From Omics to Patient: Improving Diagnostics of Pathogenic Yeasts

Información del proyecto

OPATHY

Identificador del acuerdo de subvención: 642095

DOI

10.3030/642095

Proyecto cerrado

Fecha de la firma de la CE 18 Agosto 2015

Fecha de inicio 1 Septiembre 2015

Fecha de finalización 31 Agosto 2019

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 3 319 305,84

Aportación de la UE

€ 3 319 305,84

Coordinado por

FUNDACIO CENTRE DE REGULACIO GENOMICA
Spain

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Websites, patent fillings, videos etc. (17)

Full Map Transcriptomic/proteomics pipeline

Full Map Transcriptomicproteomics pipeline

Enrichment tool based on llama antibodies
Verification of gene expression data from in vitro, ex vivo and in vivo samples
Validated PCR assays for resistant Candida isolates
Comparative transcriptomics of all transcriptional data

Comparative transcriptomics of all transcriptional data; identification of Candida-specific, species-specific and stage specific fungal and host pattern and factors

OPATHY OMICS pipeline
Validated biomarkers candidiasis
Validated PCR assays for Candida pathogens
OPATHY final symposium and publication
Dissection of RNAseq samples from Candida-blood infections for diagnostic purposes

Dissection of RNAseq samples from Candida-blood infections for diagnostic purposes

In vitro, ex vivo and in vivo transcriptional profiles of all Candida species

In vitro ex vivo and in vivo transcriptional profiles of all Candida species

Analysis of the role of identified factors
In vitro, ex vivo Infection models established and characterized for all Candida species

In vitro ex vivo Infection models established and characterized for all Candida species

Improved proteomics-based diagnostics of pathogenic yeast species complexes

Improved proteomics-based diagnostics of pathogenic yeast species complexes

Validated biomarkers for resistant Candida isolates
Proteomics-based diagnostics of infected clinical materials
Proteomics-based diagnostics of resistant isolates

Publicaciones

Peer reviewed articles (25)

Proof of Concept for MBT ASTRA, a Rapid Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)-Based Method To Detect Caspofungin Resistance in Candida albicans and Candida glabrata

Autores: Mansoureh Vatanshenassan, Teun Boekhout, Cornelia Lass-Flörl, Michaela Lackner, Sören Schubert, Markus Kostrzewa, Katrin Sparbier
Publicado en: Journal of Clinical Microbiology, Edición 56/9, 2018, ISSN 0095-1137
Editor: American Society for Microbiology
DOI: 10.1128/JCM.00420-18

Low-Cost Tetraplex PCR for the Global Spreading Multi-Drug Resistant Fungus, Candida auris and Its Phylogenetic Relatives

Autores: Amir Arastehfar, Wenjie Fang, Hamid Badali, Afsane Vaezi, Weiwei Jiang, Wanqing Liao, Weihua Pan, Ferry Hagen, Teun Boekhout
Publicado en: Frontiers in Microbiology, Edición 9, 2018, ISSN 1664-302X
Editor: Frontiers Media
DOI: 10.3389/fmicb.2018.01119

nextPARS: parallel probing of RNA structures in Illumina

Autores: Ester Saus, Jesse R. Willis, Leszek P. Pryszcz, Ahmed Hafez, Carlos Llorens, Heinz Himmelbauer, Toni Gabaldón
Publicado en: RNA, Edición 24/4, 2018, Página(s) 609-619, ISSN 1355-8382
Editor: Cold Spring Harbor Laboratory Press
DOI: 10.1261/rna.063073.117

Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater

Autores: Antonio Rodríguez, Mario Vaneechoutte
Publicado en: BMC Microbiology, Edición 19/1, 2019, ISSN 1471-2180
Editor: BioMed Central
DOI: 10.1186/s12866-019-1473-z

Whole-Genome Sequencing of the Opportunistic Yeast Pathogen Candida inconspicua Uncovers Its Hybrid Origin

Autores: Verónica Mixão, Antonio Perez Hansen, Ester Saus, Teun Boekhout, Cornelia Lass-Florl, Toni Gabaldón
Publicado en: Frontiers in Genetics, Edición 10, 2019, ISSN 1664-8021
Editor: Frontiers Media
DOI: 10.3389/fgene.2019.00383

Misidentification of genome assemblies in public databases: The case of Naumovozyma dairenensis and proposal of a protocol to correct misidentifications

Autores: Aimilia A. Stavrou, Verónica Mixão, Teun Boekhout, Toni Gabaldón
Publicado en: Yeast, Edición 35/6, 2018, Página(s) 425-429, ISSN 0749-503X
Editor: John Wiley & Sons Inc.
DOI: 10.1002/yea.3303

Identification of an Exceptionally Long Intron in the HAC1 Gene of Candida parapsilosis

Autores: Elise Iracane, Paul D. Donovan, Mihaela Ola, Geraldine Butler, Linda M. Holland
Publicado en: mSphere, Edición 3/6, 2018, ISSN 2379-5042
Editor: American Society for Microbiology
DOI: 10.1128/msphere.00532-18

Candida auris Identification and Rapid Antifungal Susceptibility Testing Against Echinocandins by MALDI-TOF MS

Autores: Mansoureh Vatanshenassan, Teun Boekhout, Jacques F. Meis, Judith Berman, Anuradha Chowdhary, Ronen Ben-Ami, Katrin Sparbier, Markus Kostrzewa
Publicado en: Frontiers in Cellular and Infection Microbiology, Edición 9, 2019, ISSN 2235-2988
Editor: Frontiers Media S. A.
DOI: 10.3389/fcimb.2019.00020

Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species

Autores: Lisa Lombardi, João Oliveira-Pacheco, Geraldine Butler
Publicado en: mSphere, Edición 4/2, 2019, ISSN 2379-5042
Editor: American Society for Microbiology
DOI: 10.1128/msphere.00125-19

In vitro antifungal activity of amphotericin B and 11 comparators against Aspergillus terreus species complex

Autores: Afsane Vaezi, Hamed Fakhim, Amir Arastehfar, Tahereh Shokohi, Mohammad T. Hedayati, Sadegh Khodavaisy, Ali Rezaei-Matehkolaei, Parisa Badiee, Ferry Hagen, Cornelia Lass-Flörl, Eric Dannaoui, Jacques F. Meis, Hamid Badali
Publicado en: Mycoses, Edición 61/2, 2018, Página(s) 134-142, ISSN 0933-7407
Editor: Blackwell Publishing Inc.
DOI: 10.1111/myc.12716

Comparison of 21-Plex PCR and API 20C AUX, MALDI-TOF MS, and rDNA Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex PCR and API 20C AUX as an Alternative Strategy for Developing Countries

Autores: Amir Arastehfar, Farnaz Daneshnia, Mohammad Kord, Maryam Roudbary, Hossein Zarrinfar, Wenjie Fang, Sayed Jamal Hashemi, Mohammad Javad Najafzadeh, Sadegh Khodavaisy, Weihua Pan, Wanqing Liao, Hamid Badali, Sassan Rezaie, Kamiar Zomorodian, Ferry Hagen, Teun Boekhout
Publicado en: Frontiers in Cellular and Infection Microbiology, Edición 9, 2019, ISSN 2235-2988
Editor: Frontiers Media S. A.
DOI: 10.3389/fcimb.2019.00021

YEAST PANEL multiplex PCR for identification of clinically important yeast species: stepwise diagnostic strategy, useful for developing countries

Autores: Amir Arastehfar, Wenjie Fang, Weihua Pan, Michaela Lackner, Wanqing Liao, Parisa Badiee, Kamiar Zomorodian, Hamid Badali, Ferry Hagen, Cornelia Lass-Flörl, Teun Boekhout
Publicado en: Diagnostic Microbiology and Infectious Disease, Edición 93/2, 2019, Página(s) 112-119, ISSN 0732-8893
Editor: Elsevier BV
DOI: 10.1016/j.diagmicrobio.2018.09.007

Identification of nine cryptic species of Candida albicans, C. glabrata, and C. parapsilosis complexes using one-step multiplex PCR

Autores: Amir Arastehfar, Wenjie Fang, Weihua Pan, Wanqing Liao, Liang Yan, Teun Boekhout
Publicado en: BMC Infectious Diseases, Edición 18/1, 2018, ISSN 1471-2334
Editor: BioMed Central
DOI: 10.1186/s12879-018-3381-5

Novel multiplex real-time quantitative PCR detecting system approach for direct detection of Candida auris and its relatives in spiked serum samples

Autores: Amir Arastehfar, Wenjie Fang, Farnaz Daneshnia, Abdullah MS Al-Hatmi, Wanqing Liao, Weihua Pan, Ziauddin Khan, Suhail Ahmad, Katharina Rosam, Michaela Lackner, Cornelia Lass-Flörl, Ferry Hagen, Teun Boekhout
Publicado en: Future Microbiology, Edición 14/1, 2019, Página(s) 33-45, ISSN 1746-0913
Editor: Future Medicine Ltd.
DOI: 10.2217/fmb-2018-0227

Low level of antifungal resistance in Iranian isolates of Candida glabrata recovered from blood samples from multicenter (2015-2018): Potential prognostic values of genotyping and sequencing of PDR1

Autores: Amir Arastehfar, Farnaz Daneshnia, Kamiar Zomorodian, Mohammad-Javad Najafzadeh, Sadegh Khodavaisy, Hossein Zarrinfar, Ferry Hagen, Zahra Zare Shahrabadi, Michaela Lackner, Hossein Mirhendi, Mohammadreza Salehi, Maryam Roudbary, Weihua Pan, Markus Kostrzewa, Teun Boekhout
Publicado en: Antimicrobial Agents and Chemotherapy, 2019, ISSN 0066-4804
Editor: American Society for Microbiology
DOI: 10.1128/aac.02503-18

Recent trends in molecular diagnostics of yeast infections: from PCR to NGS

Autores: A Arastehfar, T Boekhout, G Butler, G Buda De Cesare, E Dolk, T Gabaldóln, A Hafez, B Hube, F Hagen, H Hovhannisyan, E Iracane, M Kostrzewa, M Lackner, C Lass-Flörl, C Llorens, V Mixão, C Munro, J Oliveira-Pacheco, M Pekmezovic, A Pérez-Hansen, A Rodriguez Sanchez, F M Sauer, K Sparbier, A A Stavrou, M Vaneechoutte, M Vatanshenassan, Toni Gabaldón
Publicado en: FEMS Microbiology Reviews, 2019, ISSN 0168-6445
Editor: Blackwell
DOI: 10.1093/femsre/fuz015

CROSSMAPPER: estimating cross-mapping rates and optimizing experimental design in multi-species sequencing studies

Autores: H Hovhannisyan, A Hafez, C Llorens, T Gabaldón
Publicado en: Bioinformatics, 2019, ISSN 1367-4803
Editor: Oxford University Press
DOI: 10.1093/bioinformatics/btz626

Correlating Genotype and Phenotype in the Asexual Yeast Candida orthopsilosis Implicates ZCF29 in Sensitivity to Caffeine

Autores: Kontxi Martinez de San Vicente, Markus S. Schröder, Lisa Lombardi, Elise Iracane, Geraldine Butler
Publicado en: "G3: Genes|Genomes|Genetics", 2019, Página(s) g3.400348.2019, ISSN 2160-1836
Editor: Genetics Society of America
DOI: 10.1534/g3.119.400348

Molecular characterization and antifungal susceptibility testing of Candida nivariensis from blood samples – an Iranian multicentre study and a review of the literature

Autores: Amir Arastehfar, Farnaz Daneshnia, Mohammad-Reza Salehi, Hossein Zarrinfar, Sadegh Khodavaisy, Pieter-Jan Haas, Maryam Roudbary, Mohammad-Javad Najafzadeh, Kamiar Zomorodian, Arezoo Charsizadeh, Carlo Brouwer, Weihua Pan, Ferry Hagen, Teun Boekhout
Publicado en: Journal of Medical Microbiology, Edición 68/5, 2019, Página(s) 770-777, ISSN 0022-2615
Editor: Society for General Microbiology
DOI: 10.1099/jmm.0.000963

Incidence and spectrum of yeast species isolated from the oral cavity of Iranian patients suffering from hematological malignancies

Autores: Amir Arastehfar, Farnaz Daneshnia, Shirin Farahyar, Wenjie Fang, Maryam Salimi, Mohammadreza Salehi, Ferry Hagen, Pan Weihua, Maryam Roudbary, Teun Boekhout
Publicado en: Journal of Oral Microbiology, Edición 11/1, 2019, Página(s) 1601061, ISSN 2000-2297
Editor: Co-Action Publishing
DOI: 10.1080/20002297.2019.1601061

First fungemia case due to environmental yeast Wickerhamomyces myanmarensis : detection by multiplex qPCR and antifungal susceptibility

Autores: Amir Arastehfar, Mina Bakhtiari, Farnaz Daneshnia, Wenjie Fang, Sara Khanjari Sadati, Abdullah MS Al-Hatmi, Marizeth Groenewald, Hamid Sharifi-Mehr, Wanqing Liao, Weihua Pan, Kamiar Zomorodian, Ferry Hagen, Teun Boekhout
Publicado en: Future Microbiology, Edición 14/4, 2019, Página(s) 267-274, ISSN 1746-0913
Editor: Future Medicine Ltd.
DOI: 10.2217/fmb-2018-0253

Molecular Identification, Genotypic Diversity, Antifungal Susceptibility, and Clinical Outcomes of Infections Caused by Clinically Underrated Yeasts, Candida orthopsilosis, and Candida metapsilosis: An Iranian Multicenter Study (2014–2019)

Autores: Amir Arastehfar, Sadegh Khodavaisy, Farnaz Daneshnia, Mohammad-Javad Najafzadeh, Shahram Mahmoudi, Arezoo Charsizadeh, Mohammad-Reza Salehi, Hossein Zarrinfar, Abbas Raeisabadi, Somayeh Dolatabadi, Zahra Zare Shahrabadi, Kamiar Zomorodian, Weihua Pan, Ferry Hagen, Teun Boekhout
Publicado en: Frontiers in Cellular and Infection Microbiology, Edición 9, 2019, ISSN 2235-2988
Editor: Frontiers Media S. A.
DOI: 10.3389/fcimb.2019.00264

Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions

Autores: Antonio Rodríguez, Brecht Guillemyn, Paul Coucke, Mario Vaneechoutte
Publicado en: Scientific Reports, Edición 9/1, 2019, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-019-54608-x

Effects of the glucocorticoid betamethasone on the interaction of Candida albicans with human epithelial cells

Autores: Ágnes Jakab, Selene Mogavero, Toni M. Förster​, Marina Pekmezovic​, Nadja Jablonowski​, Viktor Dombrádi​, István Pócsi​, Bernhard Hube
Publicado en: Microbiology, Edición 162/12, 2016, Página(s) 2116-2125, ISSN 1350-0872
Editor: Society for General Microbiology
DOI: 10.1099/mic.0.000383

Hybridization and emergence of virulence in opportunistic human yeast pathogens

Autores: Verónica Mixão, Toni Gabaldón
Publicado en: Yeast, 2017, ISSN 0749-503X
Editor: John Wiley & Sons Inc.
DOI: 10.1002/yea.3242

Book chapters (1)

Transcriptome Sequencing Approaches to Elucidate Host–Microbe Interactions in Opportunistic Human Fungal Pathogens

Autores: Hrant Hovhannisyan, Toni Gabaldón
Publicado en: Current Topics in Microbiology and Immunology, 2018
Editor: Springer Berlin Heidelberg
DOI: 10.1007/82_2018_122