Livrables
Protocol for efficient in vitro cellular and in vivo delivery of RNA-based sensors
First flux-sensor validatedA first flux-sensor validated
Elucidation of population wide production heterogeneity on the single cell levelElucidation of population wide production heterogeneity on the single cell level for thiamine or coumarate cell factory on different fermentation scales
Quantitative MS and IMSCombined MS and IMS methods to screen RNA libraries and characterize the ligand binding affinities and the induced conformational changes
Fluxes through the yeast cell cycle quantifiedRNA-based metabolite sensor
RNA-based sensor for fructose-1,6-bisphosphate
Optimized RNA-based sensorOptimized RNA-based sensors
Novel SELEX methodNovel SELEX method for identification of RNA molecules that bind to riboswitches
First flux-signaling metabolites identifiedList with potential flux-signaling metabolites in yeast central metabolites
RNA-based sensor for thiamine or coumarateRNA-based sensor for thiamine or coumarate sensing intracellular concentrations in the range 50-5000 ug/L
First aptamers identifiedAptamers identified for required metabolites
Adenoviral vectorsAdenoviral vectors that allow delivery of aptamers to mouse liver
Methodology to optimize RNA-based sensorsDescription of methodologies for the computational design and directed evolution of RNA-based sensors
Characterisation of normal liver & liver tumour metabolic heterogeneityDelivery of aptamers to mouse cancer models of liver cancer and characterization of organ and tumour metabolic heterogeneity
Secondary structure of aptamers in vivoSecondary structure of a selected set of metabolite-sensing aptamers both in vitro and in vivo
Selection (using RNA-based sensor) and biochemical characterization of highest producing yeast strain
Test of application of titratable RNA-based sensorTest of application of titratable RNA-based sensor for sequential optimization of thiamine cell factory
Highest producing E.coli strainsSelection (using RNA-based sensor) and biochemical characterization of highest producing E.coli strains following combinatorial metabolic engineering
Scientific coursesOrganization and attendance of scientific courses SC1-SC4 (M8,12,18,24) Lead participant:All
Transferable skills coursesOrganization and attendance of courses on transferable skills TS1-TS3 (M18,24 and 30) Lead participant: All
Development of screening system for the optimization of metabolite-sensing aptamers
Project website online (M3)
Publications
Auteurs:
Hélène Arnion, Dursun Nizam Korkut, Sara Masachis Gelo, Sandrine Chabas, Jérémy Reignier, Isabelle Iost, Fabien Darfeuille
Publié dans:
Nucleic Acids Research, 2017, Page(s) gkw1343, ISSN 0305-1048
Éditeur:
Oxford University Press
DOI:
10.1093/nar/gkw1343
Auteurs:
Dimitrios Anastasiou
Publié dans:
British Journal of Cancer, Numéro 116/3, 2016, Page(s) 277-286, ISSN 0007-0920
Éditeur:
Nature Publishing Group
DOI:
10.1038/bjc.2016.412
Auteurs:
Manish Kushwaha, William Rostain, Satya Prakash, John N. Duncan, Alfonso Jaramillo
Publié dans:
ACS Synthetic Biology, Numéro 5/8, 2016, Page(s) 795-809, ISSN 2161-5063
Éditeur:
American Chemical Society
DOI:
10.1021/acssynbio.5b00297
Auteurs:
Adrien Boussebayle, Daniel Torka, Sandra Ollivaud, Johannes Braun, Cristina Bofill-Bosch, Max Dombrowski, Florian Groher, Kay Hamacher, Beatrix Suess
Publié dans:
Nucleic Acids Research, Numéro 47/9, 2019, Page(s) 4883-4895, ISSN 0305-1048
Éditeur:
Oxford University Press
DOI:
10.1093/nar/gkz216
Auteurs:
Leonie J. Jahn, Christian Munck, Mostafa M. H. Ellabaan, Morten O. A. Sommer
Publié dans:
Frontiers in Microbiology, Numéro 8, 2017, ISSN 1664-302X
Éditeur:
Frontiers Media
DOI:
10.3389/fmicb.2017.00816
Auteurs:
Josi Buerger, Luisa S. Gronenberg, Hans Jasper Genee, Morten O.A. Sommer
Publié dans:
Current Opinion in Biotechnology, Numéro 59, 2019, Page(s) 85-92, ISSN 0958-1669
Éditeur:
Elsevier BV
DOI:
10.1016/j.copbio.2019.02.014
Auteurs:
Simeon Leupold, Georg Hubmann, Athanasios Litsios, Anne C Meinema, Vakil Takhaveev, Alexandros Papagiannakis, Bastian Niebel, Georges Janssens, David Siegel, Matthias Heinemann
Publié dans:
eLife, Numéro 8, 2019, ISSN 2050-084X
Éditeur:
eLife Sciences Publications
DOI:
10.7554/elife.41046
Auteurs:
Danny Filer, Maximillian A. Thompson, Vakil Takhaveev, Adam J. Dobson, Ilektra Kotronaki, James W. M. Green, Matthias Heinemann, Jennifer M. A. Tullet, Nazif Alic
Publié dans:
Nature, Numéro 552/7684, 2017, Page(s) 263-267, ISSN 0028-0836
Éditeur:
Nature Publishing Group
DOI:
10.1038/nature25007
Auteurs:
Louise Fets, Paul C. Driscoll, Fiona Grimm, Aakriti Jain, Patrícia M. Nunes, Michalis Gounis, Ginevra Doglioni, George Papageorgiou, Timothy J. Ragan, Sebastien Campos, Mariana Silva dos Santos, James I. MacRae, Nicola O’Reilly, Alan J. Wright, Cyril H. Benes, Kevin D. Courtney, David House, Dimitrios Anastasiou
Publié dans:
Nature Chemical Biology, Numéro 14/11, 2018, Page(s) 1032-1042, ISSN 1552-4450
Éditeur:
Nature Publishing Group
DOI:
10.1038/s41589-018-0136-y
Auteurs:
Leonie Johanna Jahn, Andreas Porse, Christian Munck, Daniel Simon, Svetlana Volkova, Morten Otto Alexander Sommer
Publié dans:
Scientific Reports, Numéro 8/1, 2018, ISSN 2045-2322
Éditeur:
Nature Publishing Group
DOI:
10.1038/s41598-018-25201-5
Auteurs:
Vakil Takhaveev, Matthias Heinemann
Publié dans:
Current Opinion in Microbiology, Numéro 45, 2018, Page(s) 30-38, ISSN 1369-5274
Éditeur:
Elsevier BV
DOI:
10.1016/j.mib.2018.02.004
Auteurs:
Stefan Breuers, Laura Lledo Bryant, Tjasa Legen, Günter Mayer
Publié dans:
Methods, Numéro 161, 2019, Page(s) 3-9, ISSN 1046-2023
Éditeur:
Academic Press
DOI:
10.1016/j.ymeth.2019.05.022
Auteurs:
Alexander S. Hauser, Sreenivas Chavali, Ikuo Masuho, Leonie J. Jahn, Kirill A. Martemyanov, David E. Gloriam, M. Madan Babu
Publié dans:
Cell, Numéro 172/1-2, 2018, Page(s) 41-54.e19, ISSN 0092-8674
Éditeur:
Cell Press
DOI:
10.1016/j.cell.2017.11.033
Auteurs:
Sara Masachis, Nicolas J Tourasse, Claire Lays, Marion Faucher, Sandrine Chabas, Isabelle Iost, Fabien Darfeuille
Publié dans:
eLife, Numéro 8, 2019, ISSN 2050-084X
Éditeur:
eLife Sciences Publications
DOI:
10.7554/elife.47549
Auteurs:
Ruben V. Uribe, Eric van der Helm, Maria-Anna Misiakou, Sang-Woo Lee, Stefan Kol, Morten O.A. Sommer
Publié dans:
Cell Host & Microbe, Numéro 25/2, 2019, Page(s) 233-241.e5, ISSN 1931-3128
Éditeur:
Cell Press
DOI:
10.1016/j.chom.2019.01.003
Auteurs:
Adam A Mol, Florian Groher, Britta Schreiber, Ciaran Rühmkorff, Beatrix Suess
Publié dans:
Nucleic acid research, Numéro Sep 2, 2019, Page(s) gkz753, ISSN 0305-1048
Éditeur:
Oxford University Press
DOI:
10.1093/nar/gkz753
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