Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Rezultaty

Completion of benchmarking protocol and flowsheets established for the case studies
Stakeholder analysis
Identification of genes predicted to encode relevant robust BVMOs and oxidases
Public website and newsletter, first press releases on the project scopes
Overall analysis of environmental benefits and techno-economics of the processes and platform
Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures

Structural elucidation of relevant of BVMOs eg at least 3 new enzyme structures

Project leaflet in several languages
Communication and dissemination plan

Quorum sensing technology and micro fluidics platform available
Optimized enzyme production processes for ROBOX enzymes in bioreactor including DSP
Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement
First generation production strains for robust BVMOs
First generation production strains for microbial and human P450s
96-well capillary electrophoreses screening system and first flow cytometer based screening system available
Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors
New vector and host systems for enzyme coexpression
Biocatalytic and structural exploration of P450-AX as well as generation of improved variants

Installation of advisory boards

Publikacje

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters

Autorzy: Marie A. F. Delgove, Maximilian J. L. J. Fürst, Marco W. Fraaije, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Opublikowane w: ChemBioChem, 2017, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700427

Production of diclofenac metabolites by applying cytochrome P450 technology

Autorzy: Iwona Kaluzna, Jan Brummund, and Martin Schuermann
Opublikowane w: Chimica Oggi - Chemistry Today, Numer vol. 35(6) November/December 2017, 2017, Strona(/y) 55-58, ISSN 0392-839X
Wydawca: Teknoscienze s.r.l.

Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution

Autorzy: Guangyue Li, Maximilian J. L. J. Fürst, Hamid Reza Mansouri, Anna K. Ressmann, Adriana Ilie, Florian Rudroff, Marko D. Mihovilovic, Marco W. Fraaije, Manfred T. Reetz
Opublikowane w: Organic & Biomolecular Chemistry, Numer 15/46, 2017, Strona(/y) 9824-9829, ISSN 1477-0520
Wydawca: Royal Society of Chemistry
DOI: 10.1039/C7OB02692G

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes

Autorzy: Elvira Romero, J. Rubén Gómez Castellanos, Giovanni Gadda, Marco W. Fraaije, Andrea Mattevi
Opublikowane w: Chemical Reviews, Numer 118/4, 2018, Strona(/y) 1742-1769, ISSN 0009-2665
Wydawca: American Chemical Society
DOI: 10.1021/acs.chemrev.7b00650

Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides

Autorzy: Gonzalo de Gonzalo, Maximilian Fürst, Marco Fraaije
Opublikowane w: Catalysts, Numer 7/12, 2017, Strona(/y) 288, ISSN 2073-4344
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal7100288

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions

Autorzy: Friso S. Aalbers, Marco W. Fraaije
Opublikowane w: Applied Microbiology and Biotechnology, Numer 101/20, 2017, Strona(/y) 7557-7565, ISSN 0175-7598
Wydawca: Springer Verlag
DOI: 10.1007/s00253-017-8501-4

Application of a thermostable Baeyer-Villiger monooxygenase for the synthesis of branched polyester precursors

Autorzy: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M.A. De Wildeman
Opublikowane w: Journal of Chemical Technology & Biotechnology, 2018, ISSN 0268-2575
Wydawca: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5623

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst

Autorzy: Quoc-Thai Nguyen, Gonzalo de Gonzalo, Claudia Binda, Ana Rioz-Martínez, Andrea Mattevi, Marco W. Fraaije
Opublikowane w: ChemBioChem, Numer 17/14, 2016, Strona(/y) 1359-1366, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600148

Overriding Traditional Electronic Effects in Biocatalytic Baeyer–Villiger Reactions by Directed Evolution

Autorzy: Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk, Manfred T. Reetz
Opublikowane w: Journal of the American Chemical Society, Numer 140/33, 2018, Strona(/y) 10464-10472, ISSN 0002-7863
Wydawca: American Chemical Society
DOI: 10.1021/jacs.8b04742

Toward Upscaled Biocatalytic Preparation of Lactone Building Blocks for Polymer Applications

Autorzy: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Opublikowane w: Organic Process Research & Development, Numer 22/7, 2018, Strona(/y) 803-812, ISSN 1083-6160
Wydawca: American Chemical Society
DOI: 10.1021/acs.oprd.8b00079

High performing immobilized Baeyer-Villiger monooxygenase and glucose dehydrogenase for the synthesis of Ɛ-caprolactone derivative

Autorzy: Marie A.F. Delgove, Daniela Valencia, Jordi Solé, Katrien V. Bernaerts, Stefaan M.A. De Wildeman, Marina Guillén, Gregorio Álvaro
Opublikowane w: Applied Catalysis A: General, Numer 572, 2018, Strona(/y) 134-141, ISSN 0926-860X
Wydawca: Elsevier BV
DOI: 10.1016/j.apcata.2018.12.036

A prospective life-cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic and oxidative chemistry

Autorzy: Marie A. F. Delgove, Achille-B. Laurent, John M. Woodley, Stefaan M. A. De Wildeman, Katrien V. Bernaerts, Yvonne van der Meer
Opublikowane w: ChemSusChem, 2019, ISSN 1864-5631
Wydawca: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.201900007

A hydroquinone-specific screening system for directed P450 evolution

Autorzy: Alexandra M. Weingartner, Daniel F. Sauer, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Opublikowane w: Applied Microbiology and Biotechnology, Numer 102/22, 2018, Strona(/y) 9657-9667, ISSN 0175-7598
Wydawca: Springer Verlag
DOI: 10.1007/s00253-018-9328-3

Online Measurement of Oxygen-Dependent Enzyme Reaction Kinetics

Autorzy: Murray P. Meissner, Mathias Nordblad, John M. Woodley
Opublikowane w: ChemBioChem, Numer 19/1, 2018, Strona(/y) 106-113, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700577

Scoping Biocatalyst Performance Using Reaction Trajectory Analysis

Autorzy: Mathias Nordblad, Mafalda Dias Gomes, Murray P. Meissner, Hemalata Ramesh, John M. Woodley
Opublikowane w: Organic Process Research & Development, Numer 22/9, 2018, Strona(/y) 1101-1114, ISSN 1083-6160
Wydawca: American Chemical Society
DOI: 10.1021/acs.oprd.8b00119

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol

Autorzy: Quoc-Thai Nguyen, Elvira Romero, Willem P. Dijkman, Suzan Pantaroto de Vasconcellos, Claudia Binda, Andrea Mattevi, Marco W. Fraaije
Opublikowane w: Biochemistry, Numer 57/43, 2018, Strona(/y) 6209-6218, ISSN 0006-2960
Wydawca: American Chemical Society
DOI: 10.1021/acs.biochem.8b00918

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes

Autorzy: Friso S. Aalbers, Marco W. Fraaije
Opublikowane w: ChemBioChem, Numer 20/1, 2019, Strona(/y) 20-28, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800394

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases

Autorzy: Maximilian JLJ Fürst, Filippo Fiorentini, Marco W Fraaije
Opublikowane w: Current Opinion in Structural Biology, Numer 59, 2019, Strona(/y) 29-37, ISSN 0959-440X
Wydawca: Elsevier BV
DOI: 10.1016/j.sbi.2019.01.019

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations

Autorzy: Friso S. Aalbers, Marco W. Fraaije
Opublikowane w: ChemBioChem, Numer 20/1, 2019, Strona(/y) 51-56, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800421

What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis

Autorzy: Ángela Mourelle-Insua, Friso S. Aalbers, Iván Lavandera, Vicente Gotor-Fernández, Marco W. Fraaije
Opublikowane w: Tetrahedron, Numer 75/13, 2019, Strona(/y) 1832-1839, ISSN 0040-4020
Wydawca: Pergamon Press Ltd.
DOI: 10.1016/j.tet.2019.02.015

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme

Autorzy: Maximilian J. L. J. Fürst, Elvira Romero, J. Rúben Gómez Castellanos, Marco W. Fraaije, Andrea Mattevi
Opublikowane w: ACS Catalysis, Numer 8/12, 2018, Strona(/y) 11648-11656, ISSN 2155-5435
Wydawca: American Chemical Society
DOI: 10.1021/acscatal.8b03793

Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope

Autorzy: Joanne L. Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L. Galman, Nicholas J. Turner, Sabine L. Flitsch
Opublikowane w: Enzyme and Microbial Technology, Numer 113, 2018, Strona(/y) 1-8, ISSN 0141-0229
Wydawca: Elsevier BV
DOI: 10.1016/j.enzmictec.2018.02.005

Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus : P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids

Autorzy: Joanne L. Porter, Jack Manning, Selina Sabatini, Michele Tavanti, Nicholas J. Turner, Sabine L. Flitsch
Opublikowane w: ChemBioChem, Numer 19/5, 2018, Strona(/y) 513-520, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700598

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Autorzy: Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner
Opublikowane w: Organic & Biomolecular Chemistry, Numer 15/46, 2017, Strona(/y) 9790-9793, ISSN 1477-0520
Wydawca: Royal Society of Chemistry
DOI: 10.1039/c7ob02569f

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Autorzy: Astrid Weninger, Jasmin E. Fischer, Hana Raschmanová, Claudia Kniely, Thomas Vogl, Anton Glieder
Opublikowane w: Journal of Cellular Biochemistry, Numer 119/4, 2018, Strona(/y) 3183-3198, ISSN 0730-2312
Wydawca: John Wiley & Sons Inc.
DOI: 10.1002/jcb.26474

Co-immobilization of P450 BM3 and glucose dehydrogenase on different supports for application as a self-sufficient oxidative biocatalyst

Autorzy: Jordi Solé, Gloria Caminal, Martin Schürmann, Gregorio Álvaro, Marina Guillén
Opublikowane w: Journal of Chemical Technology & Biotechnology, Numer 94/1, 2019, Strona(/y) 244-255, ISSN 0268-2575
Wydawca: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5770

One-pot Biocatalytic Double Oxidation of α-Isophorone for the Synthesis of Ketoisophorone

Autorzy: Michele Tavanti, Fabio Parmeggiani, Jose Rubén Gómez Castellanos, Andrea Mattevi, Nicholas John Turner
Opublikowane w: ChemCatChem, 2017, ISSN 1867-3880
Wydawca: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201700620

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

Autorzy: Elvira Romero, J. Rubén Gómez Castellanos, Andrea Mattevi, Marco W. Fraaije
Opublikowane w: Angewandte Chemie, Numer 128/51, 2016, Strona(/y) 16084-16087, ISSN 0044-8249
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201608951

Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila : A Structurally Distinct Biocatalyst for Bulky Substrates

Autorzy: Maximilian J. L. J. Fürst, Simone Savino, Hanna M. Dudek, J. Rúben Gómez Castellanos, Cora Gutiérrez de Souza, Stefano Rovida, Marco W. Fraaije, Andrea Mattevi
Opublikowane w: Journal of the American Chemical Society, Numer 139/2, 2017, Strona(/y) 627-630, ISSN 0002-7863
Wydawca: American Chemical Society
DOI: 10.1021/jacs.6b12246

Increasing the solubility range of polyesters by tuning their microstructure with comonomers

Autorzy: Marie A. F. Delgove, Juandré Luchies, Iris Wauters, Geert G. P. Deroover, Stefaan M. A. De Wildeman, Katrien V. Bernaerts
Opublikowane w: Polym. Chem., 2017, ISSN 1759-9954
Wydawca: Royal Society of Chemistry
DOI: 10.1039/C7PY00976C

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins

Autorzy: Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije
Opublikowane w: Methods in Enzymology, Numer 2018 June 9, 2018, Strona(/y) 151-187, ISBN 978-0-12-815148-8
Wydawca: Elsevier
DOI: 10.1016/bs.mie.2018.04.007

Flavoenzymes at the crossroads of biocatalysis and structural biology

Autorzy: Nguyen, Quoc-Thai
Opublikowane w: Numer 1, 2017
Wydawca: Universityof Groningen

Prawa własności intelektualnej

KETOREDUCTASES

Numer wniosku/publikacji: WO 2019012095
Data: 2017-07-14
Wnioskodawca/wnioskodawcy: C-LECTA GMBH

KETOREDUCTASES

Numer wniosku/publikacji: WO 2019012095
Data: 2017-07-14
Wnioskodawca/wnioskodawcy: C-LECTA GMBH

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