Project description DEENESFRITPL Cell-based biosensors for advanced diagnosis Current diagnostic technologies for multiple biomarkers are complex and costly, but living cells offer a scalable and affordable solution. Funded by the European Research Council, the COMPUCELL project proposes to engineer chimeric receptors in the bacterium Bacillus subtilis to detect biomarkers, utilising sensing domains from antibodies. The key objective is to advance synthetic biology and explore biological computers capable of undertaking multiplexed biomarker analysis. COMPUCELL will deliver a prototype for the diagnosis of diabetic nephropathy in urine, but the reusable sensing platform has the potential for use in other applications including environmental monitoring. Show the project objective Hide the project objective Objective Early diagnostics based on multiple biomarkers is key in numerous diseases, yet current technologies for multiplexed detection are complicated and expensive. Living cells detect and process various environmental signals in parallel and can self-replicate, presenting an attractive platform for scalable and affordable autonomous diagnostic devices. In this project, I will apply my expertise in synthetic biology, the rational engineering of biological systems, to build cell-based biosensors for multiplexed diagnosis using the non-pathogenic bacterium Bacillus subtilis.In a first research line, I will conceive a scalable detection machinery by engineering chimeric receptors detecting extracellular biomarkers via sensing domains derived from antibodies. In a second research line, I will implement bio-molecular computing systems operating within and across bacterial cells to perform multiplexed biomarkers analysis. I will deploy in B. subtilis biomolecular logic gates and will engineer specific cell-cell communication systems to perform distributed multicellular computation in a bacterial consortia.My project is highly interdisciplinary and is at the cross-roads of genetic engineering, structural biology, biophysics, modeling, and clinics. On foundational point of view, I will make several breakthrough contributions to synthetic biology: (i) Advancing engineering frameworks for the Gram-positive model, B. subtilis. (ii) Pushing the limits of custom-ligand detection by engineered cells (iii) Exploring the frontiers of man-made biological computers. On an applied point of view, I plan to deliver a first prototype for the urinary diagnostic of diabetic nephropathy, a major complication of diabetes. Because of the modular design principles applied, my sensing platform will be reusable to diagnose other pathologies as well as for applications requiring custom-detection and bio-molecular computation like targeted therapy, drug delivery, or environmental monitoring. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiophysicsnatural sciencesbiological sciencesmolecular biologystructural biology Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-StG-2014 - ERC Starting Grant Call for proposal ERC-2014-STG See other projects for this call Funding Scheme ERC-STG - Starting Grant Coordinator INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Net EU contribution € 1 500 000,00 Address Rue de tolbiac 101 75654 Paris France See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE France Net EU contribution € 1 500 000,00 Address Rue de tolbiac 101 75654 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
