Deliverables Documents, reports (15) Cellular and in vivo delivery of RNA-based sensor Protocol for efficient in vitro cellular and in vivo delivery of RNA-based sensors First flux-sensor validated A first flux-sensor validated Elucidation of population wide production heterogeneity on the single cell level Elucidation of population wide production heterogeneity on the single cell level for thiamine or coumarate cell factory on different fermentation scales Quantitative MS and IMS Combined MS and IMS methods to screen RNA libraries and characterize the ligand binding affinities and the induced conformational changes Fluxes through the yeast cell cycle quantified Fluxes through the yeast cell cycle quantified RNA-based metabolite sensor RNA-based sensor for fructose-1,6-bisphosphate Optimized RNA-based sensor Optimized RNA-based sensors Novel SELEX method Novel SELEX method for identification of RNA molecules that bind to riboswitches First flux-signaling metabolites identified List with potential flux-signaling metabolites in yeast central metabolites RNA-based sensor for thiamine or coumarate RNA-based sensor for thiamine or coumarate sensing intracellular concentrations in the range 50-5000 ug/L First aptamers identified Aptamers identified for required metabolites Adenoviral vectors Adenoviral vectors that allow delivery of aptamers to mouse liver Methodology to optimize RNA-based sensors Description of methodologies for the computational design and directed evolution of RNA-based sensors Characterisation of normal liver & liver tumour metabolic heterogeneity Delivery of aptamers to mouse cancer models of liver cancer and characterization of organ and tumour metabolic heterogeneity Secondary structure of aptamers in vivo Secondary structure of a selected set of metabolite-sensing aptamers both in vitro and in vivo Other (5) Highest producing yeast strains Selection (using RNA-based sensor) and biochemical characterization of highest producing yeast strain Test of application of titratable RNA-based sensor Test of application of titratable RNA-based sensor for sequential optimization of thiamine cell factory Highest producing E.coli strains Selection (using RNA-based sensor) and biochemical characterization of highest producing E.coli strains following combinatorial metabolic engineering Scientific courses Organization and attendance of scientific courses SC1-SC4 (M8,12,18,24) Lead participant:All Transferable skills courses Organization and attendance of courses on transferable skills TS1-TS3 (M18,24 and 30) Lead participant: All Demonstrators, pilots, prototypes (1) In vivo screening system Development of screening system for the optimization of metabolite-sensing aptamers Websites, patent fillings, videos etc. (1) Project website Project website online (M3) Publications Peer reviewed articles (16) Mechanistic insights into type I toxin antitoxin systems in Helicobacter pylori: the importance of mRNA folding in controlling toxin expression Author(s): Hélène Arnion, Dursun Nizam Korkut, Sara Masachis Gelo, Sandrine Chabas, Jérémy Reignier, Isabelle Iost, Fabien Darfeuille Published in: Nucleic Acids Research, 2017, Page(s) gkw1343, ISSN 0305-1048 Publisher: Oxford University Press DOI: 10.1093/nar/gkw1343 Tumour microenvironment factors shaping the cancer metabolism landscape Author(s): Dimitrios Anastasiou Published in: British Journal of Cancer, Issue 116/3, 2016, Page(s) 277-286, ISSN 0007-0920 Publisher: Nature Publishing Group DOI: 10.1038/bjc.2016.412 Using RNA as Molecular Code for Programming Cellular Function Author(s): Manish Kushwaha, William Rostain, Satya Prakash, John N. Duncan, Alfonso Jaramillo Published in: ACS Synthetic Biology, Issue 5/8, 2016, Page(s) 795-809, ISSN 2161-5063 Publisher: American Chemical Society DOI: 10.1021/acssynbio.5b00297 Next-level riboswitch development—implementation of Capture-SELEX facilitates identification of a new synthetic riboswitch Author(s): Adrien Boussebayle, Daniel Torka, Sandra Ollivaud, Johannes Braun, Cristina Bofill-Bosch, Max Dombrowski, Florian Groher, Kay Hamacher, Beatrix Suess Published in: Nucleic Acids Research, Issue 47/9, 2019, Page(s) 4883-4895, ISSN 0305-1048 Publisher: Oxford University Press DOI: 10.1093/nar/gkz216 Adaptive Laboratory Evolution of Antibiotic Resistance Using Different Selection Regimes Lead to Similar Phenotypes and Genotypes Author(s): Leonie J. Jahn, Christian Munck, Mostafa M. H. Ellabaan, Morten O. A. Sommer Published in: Frontiers in Microbiology, Issue 8, 2017, ISSN 1664-302X Publisher: Frontiers Media DOI: 10.3389/fmicb.2017.00816 Wiring cell growth to product formation Author(s): Josi Buerger, Luisa S. Gronenberg, Hans Jasper Genee, Morten O.A. Sommer Published in: Current Opinion in Biotechnology, Issue 59, 2019, Page(s) 85-92, ISSN 0958-1669 Publisher: Elsevier BV DOI: 10.1016/j.copbio.2019.02.014 Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan Author(s): Simeon Leupold, Georg Hubmann, Athanasios Litsios, Anne C Meinema, Vakil Takhaveev, Alexandros Papagiannakis, Bastian Niebel, Georges Janssens, David Siegel, Matthias Heinemann Published in: eLife, Issue 8, 2019, ISSN 2050-084X Publisher: eLife Sciences Publications DOI: 10.7554/elife.41046 RNA polymerase III limits longevity downstream of TORC1 Author(s): Danny Filer, Maximillian A. Thompson, Vakil Takhaveev, Adam J. Dobson, Ilektra Kotronaki, James W. M. Green, Matthias Heinemann, Jennifer M. A. Tullet, Nazif Alic Published in: Nature, Issue 552/7684, 2017, Page(s) 263-267, ISSN 0028-0836 Publisher: Nature Publishing Group DOI: 10.1038/nature25007 MCT2 mediates concentration-dependent inhibition of glutamine metabolism by MOG Author(s): Louise Fets, Paul C. Driscoll, Fiona Grimm, Aakriti Jain, Patrícia M. Nunes, Michalis Gounis, Ginevra Doglioni, George Papageorgiou, Timothy J. Ragan, Sebastien Campos, Mariana Silva dos Santos, James I. MacRae, Nicola O’Reilly, Alan J. Wright, Cyril H. Benes, Kevin D. Courtney, David House, Dimitrios Anastasiou Published in: Nature Chemical Biology, Issue 14/11, 2018, Page(s) 1032-1042, ISSN 1552-4450 Publisher: Nature Publishing Group DOI: 10.1038/s41589-018-0136-y Chromosomal barcoding as a tool for multiplexed phenotypic characterization of laboratory evolved lineages Author(s): Leonie Johanna Jahn, Andreas Porse, Christian Munck, Daniel Simon, Svetlana Volkova, Morten Otto Alexander Sommer Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322 Publisher: Nature Publishing Group DOI: 10.1038/s41598-018-25201-5 Metabolic heterogeneity in clonal microbial populations Author(s): Vakil Takhaveev, Matthias Heinemann Published in: Current Opinion in Microbiology, Issue 45, 2018, Page(s) 30-38, ISSN 1369-5274 Publisher: Elsevier BV DOI: 10.1016/j.mib.2018.02.004 Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection Author(s): Stefan Breuers, Laura Lledo Bryant, Tjasa Legen, Günter Mayer Published in: Methods, Issue 161, 2019, Page(s) 3-9, ISSN 1046-2023 Publisher: Academic Press DOI: 10.1016/j.ymeth.2019.05.022 Pharmacogenomics of GPCR Drug Targets Author(s): Alexander S. Hauser, Sreenivas Chavali, Ikuo Masuho, Leonie J. Jahn, Kirill A. Martemyanov, David E. Gloriam, M. Madan Babu Published in: Cell, Issue 172/1-2, 2018, Page(s) 41-54.e19, ISSN 0092-8674 Publisher: Cell Press DOI: 10.1016/j.cell.2017.11.033 A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA Author(s): Sara Masachis, Nicolas J Tourasse, Claire Lays, Marion Faucher, Sandrine Chabas, Isabelle Iost, Fabien Darfeuille Published in: eLife, Issue 8, 2019, ISSN 2050-084X Publisher: eLife Sciences Publications DOI: 10.7554/elife.47549 Discovery and Characterization of Cas9 Inhibitors Disseminated across Seven Bacterial Phyla Author(s): Ruben V. Uribe, Eric van der Helm, Maria-Anna Misiakou, Sang-Woo Lee, Stefan Kol, Morten O.A. Sommer Published in: Cell Host & Microbe, Issue 25/2, 2019, Page(s) 233-241.e5, ISSN 1931-3128 Publisher: Cell Press DOI: 10.1016/j.chom.2019.01.003 Robust gene expression control in human cells with a novel universal TetR aptamer splicing module Author(s): Adam A Mol, Florian Groher, Britta Schreiber, Ciaran Rühmkorff, Beatrix Suess Published in: Nucleic acid research, Issue Sep 2, 2019, Page(s) gkz753, ISSN 0305-1048 Publisher: Oxford University Press DOI: 10.1093/nar/gkz753 Searching for OpenAIRE data... There was an error trying to search data from OpenAIRE No results available
