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New generation of carbohydrate-based vaccines via rational understanding of their immunological mechanism

Obiettivo

Glycoconjugate vaccines have provided enormous health benefits globally, but they have been less successful in some populations at high risk for developing disease. They are composed by a sugar antigen covalently linked to a carrier protein. The traditional hypothesis of immune activation by glycoconjugate vaccines suggests that only peptides generated from glycoconjugate processing can be presented to and recognized by T cells, and this contribution is crucial for their immunogenicity. In most cases, conjugation processes have been set-up empirically.
Recently, new findings offer a rational explanation for how conjugates work and may render vaccine development a more straightforward process. In contrast with the classical mechanism, this new model suggests that carbohydrate presentation to the T cell by antigen-presenting cell may strongly enhance antibody response. The key
strategy is to conjugate the carbohydrate to peptides which anchor the conjugate via MHC class II and allow the sugar epitope to be presented via the T cell receptor. Application of this principle resulted in a GBSIII vaccine strongly protective in a mouse model and 50–100 times more immunogenic than a traditional vaccine composed by
random linking of the sugar on a protein carrier. Although the principle has been demonstrated much remains to be done to generally apply the concept to generate vaccines for clinical use. In the proposed study, we will extend the approach by analysing different variables (peptide carrier, glycan chain length, conjugation chemistry and microbial antigen), with the aim of using the increased understanding of basic immunological mechanisms to develop a new translational platform for optimized and cost-effective carbohydrate-based vaccines. Innovative strategies of conjugation chemistry will be also evaluated to generate new therapeutics with chemical properties designed in light of specific information on antigen presentation.

Invito a presentare proposte

H2020-MSCA-IF-2014
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Meccanismo di finanziamento

MSCA-IF-GF - Global Fellowships

Coordinatore

UNIVERSITA DEGLI STUDI DI MILANO
Indirizzo
Via Festa Del Perdono 7
20122 Milano
Italia
Tipo di attività
Higher or Secondary Education Establishments
Contributo UE
€ 244 269

Partecipanti (1)

GlaxoSmithKline Vaccines Srl

Partecipazione conclusa

Italia
Contributo UE
€ 0
Indirizzo
Via Fiorentina
53100 Siena
Tipo di attività
Private for-profit entities (excluding Higher or Secondary Education Establishments)

Partner (1)

PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Stati Uniti
Indirizzo
Massachusetts Avenue 1350
02138 Cambridge
Tipo di attività
Higher or Secondary Education Establishments