Structure of paramagnetic integral membrane metalloproteins by MAS-NMR

Project Information

P-MEM-NMR

Grant agreement ID: 648974

DOI 10.3030/648974

Project closed

EC signature date 6 July 2015

Start date 1 September 2015

End date 31 August 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 499 375,00

EU contribution € 2 499 375,00

Coordinated by

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (30)

Probing Membrane Protein Insertion into Lipid Bilayers by Solid‐State NMR

Author(s): Eszter E. Najbauer, Kumar Tekwani Movellan, Tobias Schubeis, Tom Schwarzer, Kathrin Castiglione, Karin Giller, Guido Pintacuda, Stefan Becker, Loren B. Andreas
Published in: ChemPhysChem, 2018, ISSN 1439-4235
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cphc.201800793

Multimodal Response to Copper Binding in Superoxide Dismutase Dynamics

Author(s): Marta Bonaccorsi, Michael J. Knight, Tanguy Le Marchand, Hugh R. W. Dannatt, Tobias Schubeis, Loïc Salmon, Isabella C. Felli, Lyndon Emsley, Roberta Pierattelli, Guido Pintacuda
Published in: Journal of the American Chemical Society, Issue 142/46, 2020, Page(s) 19660-19667, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c09242

Backbone assignment of crystalline E. coli maltose binding protein

Author(s): Tobias Schubeis, Jan Stanek, Guido Pintacuda
Published in: Biomolecular NMR Assignments, Issue 15/2, 2021, Page(s) 317-322, ISSN 1874-2718
Publisher: Springer Verlag
DOI: 10.1007/s12104-021-10023-w

Structural and molecular basis of cross-seeding barriers in amyloids

Author(s): Asen Daskalov, Denis Martinez, Virginie Coustou, Nadia El Mammeri, Mélanie Berbon, Loren B. Andreas, Benjamin Bardiaux, Jan Stanek, Abdelmajid Noubhani, Brice Kauffmann, Joseph S. Wall, Guido Pintacuda, Sven J. Saupe, Birgit Habenstein, Antoine Loquet
Published in: Proceedings of the National Academy of Sciences, Issue 118/1, 2020, Page(s) e2014085118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2014085118

A β-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL

Author(s): Tobias Schubeis, Tanguy Le Marchand, Csaba Daday, Wojciech Kopec, Kumar Tekwani Movellan, Jan Stanek, Tom S. Schwarzer, Kathrin Castiglione, Bert L. de Groot, Guido Pintacuda, Loren B. Andreas
Published in: Proceedings of the National Academy of Sciences, Issue 117/35, 2020, Page(s) 21014-21021, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2002598117

Paramagnetic NMR in solution and the solid state

Author(s): Andrew J. Pell, Guido Pintacuda, Clare P. Grey
Published in: Progress in Nuclear Magnetic Resonance Spectroscopy, Issue 111, 2019, Page(s) 1-271, ISSN 0079-6565
Publisher: Elsevier BV
DOI: 10.1016/j.pnmrs.2018.05.001

Picometer Resolution Structure of the Coordination Sphere in the Metal-Binding Site in a Metalloprotein by NMR

Author(s): Andrea Bertarello, Ladislav Benda, Kevin J. Sanders, Andrew J. Pell, Michael J. Knight, Vladimir Pelmenschikov, Leonardo Gonnelli, Isabella C. Felli, Martin Kaupp, Lyndon Emsley, Roberta Pierattelli, Guido Pintacuda
Published in: Journal of the American Chemical Society, Issue 142/39, 2020, Page(s) 16757-16765, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c07339

Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR

Author(s): Tobias Schubeis, Tom S. Schwarzer, Tanguy Le Marchand, Jan Stanek, Kumar Tekwani Movellan, Kathrin Castiglione, Guido Pintacuda, Loren B. Andreas
Published in: Biomolecular NMR Assignments, Issue 14/2, 2020, Page(s) 295-300, ISSN 1874-2718
Publisher: Springer Verlag
DOI: 10.1007/s12104-020-09964-5

Proton-detected fast-magic-angle spinning NMR of paramagnetic inorganic solids

Author(s): Jan Blahut, Ladislav Benda, Arthur L. Lejeune, Kevin J. Sanders, Benjamin Burcher, Erwann Jeanneau, David Proriol, Leonor Catita, Pierre-Alain R. Breuil, Anne-Agathe Quoineaud, Andrew J. Pell, Guido Pintacuda
Published in: RSC Advances, Issue 11/47, 2021, Page(s) 29870-29876, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1ra04110j

Protein structural dynamics by Magic-Angle Spinning NMR

Author(s): Marta Bonaccorsi, Tanguy Le Marchand, Guido Pintacuda
Published in: Current Opinion in Structural Biology, Issue 70, 2021, Page(s) 34-43, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2021.02.008

Monitoring Dynamics, Structure, and Magnetism of Switchable Metal–Organic Frameworks via 1 H‐Detected MAS NMR

Author(s): Jan Blahut, Arthur L. Lejeune, Sebastian Ehrling, Irena Senkovska, Stefan Kaskel, Florian M. Wisser, Guido Pintacuda
Published in: Angewandte Chemie International Edition, Issue 60/40, 2021, Page(s) 21778-21783, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202107032

Structural Analysis of an Antigen Chemically Coupled on Virus‐Like Particles in Vaccine Formulation

Author(s): Kristaps Jaudzems, Anna Kirsteina, Tobias Schubeis, Gilles Casano, Olivier Ouari, Janis Bogans, Andris Kazaks, Kaspars Tars, Anne Lesage, Guido Pintacuda
Published in: Angewandte Chemie International Edition, Issue 60/23, 2021, Page(s) 12847-12851, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202013189

Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid‐State Automated Projection SpectroscopY (SO‐APSY)

Author(s): Henry W. Orton, Jan Stanek, Tobias Schubeis, Dylan Foucaudeau, Claire Ollier, Adrian W. Draney, Tanguy Le Marchand, Diane Cala‐De Paepe, Isabella C. Felli, Roberta Pierattelli, Sebastian Hiller, Wolfgang Bermel, Guido Pintacuda
Published in: Angewandte Chemie International Edition, Issue 59/6, 2020, Page(s) 2380-2384, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201912211

Impact of Magnetic Field Strength on Resolution and Sensitivity of Proton Resonances in Biological Solids

Author(s): Kai Xue, Riddhiman Sarkar, Daniela Lalli, Benita Koch, Guido Pintacuda, Zdenek Tosner, Bernd Reif
Published in: The Journal of Physical Chemistry C, Issue 124/41, 2020, Page(s) 22631-22637, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.0c05407

Proton-Based Structural Analysis of a Heptahelical Transmembrane Protein in Lipid Bilayers

Author(s): Daniela Lalli, Matthew N. Idso, Loren B. Andreas, Sunyia Hussain, Naomi Baxter, Songi Han, Bradley F. Chmelka, Guido Pintacuda
Published in: Journal of the American Chemical Society, Issue 139/37, 2017, Page(s) 13006-13012, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b05269

Selective 1 H– 1 H Distance Restraints in Fully Protonated Proteins by Very Fast Magic-Angle Spinning Solid-State NMR

Author(s): Mukul G. Jain, Daniela Lalli, Jan Stanek, Chandrakala Gowda, Satya Prakash, Tom S. Schwarzer, Tobias Schubeis, Kathrin Castiglione, Loren B. Andreas, P. K. Madhu, Guido Pintacuda, Vipin Agarwal
Published in: The Journal of Physical Chemistry Letters, Issue 8/11, 2017, Page(s) 2399-2405, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.7b00983

Paramagnetic Properties of a Crystalline Iron–Sulfur Protein by Magic-Angle Spinning NMR Spectroscopy

Author(s): Andrea Bertarello, Tobias Schubeis, Carmelo Fuccio, Enrico Ravera, Marco Fragai, Giacomo Parigi, Lyndon Emsley, Guido Pintacuda, Claudio Luchinat
Published in: Inorganic Chemistry, Issue 56/11, 2017, Page(s) 6624-6629, ISSN 0020-1669
Publisher: American Chemical Society
DOI: 10.1021/acs.inorgchem.7b00674

NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils

Author(s): Jan Stanek, Loren B. Andreas, Kristaps Jaudzems, Diane Cala, Daniela Lalli, Andrea Bertarello, Tobias Schubeis, Inara Akopjana, Svetlana Kotelovica, Kaspars Tars, Andrea Pica, Serena Leone, Delia Picone, Zhi-Qiang Xu, Nicholas E. Dixon, Denis Martinez, Mélanie Berbon, Nadia El Mammeri, Abdelmajid Noubhani, Sven Saupe, Birgit Habenstein, Antoine Loquet, Guido Pintacuda
Published in: Angewandte Chemie International Edition, Issue 55/50, 2016, Page(s) 15504-15509, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201607084

Structure of fully protonated proteins by proton-detected magic-angle spinning NMR

Author(s): Loren B. Andreas, Kristaps Jaudzems, Jan Stanek, Daniela Lalli, Andrea Bertarello, Tanguy Le Marchand, Diane Cala-De Paepe, Svetlana Kotelovica, Inara Akopjana, Benno Knott, Sebastian Wegner, Frank Engelke, Anne Lesage, Lyndon Emsley, Kaspars Tars, Torsten Herrmann, Guido Pintacuda
Published in: Proceedings of the National Academy of Sciences, Issue 113/33, 2016, Page(s) 9187-9192, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1602248113

1 H magic-angle spinning NMR evolves as a powerful new tool for membrane proteins

Author(s): Tobias Schubeis, Tanguy Le Marchand, Loren B. Andreas, Guido Pintacuda
Published in: Journal of Magnetic Resonance, Issue 287, 2018, Page(s) 140-152, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2017.11.014

Low-power broadband solid-state MAS NMR of 14 N

Author(s): Andrew J. Pell, Kevin J. Sanders, Sebastian Wegner, Guido Pintacuda, Clare P. Grey
Published in: The Journal of Chemical Physics, Issue 146/19, 2017, Page(s) 194202, ISSN 0021-9606
Publisher: American Institute of Physics
DOI: 10.1063/1.4983220

Conformational dynamics in crystals reveal the molecular bases for D76N beta-2 microglobulin aggregation propensity

Author(s): Tanguy Le Marchand, Matteo de Rosa, Nicola Salvi, Benedetta Maria Sala, Loren B. Andreas, Emeline Barbet-Massin, Pietro Sormanni, Alberto Barbiroli, Riccardo Porcari, Cristiano Sousa Mota, Daniele de Sanctis, Martino Bolognesi, Lyndon Emsley, Vittorio Bellotti, Martin Blackledge, Carlo Camilloni, Guido Pintacuda, Stefano Ricagno
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04078-y

Is protein deuteration beneficial for proton detected solid-state NMR at and above 100 kHz magic-angle spinning?

Author(s): Diane Cala-De Paepe, Jan Stanek, Kristaps Jaudzems, Kaspars Tars, Loren B. Andreas, Guido Pintacuda
Published in: Solid State Nuclear Magnetic Resonance, Issue 87, 2017, Page(s) 126-136, ISSN 0926-2040
Publisher: Academic Press
DOI: 10.1016/j.ssnmr.2017.07.004

Straightforward Access to Stable, 16-Valence-Electron Phosphine-Stabilized Fe 0 Olefin Complexes and Their Reactivity

Author(s): Benjamin Burcher, Kevin J. Sanders, Ladislav Benda, Guido Pintacuda, Erwann Jeanneau, Andreas A. Danopoulos, Pierre Braunstein, Hélène Olivier-Bourbigou, Pierre-Alain R. Breuil
Published in: Organometallics, Issue 36/3, 2017, Page(s) 605-613, ISSN 0276-7333
Publisher: American Chemical Society
DOI: 10.1021/acs.organomet.6b00803

Local and Global Dynamics in Klebsiella pneumoniae Outer Membrane Protein a in Lipid Bilayers Probed at Atomic Resolution

Author(s): Olivier Saurel, Iordan Iordanov, Guillaume Nars, Pascal Demange, Tanguy Le Marchand, Loren B. Andreas, Guido Pintacuda, Alain Milon
Published in: Journal of the American Chemical Society, Issue 139/4, 2017, Page(s) 1590-1597, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.6b11565

Broadband MAS NMR spectroscopy in the low-power limit

Author(s): Kevin J. Sanders, Andrew J. Pell, Sebastian Wegner, Clare P. Grey, Guido Pintacuda
Published in: Chemical Physics Letters, Issue 697, 2018, Page(s) 29-37, ISSN 0009-2614
Publisher: Elsevier BV
DOI: 10.1016/j.cplett.2018.01.028

3D structure determination of amyloid fibrils using solid-state NMR spectroscopy

Author(s): Antoine Loquet, Nadia El Mammeri, Jan Stanek, Mélanie Berbon, Benjamin Bardiaux, Guido Pintacuda, Birgit Habenstein
Published in: Methods, 2018, ISSN 1046-2023
Publisher: Academic Press
DOI: 10.1016/j.ymeth.2018.03.014

Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Author(s): Alexander Marchanka, Jan Stanek, Guido Pintacuda, Teresa Carlomagno
Published in: Chemical Communications, Issue 54/65, 2018, Page(s) 8972-8975, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc04437f

MAS dependent sensitivity of different isotopomers in selectively methyl protonated protein samples in solid state NMR

Author(s): Kai Xue, Riddhiman Sarkar, Zdenek Tosner, Daniela Lalli, Carina Motz, Benita Koch, Guido Pintacuda, Bernd Reif
Published in: Journal of Biomolecular NMR, Issue 73/10-11, 2019, Page(s) 625-631, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-019-00274-0

Automated Backbone NMR Resonance Assignment of Large Proteins Using Redundant Linking from a Single Simultaneous Acquisition

Author(s): Jan Stanek, Tobias Schubeis, Piotr Paluch, Peter Güntert, Loren B. Andreas, Guido Pintacuda
Published in: Journal of the American Chemical Society, Issue 142/12, 2020, Page(s) 5793-5799, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c00251

Non-peer reviewed articles (1)

Mg2+-dependent conformational equilibria in CorA: an integrated view on transport regulation

Author(s): Nicolai Tidemand Johansen, Marta Bonaccorsi, Tone Bengtsen, Andreas Haahr Larsen, Frederik Grønbæk Tidemand, Martin Cramer Pedersen, Pie Huda, Jens Berndtsson, Tamim Darwish, Nageshwar Rao Yepuri, Anne Martel, Thomas Günther Pomorski, Andrea Bertarello, Mark Sansom, Mikaela Rapp, Ramon Crehuet, Tobias Schubeis, Kresten Lindorff-Larsen, Guido Pintacuda, Lise Arleth
Published in: bioRxiv, 2021, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.08.20.457080