CORDIS - Risultati della ricerca dell’UE
CORDIS

Improved production strategies for endangered freshwater species.

Risultati finali

Socio-economic dimensions

A key requisite to implement important project findings into real life fishery management and conservation is to gain knowledge and understanding on how key stakeholders assess the overall findings and recommendations from the project, especially the recommended “paradigm shift” in fish cultivation practices compared to current practices and established historic traditions. Further, it is necessary to evaluate the potential economic benefits of successful restoration and conservation of these iconic species to design economically efficient and socially acceptable programs. We will study how key stakeholders in fishery management, conservation, stocking and angling activities assess and make trade-offs between pros and cons of existing and new production techniques. Further, we will map and quantify the potential economic and social benefits (individual, social/cultural, and economic) of successful restoration and conservation of iconic species Atlantic salmon, European eel, and sturgeon at the level of local communities in Germany, France and Norway.

Innovative production strategies for fish conservation and management

In response to the dramatic decline in wild stocks of many of the world´s fish species, many attempts have been made to bolster declining stocks by releasing hatchery-reared juveniles back into the wild. However, current intensive hatchery procedures result in behaviourally naive juveniles which display physiological adaptations towards hatchery rearing conditions. These production deficiencies results in reduced survival and reproduction in the wild, and adapted rearing practices are required to increase the overall fitness under natural conditions instead of a focus on performance in a near constant commercial rearing environment. New production protocols will be designed that will increase the cognitive ability and physiological capabilities of fish as compared to those raised under traditional intensive hatchery conditions. This calls for a paradigm shift in hatchery management, moving away from traditional husbandry practices to new hatchery procedures (optimal stocking density, adapted fertilization strategies, environmental enrichment, variable feeding, variable physical parameters such as flow rate, direction of flow, temperature) aimed at increasing the physiological fitness and cognitive ability of raised fish, which in turn leads to increased post-release survival (stock enhancement), as well as improved release strategies.

Improved Broodstock Management

Broodstock management involves all the appropriate measures taken by the farmer to enable a captive group of fish to undergo reproductive maturation and spawning. Currently, hormonal manipulation is commonly used as a management tool in the cultivation of the targeted species (eel, salmon and sturgeons), either to enhance the efficiency of egg production or to increase spermiation, both facilitating hatchery operations. We will set up new protocols for both gamete development and spawning induction of broodstock, through development of alternative treatments (including environmental changes like photoperiod or temperature, or gonadotropin usage (homologous pituitary hormones, recombinant hormones, neuropeptides) for inducing gamete production.

Improved Gamete Quality and Management

European eel and sturgeons are included in the IUCN as Critically Endangered species. To reduce fishing pressure, and the dependency of this industry on wild populations, and also for stocking purposes, it is important to successfully close the life-cycle of the eel in captivity. Improved gamete quality and management will also benefit production of wildtype conditioned sturgeon and salmon. At present, it is possible to obtain eel eggs or sperm for in vitro fertilization, but only after and very expensive hormonal treatments. Using environmental treatments or gonadotropin implants we plan to reduce or partially replace the standard hormonal treatments currently used to achieve sexual maturation, as well as obtaining a better gamete quality. Furthermore, there is a need to keep good quality sperm reserves in order to fertilize all the eggs when a good batch is obtained. Thus, short-term preservation and cryopreservation methods are required. Utilization of innovative transplantation techniques can help the development of gametes extracted from endangered species using more common species (shorter age of maturation) as the surrogate host. Furthermore, the use of germ cells for the creation of germline chimera is an expansion of this technique for application in cryopreservation platforms aimed at conservation of threatened genetic resources.

New in vitro and –omics technologies

The complete genome sequence of Acipenser sturio will be mapped using an established de novo assembly pipeline and multiple tissue samples will be used to generate reference transcriptome profiles. Subsequently, these genomic resources will be used to develop new applications: (1) blood and fin clip samples obtained during artificial maturation trials will be used to generate minimally invasive maturation markers, the predictive value of which will be determined in a dedicated validation trial; (2) modified gonadotropins and neuronal signaling molecules will be designed for optimized artificial maturation of A. sturio; (3) molecular information about hormones and their receptors will allow the development of in vitro model systems for regulatory mechanism investigations, for alternatives to in vivo experiments and for reduction of number of animals used.

Website development

An Impress website will be set up for efficient communication of results and activities to the public to the scientific community and between the consortium members

Pubblicazioni

Gonadotropins in the Russian Sturgeon: Their Role in Steroid Secretion and the Effect of Hormonal Treatment on Their Secretion

Autori: Svetlana Yom-Din, Lian Hollander-Cohen, Joseph Aizen, Benjamin Boehm, Michal Shpilman, Matan Golan, Avshalom Hurvitz, Gad Degani, Berta Levavi-Sivan
Pubblicato in: PLOS ONE, Numero 11/9, 2016, Pagina/e e0162344, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0162344

Nuclear and membrane progestin receptors in the European eel: Characterization and expression in vivo through spermatogenesis

Autori: Marina Morini, David S. Peñaranda, María C. Vílchez, Rasoul Nourizadeh-Lillabadi, Anne-Gaëlle Lafont, Sylvie Dufour, Juan F. Asturiano, Finn-Arne Weltzien, Luz Pérez
Pubblicato in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Numero 207, 2017, Pagina/e 79-92, ISSN 1095-6433
Editore: Elsevier BV
DOI: 10.1016/j.cbpa.2017.02.009

Rapid de novo assembly of the European eel genome from nanopore sequencing reads

Autori: Hans J. Jansen, Michael Liem, Susanne A. Jong-Raadsen, Sylvie Dufour, Finn-Arne Weltzien, William Swinkels, Alex Koelewijn, Arjan P. Palstra, Bernd Pelster, Herman P. Spaink, Guido E. van den Thillart, Ron P. Dirks, Christiaan V. Henkel
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-017-07650-6

Handling and Treatment of Male European Eels (<em>Anguilla anguilla</em>)for Hormonal Maturation and Sperm Cryopreservation

Autori: Juan G. Herranz-Jusdado, Eszter Kása, Tímea Kollár, Víctor Gallego, David S. Peñaranda, Christoffer Rozenfeld, Luz Pérez, Ákos Horváth, Juan F. Asturiano
Pubblicato in: Journal of Visualized Experiments, Numero 131, 2018, ISSN 1940-087X
Editore: MYJoVE Corporation
DOI: 10.3791/56835

The expression of nuclear and membrane estrogen receptors in the European eel throughout spermatogenesis

Autori: Marina Morini, David S. Peñaranda, M. Carmen Vílchez, Helge Tveiten, Anne-Gaëlle Lafont, Sylvie Dufour, Luz Pérez, Juan F. Asturiano
Pubblicato in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Numero 203, 2017, Pagina/e 91-99, ISSN 1095-6433
Editore: Elsevier BV
DOI: 10.1016/j.cbpa.2016.08.020

Using specific recombinant gonadotropins to induce spermatogenesis and spermiation in the European eel ( Anguilla anguilla )

Autori: D.S. Peñaranda, V. Gallego, C. Rozenfeld, J.G. Herranz-Jusdado, L. Pérez, A. Gómez, I. Giménez, J.F. Asturiano
Pubblicato in: Theriogenology, Numero 107, 2018, Pagina/e 6-20, ISSN 0093-691X
Editore: Elsevier BV
DOI: 10.1016/j.theriogenology.2017.11.002

Hatching Knowledge: A Case Study on the Hybridization of Local Ecological Knowledge and Scientific Knowledge in Small-Scale Atlantic Salmon (Salmo salar) Cultivation in Norway

Autori: Hannah L. Harrison, Stine Rybråten, Øystein Aas
Pubblicato in: Human Ecology, Numero 46/4, 2018, Pagina/e 449-459, ISSN 0300-7839
Editore: Springer
DOI: 10.1007/s10745-018-0001-3

“Nature’s Little Helpers”: A benefits approach to voluntary cultivation of hatchery fish to support wild Atlantic salmon ( Salmo salar ) populations in Norway, Wales, and Germany

Autori: Hannah L. Harrison, Sophia Kochalski, Robert Arlinghaus, Øystein Aas
Pubblicato in: Fisheries Research, Numero 204, 2018, Pagina/e 348-360, ISSN 0165-7836
Editore: Elsevier BV
DOI: 10.1016/j.fishres.2018.02.022

Comparison of sperm motility subpopulation structure among wild anadromous and farmed male Atlantic salmon (Salmo salar) parr using a CASA system

Autori: Carina Caldeira, Almudena García-Molina, Anthony Valverde, Daznia Bompart, Megan Hassane, Patrick Martin, Carles Soler
Pubblicato in: Reproduction, Fertility and Development, Numero 30/6, 2018, Pagina/e 897, ISSN 1031-3613
Editore: Commonwealth Scientific and Industrial Research Organization Publishing
DOI: 10.1071/RD17466

CASA-Mot technology: how results are affected by the frame rate and counting chamber

Autori: Daznia Bompart, Almudena García-Molina, Anthony Valverde, Carina Caldeira, Jesús Yániz, Manuel Núñez de Murga, Carles Soler
Pubblicato in: Reproduction, Fertility and Development, Numero 30/6, 2018, Pagina/e 810, ISSN 1031-3613
Editore: Commonwealth Scientific and Industrial Research Organization Publishing
DOI: 10.1071/RD17551

Subjective and objective assessment of fish sperm motility: when the technique and technicians matter

Autori: V. Gallego, J. G. Herranz-Jusdado, C. Rozenfeld, L. Pérez, Juan F. Asturiano
Pubblicato in: Fish Physiology and Biochemistry, Numero 17 Apr, 2018, Pagina/e 1457–1467, ISSN 0920-1742
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10695-018-0505-1

Tachykinin-3 Genes and Peptides Characterized in a Basal Teleost, the European Eel: Evolutionary Perspective and Pituitary Role

Autori: Aurora Campo, Anne-Gaëlle Lafont, Benjamin Lefranc, Jérôme Leprince, Hervé Tostivint, Nédia Kamech, Sylvie Dufour, Karine Rousseau
Pubblicato in: Frontiers in Endocrinology, Numero 9, 2018, Pagina/e 304, ISSN 1664-2392
Editore: Frontiers
DOI: 10.3389/fendo.2018.00304

Fish Sperm Assessment Using Software and Cooling Devices

Autori: Carina Caldeira, Carles Soler
Pubblicato in: Journal of Visualized Experiments, Numero 137, 2018, ISSN 1940-087X
Editore: MYJoVE Corporation
DOI: 10.3791/56823

Public perception of river fish biodiversity in four European countries

Autori: Sophia Kochalski, Carsten Riepe, Marie Fujitani, Øystein Aas, Robert Arlinghaus
Pubblicato in: Conservation Biology, 2018, ISSN 0888-8892
Editore: Blackwell Publishing Inc.
DOI: 10.1111/cobi.13180

Neurobiology of Wild and Hatchery-Reared Atlantic Salmon: How Nurture Drives Neuroplasticity

Autori: Daan Mes, Kristine von Krogh, Marnix Gorissen, Ian Mayer, Marco A. Vindas
Pubblicato in: Frontiers in Behavioral Neuroscience, Numero 12, 2018, ISSN 1662-5153
Editore: Frontiers Research Foundation
DOI: 10.3389/fnbeh.2018.00210

Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with a CASA-Mot system

Autori: Anthony Valverde, Mónica Madrigal, Carina Caldeira, Daznia Bompart, Javier Núñez de Murga, Sandra Arnau, Carles Soler
Pubblicato in: Reproduction in Domestic Animals, 2018, ISSN 0936-6768
Editore: Blackwell Publishing Inc.
DOI: 10.1111/rda.13320

Comparison of European eel sperm cryopreservation protocols with standardization as a target

Autori: J.G. Herranz-Jusdado, V. Gallego, M. Morini, C. Rozenfeld, L. Pérez, E. Kása, T. Kollár, A. Depincé, C. Labbé, Á. Horváth, J.F. Asturiano
Pubblicato in: Aquaculture, Numero 498, 2019, Pagina/e 539-544, ISSN 0044-8486
Editore: Elsevier BV
DOI: 10.1016/j.aquaculture.2018.09.006

Sperm kinematic characterization of alpaca ( Vicugna pacos L.) during the reproductive season

Autori: Aydee Meza, Carina Caldeira, Anthony Valverde, César Ordóñez, Enrique Ampuero, Hernán Cucho, Carles Soler
Pubblicato in: Reproduction in Domestic Animals, Numero 53/6, 2018, Pagina/e 1415-1423, ISSN 0936-6768
Editore: Blackwell Publishing Inc.
DOI: 10.1111/rda.13284

Critical swimming speed of sterlet ( Acipenser ruthenus ): Does intraspecific hybridization affect swimming performance?

Autori: Sahana Shivaramu, Carlos E. Santo, Vojtěch Kašpar, David Bierbach, Jörn Gessner, Marek Rodina, David Gela, Martin Flajšhans, Sven Wuertz
Pubblicato in: Journal of Applied Ichthyology, 2018, ISSN 0175-8659
Editore: Blackwell Publishing Inc.
DOI: 10.1111/jai.13834

Recombinant vs purified mammal gonadotropins as maturation hormonal treatments of European eel males

Autori: J.G. Herranz-Jusdado, C. Rozenfeld, M. Morini, L. Pérez, J.F. Asturiano, V. Gallego
Pubblicato in: Aquaculture, Numero 501, 2019, Pagina/e 527-536, ISSN 0044-8486
Editore: Elsevier BV
DOI: 10.1016/j.aquaculture.2018.12.015

How to improve foraging efficiency for restocking measures of juvenile Baltic sturgeon (Acipenser oxyrinchus)

Autori: Maria Cámara-Ruiz, Carlos Espirito Santo, Joern Gessner, Sven Wuertz
Pubblicato in: Aquaculture, Numero 502, 2019, Pagina/e 12-17, ISSN 0044-8486
Editore: Elsevier BV
DOI: 10.1016/j.aquaculture.2018.12.021

European eel sperm storage: Optimization of short-term protocols and cryopreservation of large volumes

Autori: J.G. Herranz-Jusdado, V. Gallego, C. Rozenfeld, M. Morini, L. Pérez, J.F. Asturiano
Pubblicato in: Aquaculture, Numero 506, 2019, Pagina/e 42-50, ISSN 0044-8486
Editore: Elsevier BV
DOI: 10.1016/j.aquaculture.2019.03.019

Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii)

Autori: Carina Caldeira, Sandra Hernández-Ibáñez, Anthony Valverde, Patrick Martin, Juan G. Herranz-Jusdado, Víctor Gallego, Juan F. Asturiano, Borys Dzyuba, Martin Pšenička, Carles Soler
Pubblicato in: Aquaculture, Numero 502, 2019, Pagina/e 223-231, ISSN 0044-8486
Editore: Elsevier BV
DOI: 10.1016/j.aquaculture.2018.12.001

Influence of interspecific hybridization on fitness-related traits in Siberian sturgeon and Russian sturgeon

Autori: S. Shivaramu, D.T. Vuong, M. Havelka, H. Šachlová, I. Lebeda, V. Kašpar, M. Flajšhans
Pubblicato in: Czech Journal of Animal Science, Numero 64/No. 2, 2019, Pagina/e 78-88, ISSN 1212-1819
Editore: Ceska Akademie Zemedelskych Ved
DOI: 10.17221/165/2018-cjas

Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis

Autori: Mitchell S. Fleming, Gersende Maugars, Anne-Gaëlle Lafont, Jocelyn Rancon, Romain Fontaine, Rasoul Nourizadeh-Lillabadi, Finn-Arne Weltzien, Elena Santidrian Yebra-Pimentel, Ron Dirks, Stephen D. McCormick, Karine Rousseau, Patrick Martin, Sylvie Dufour
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-40019-5

Deep transcriptome analysis of the heat shock response in an Atlantic sturgeon (Acipenser oxyrinchus) cell line

Autori: Elena Santidrián Yebra-Pimentel, Marina Gebert, Hans J. Jansen, Susanne A. Jong-Raadsen, Ron P.H. Dirks
Pubblicato in: Fish & Shellfish Immunology, Numero 88, 2019, Pagina/e 508-517, ISSN 1050-4648
Editore: Academic Press
DOI: 10.1016/j.fsi.2019.03.014

Sustainability and conflict in small-scale fisheries

Autori: Sophia Kochalski
Pubblicato in: 2017
Editore: University of Liverpool

Effects of the hatchery environment on neurobiology and behaviour in Atlantic salmon: implications for stocking

Autori: Daan Mes
Pubblicato in: 2018, ISBN 978-82-575-1766-3
Editore: Norwegian University of Life Sciences (PhD thesis 2018:80)

Neuroendocrine regulation of pituitary gonadotropins during puberty in Atlantic salmon parr with focus on melatonin and Gnrh systems

Autori: Elia Ciani
Pubblicato in: 2018, ISBN 978-82-575-1770-0
Editore: Norwegian University of Life Sciences (PhD thesis 2018:105)

Cultivating conflict: Perspectives on the human dimensions of voluntary Atlantic salmon (Salmo salar) hatcheries in a conservation context

Autori: Hannah Harrison
Pubblicato in: 2018, ISBN 978-82-575-1544-7
Editore: Norwegian University of Life Sciences (2018:76)

Characterization of tachykinin system and role in reproduction in the European eel

Autori: Aurora Campo
Pubblicato in: 2018
Editore: Museum National d' Historie Naturelle

Environmental and neuroendocrine control of smoltification in long-river (Loire-Allier) Atlantic salmon

Autori: Mitchell S. Fleming
Pubblicato in: 2018
Editore: Museum National d' Historie Naturelle

Endangered Waters: saving Europe’s most iconic fish species

Autori: H Harrison, D Mees, M Fleming, and all IMPRESS students
Pubblicato in: Euroscientist, 2016
Editore: Euroscience

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