Skip to main content

Nanostructured molecular decoders for the quantitative, multiplexed, layer-by-layer detection of disease-associated proteins

Ziel

The long-range goal of this project is to develop a molecular nanodevice, based on nucleic acid-protein conjugates, for the multiplexed, quantitative imaging of biomarkers in tissue samples and cultured cells.
The Immuno-nanodecoder will be used for the accurate molecular characterization of skin cancer (melanoma) and glycogenosis type II cellular models and to evaluate the in vitro response to experimental therapies.
The nanodevices (nanodecoders) will consist of self-assembled DNA nanostructures that can reversibly change their fluorescence signal output in response to hybridization to nucleic acid sequences, or to a specific enzymatic reaction. Each nanodevice will be coupled to a specific molecular probe, such as an antibody, peptide, or protein that uniquely recognize disease biomarkers. The coupling will allow the nanodecoder to detect biomarker presence and distribution in cells and tissues, in a layer-by-layer fashion, using optical fluorescence microscopy.
The number of biomarkers that can be detected will be limited only by the capacity to design nanodecoders with differing specificities, which is essentially unlimited.
The creation of nanodecoders and optimization of their function will greatly advance biomarker imaging, which currently lacks a high-throughput, convenient method for the in situ, quantitative microscopic analysis of altered tissue regions.
This project will be driven by knowledge exchange and the expertise of an interdisciplinary team comprised of both early-stage and experienced university and hospital researchers.
Complementary research programs, ranging from nanotechnology to molecular medicine and pathology will support each step of the developmental and applicative parts of the project towards the achievement of its objectives.
Funding of this program will enable long-term, transformative collaborations that will contribute to the integration and collaboration of research groups between European Countries and key Third Countries.

Koordinator

UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA
Netto-EU-Beitrag
€ 198 000,00
Adresse
Via Cracovia 50
00133 Roma
Italien

Auf der Karte ansehen

Region
Centro (IT) Lazio Roma
Aktivitätstyp
Higher or Secondary Education Establishments
Nicht-EU-Beitrag
€ 0,00

Beteiligte (2)

AZIENDA SANITARIA UNIVERSITARIA FRIULI CENTRALE
Italien
Netto-EU-Beitrag
€ 180 000,00
Adresse
Via Pozzuolo 330
33100 Udine

Auf der Karte ansehen

Region
Nord-Est Friuli-Venezia Giulia Udine
Aktivitätstyp
Research Organisations
Nicht-EU-Beitrag
€ 0,00
UNIVERSITY OF LINCOLN
United Kingdom
Netto-EU-Beitrag
€ 63 000,00
Adresse
Brayford Pool
LN6 7TS Lincoln

Auf der Karte ansehen

Region
East Midlands (England) Lincolnshire Lincolnshire
Aktivitätstyp
Higher or Secondary Education Establishments
Nicht-EU-Beitrag
€ 0,00

Partner (2)

Partner

Partnerorganisationen tragen zur Umsetzung der Aktion bei, unterzeichnen jedoch nicht die Finanzhilfevereinbarung.

Temple University-Of The Commonwealth System of Higher Education
Vereinigte Staaten
Netto-EU-Beitrag
€ 0,00
Adresse
N. Broad Street 1601
19122 Philadelphia

Auf der Karte ansehen

Aktivitätstyp
Higher or Secondary Education Establishments
Nicht-EU-Beitrag
€ 0,00
Partner

Partnerorganisationen tragen zur Umsetzung der Aktion bei, unterzeichnen jedoch nicht die Finanzhilfevereinbarung.

CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
Argentinien
Netto-EU-Beitrag
€ 0,00
Nicht-EU-Beitrag
€ 139 500,00