Resultado final
Protocol for efficient in vitro cellular and in vivo delivery of RNA-based sensors
First flux-sensor validatedA first flux-sensor validated
Elucidation of population wide production heterogeneity on the single cell levelElucidation of population wide production heterogeneity on the single cell level for thiamine or coumarate cell factory on different fermentation scales
Quantitative MS and IMSCombined MS and IMS methods to screen RNA libraries and characterize the ligand binding affinities and the induced conformational changes
Fluxes through the yeast cell cycle quantifiedRNA-based metabolite sensor
RNA-based sensor for fructose-1,6-bisphosphate
Optimized RNA-based sensorOptimized RNA-based sensors
Novel SELEX methodNovel SELEX method for identification of RNA molecules that bind to riboswitches
First flux-signaling metabolites identifiedList with potential flux-signaling metabolites in yeast central metabolites
RNA-based sensor for thiamine or coumarateRNA-based sensor for thiamine or coumarate sensing intracellular concentrations in the range 50-5000 ug/L
First aptamers identifiedAptamers identified for required metabolites
Adenoviral vectorsAdenoviral vectors that allow delivery of aptamers to mouse liver
Methodology to optimize RNA-based sensorsDescription of methodologies for the computational design and directed evolution of RNA-based sensors
Characterisation of normal liver & liver tumour metabolic heterogeneityDelivery of aptamers to mouse cancer models of liver cancer and characterization of organ and tumour metabolic heterogeneity
Secondary structure of aptamers in vivoSecondary structure of a selected set of metabolite-sensing aptamers both in vitro and in vivo
Selection (using RNA-based sensor) and biochemical characterization of highest producing yeast strain
Test of application of titratable RNA-based sensorTest of application of titratable RNA-based sensor for sequential optimization of thiamine cell factory
Highest producing E.coli strainsSelection (using RNA-based sensor) and biochemical characterization of highest producing E.coli strains following combinatorial metabolic engineering
Scientific coursesOrganization and attendance of scientific courses SC1-SC4 (M8,12,18,24) Lead participant:All
Transferable skills coursesOrganization and attendance of courses on transferable skills TS1-TS3 (M18,24 and 30) Lead participant: All
Development of screening system for the optimization of metabolite-sensing aptamers
Project website online (M3)
Publicaciones
Autores:
Hélène Arnion, Dursun Nizam Korkut, Sara Masachis Gelo, Sandrine Chabas, Jérémy Reignier, Isabelle Iost, Fabien Darfeuille
Publicado en:
Nucleic Acids Research, 2017, Página(s) gkw1343, ISSN 0305-1048
Editor:
Oxford University Press
DOI:
10.1093/nar/gkw1343
Autores:
Dimitrios Anastasiou
Publicado en:
British Journal of Cancer, Edición 116/3, 2016, Página(s) 277-286, ISSN 0007-0920
Editor:
Nature Publishing Group
DOI:
10.1038/bjc.2016.412
Autores:
Manish Kushwaha, William Rostain, Satya Prakash, John N. Duncan, Alfonso Jaramillo
Publicado en:
ACS Synthetic Biology, Edición 5/8, 2016, Página(s) 795-809, ISSN 2161-5063
Editor:
American Chemical Society
DOI:
10.1021/acssynbio.5b00297
Autores:
Adrien Boussebayle, Daniel Torka, Sandra Ollivaud, Johannes Braun, Cristina Bofill-Bosch, Max Dombrowski, Florian Groher, Kay Hamacher, Beatrix Suess
Publicado en:
Nucleic Acids Research, Edición 47/9, 2019, Página(s) 4883-4895, ISSN 0305-1048
Editor:
Oxford University Press
DOI:
10.1093/nar/gkz216
Autores:
Leonie J. Jahn, Christian Munck, Mostafa M. H. Ellabaan, Morten O. A. Sommer
Publicado en:
Frontiers in Microbiology, Edición 8, 2017, ISSN 1664-302X
Editor:
Frontiers Media
DOI:
10.3389/fmicb.2017.00816
Autores:
Josi Buerger, Luisa S. Gronenberg, Hans Jasper Genee, Morten O.A. Sommer
Publicado en:
Current Opinion in Biotechnology, Edición 59, 2019, Página(s) 85-92, ISSN 0958-1669
Editor:
Elsevier BV
DOI:
10.1016/j.copbio.2019.02.014
Autores:
Simeon Leupold, Georg Hubmann, Athanasios Litsios, Anne C Meinema, Vakil Takhaveev, Alexandros Papagiannakis, Bastian Niebel, Georges Janssens, David Siegel, Matthias Heinemann
Publicado en:
eLife, Edición 8, 2019, ISSN 2050-084X
Editor:
eLife Sciences Publications
DOI:
10.7554/elife.41046
Autores:
Danny Filer, Maximillian A. Thompson, Vakil Takhaveev, Adam J. Dobson, Ilektra Kotronaki, James W. M. Green, Matthias Heinemann, Jennifer M. A. Tullet, Nazif Alic
Publicado en:
Nature, Edición 552/7684, 2017, Página(s) 263-267, ISSN 0028-0836
Editor:
Nature Publishing Group
DOI:
10.1038/nature25007
Autores:
Louise Fets, Paul C. Driscoll, Fiona Grimm, Aakriti Jain, Patrícia M. Nunes, Michalis Gounis, Ginevra Doglioni, George Papageorgiou, Timothy J. Ragan, Sebastien Campos, Mariana Silva dos Santos, James I. MacRae, Nicola O’Reilly, Alan J. Wright, Cyril H. Benes, Kevin D. Courtney, David House, Dimitrios Anastasiou
Publicado en:
Nature Chemical Biology, Edición 14/11, 2018, Página(s) 1032-1042, ISSN 1552-4450
Editor:
Nature Publishing Group
DOI:
10.1038/s41589-018-0136-y
Autores:
Leonie Johanna Jahn, Andreas Porse, Christian Munck, Daniel Simon, Svetlana Volkova, Morten Otto Alexander Sommer
Publicado en:
Scientific Reports, Edición 8/1, 2018, ISSN 2045-2322
Editor:
Nature Publishing Group
DOI:
10.1038/s41598-018-25201-5
Autores:
Vakil Takhaveev, Matthias Heinemann
Publicado en:
Current Opinion in Microbiology, Edición 45, 2018, Página(s) 30-38, ISSN 1369-5274
Editor:
Elsevier BV
DOI:
10.1016/j.mib.2018.02.004
Autores:
Stefan Breuers, Laura Lledo Bryant, Tjasa Legen, Günter Mayer
Publicado en:
Methods, Edición 161, 2019, Página(s) 3-9, ISSN 1046-2023
Editor:
Academic Press
DOI:
10.1016/j.ymeth.2019.05.022
Autores:
Alexander S. Hauser, Sreenivas Chavali, Ikuo Masuho, Leonie J. Jahn, Kirill A. Martemyanov, David E. Gloriam, M. Madan Babu
Publicado en:
Cell, Edición 172/1-2, 2018, Página(s) 41-54.e19, ISSN 0092-8674
Editor:
Cell Press
DOI:
10.1016/j.cell.2017.11.033
Autores:
Sara Masachis, Nicolas J Tourasse, Claire Lays, Marion Faucher, Sandrine Chabas, Isabelle Iost, Fabien Darfeuille
Publicado en:
eLife, Edición 8, 2019, ISSN 2050-084X
Editor:
eLife Sciences Publications
DOI:
10.7554/elife.47549
Autores:
Ruben V. Uribe, Eric van der Helm, Maria-Anna Misiakou, Sang-Woo Lee, Stefan Kol, Morten O.A. Sommer
Publicado en:
Cell Host & Microbe, Edición 25/2, 2019, Página(s) 233-241.e5, ISSN 1931-3128
Editor:
Cell Press
DOI:
10.1016/j.chom.2019.01.003
Autores:
Adam A Mol, Florian Groher, Britta Schreiber, Ciaran Rühmkorff, Beatrix Suess
Publicado en:
Nucleic acid research, Edición Sep 2, 2019, Página(s) gkz753, ISSN 0305-1048
Editor:
Oxford University Press
DOI:
10.1093/nar/gkz753
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