.201608951

Author(s): Maximilian J. L. J. Fürst, Simone Savino, Hanna M. Dudek, J. Rúben Gómez Castellanos, Cora Gutiérrez de Souza, Stefano Rovida, Marco W. Fraaije, Andrea Mattevi
Published in: Journal of the American Chemical Society, Issue 139/2, 2017, Page(s) 627-630, ISSN 0002-7863
DOI: 10.1021/jacs.6b12246

Author(s): Marie A. F. Delgove, Juandré Luchies, Iris Wauters, Geert G. P. Deroover, Stefaan M. A. De Wildeman, Katrien V. Bernaerts
Published in: Polym. Chem., 2017, ISSN 1759-9954
DOI: 10.1039/C7PY00976C

Author(s): Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije
Published in: Methods in Enzymology, Issue 2018 June 9, 2018, Page(s) 151-187
DOI: 10.1016/bs.mie.2018.04.007

Author(s): Nguyen, Quoc-Thai
Published in: Issue 1, 2017