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Controlled cryo-EM sample preparation through DNA-Origami pillars

Obiettivo

Electron cryomicroscopy (cryo-EM) is rapidly emerging as a powerful technique for high-resolution structure determination of protein complexes. Despite recent advances, there is still ample room for improvement of this method, in particular regarding sample preparation. Outstanding challenges are reproducibility of freezing conditions to obtain an optimal ice thickness; to prevent unfolding or preferential orientation of proteins against the hydrophobic air-water interface; and a limited accuracy of beam-induced motion correction for relatively small particles. Here, we propose to study how DNA origami objects that are added in with the sample may affect cryo-EM grid preparation. In addition, by examining a variety of DNA origami structures in the electron microscope, we also aim to identify and improve on structural weaknesses in their design. We will test new design strategies, analyse those, and use them for application in cryo-EM. Thereby, this proposal will facilitate both cryo-EM structure determination and the design of nano-tools that are made from DNA.

Meccanismo di finanziamento

MSCA-IF-EF-ST - Standard EF

Coordinatore

UNITED KINGDOM RESEARCH AND INNOVATION
Contribution nette de l'UE
€ 183 454,80
Indirizzo
POLARIS HOUSE NORTH STAR AVENUE
SN2 1FL Swindon
Regno Unito

Mostra sulla mappa

Regione
South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Swindon
Tipo di attività
Research Organisations
Collegamenti
Costo totale
€ 183 454,80