Livrables Other (2) Dissemination event with patient groups The consortium will organize a patient and families dissemination event which will be communicating results as well as receiving input from patients and their families. The event will be organised in collaboration with the three patient support groups associated to the project Tool for engaging the public A new tool for engaging publics, including school students and patients, around short bowel syndrome, and potential clinical uses of intestinal stem cells. Documents, reports (8) SOP for the cell culture methods A Standard operating procedure for obtaining expanding and seeding of cells on a scaffold for the purpose of transplantation Analysis of TEI in mouse We will deliver a histological, immunohistochemical and morphometric description of implanted TEI in the mouse. Guidelines for quality assessment of intestinal cell lineages utilised for transplantation The consortium members will draft an open letter to be published, which describes the minimum requirement for quality assessment of intestinal cell lineages if they are to be implemented for therapeutic transplantation. Obtaining tissue We will set up a standard Operating Procedure for obtaining the tissue sample that is required for the in vitro procedure up to the transplantation. The protocol will describe the regulatory approval, the logistics and the technical description of the isolation up to taking it into culture. Outcome and function of TEI in piglets Survival and weight gain of piglets with TEI implanted in the omentum will be described TEI will be characterized morphometrically and histologically and the function of the TEI also characterized with comparison between iPS and postnatal stem cell based implants Report on health economics related to INTENS objectives Produce a report based on information gathering from leading social scientists whose research already addresses these areas and make their findings available in the public domain Report on dissemination of project outcomes, patient engagement and mobility program A report summarizing the dissemination of project outcome, patient engagement and mobility program. Will include lessons learned and suggested improvements for future projects. Outcome and function of small bowel mucosa in large bowel in piglets Survival and weight gain of piglets with engineered small bowel mucosa implanted in the large bowel in substitution of colonic mucosa will be described Function will be characterized and engineered mucosa characterized morphometrically and histologically with comparison between iPS and postnatal stem cell based implants Websites, patent fillings, videos etc. (1) Website Creation and launch of INTENS project-specific website at www.intens.info as well as linking from EuroStemCell, PINNT, Short Gut Foundation and other relevant websites. Publications Peer reviewed articles (30) Homeostatic mini-intestines through scaffold-guided organoid morphogenesis Auteurs: Mikhail Nikolaev, Olga Mitrofanova, Nicolas Broguiere, Sara Geraldo, Devanjali Dutta, Yoji Tabata, Bilge Elci, Nathalie Brandenberg, Irina Kolotuev, Nikolce Gjorevski, Hans Clevers, Matthias P. Lutolf Publié dans: Nature, Issue 585/7826, 2020, Page(s) 574-578, ISSN 0028-0836 Éditeur: Nature Publishing Group DOI: 10.1038/s41586-020-2724-8 NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor Auteurs: Laura Novellasdemunt, Anna Kucharska, Cara Jamieson, Maria Prange‐Barczynska, Anna Baulies, Pedro Antas, Jelte van der Vaart, Helmuth Gehart, Madelon M Maurice, Vivian SW Li Publié dans: The EMBO Journal, Issue 39/3, 2019, ISSN 0261-4189 Éditeur: Nature Publishing Group DOI: 10.15252/embj.2019102771 Building gut from scratch — progress and update of intestinal tissue engineering Auteurs: Tullie L, Jones BC, De Coppi P, Li VSW Publié dans: Nature Reviews Gastroenterology and Hepatology, Issue 19, 2022, Page(s) 417-431, ISSN 1759-5045 Éditeur: Nature Publishing Group DOI: 10.1038/s41575-022-00586-x Tissue-Engineering the Intestine: The Trials before the Trials Auteurs: Hans Clevers, Ryan K. Conder, Vivian S.W. Li, Matthias P. Lutolf, Ludovic Vallier, Sarah Chan, Tracy C. Grikscheit, Kim B. Jensen, Paolo De Coppi Publié dans: Cell Stem Cell, Issue 24/6, 2019, Page(s) 855-859, ISSN 1934-5909 Éditeur: Cell Press DOI: 10.1016/j.stem.2019.04.018 Bioengineering human intestinal mucosal grafts using patient derived organoids, fibroblasts and scaffolds Auteurs: Meran L, Tullie L, Eaton S, De Coppi P, Li V Publié dans: Nature Protocols, Issue 18, 2023, Page(s) 108-135, ISSN 1750-2799 Éditeur: Springer Nature DOI: 10.1038/s41596-022-00751-1 Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture Auteurs: Giovanni Giuseppe Giobbe, Claire Crowley, Camilla Luni, Sara Campinoti, Moustafa Khedr, Kai Kretzschmar, Martina Maria De Santis, Elisa Zambaiti, Federica Michielin, Laween Meran, Qianjiang Hu, Gijs van Son, Luca Urbani, Anna Manfredi, Monica Giomo, Simon Eaton, Davide Cacchiarelli, Vivian S. W. Li, Hans Clevers, Paola Bonfanti, Nicola Elvassore, Paolo De Coppi Publié dans: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723 Éditeur: Nature Publishing Group DOI: 10.1038/s41467-019-13605-4 Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation Auteurs: Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic Publié dans: Genome Biology, Issue 21/1, 2020, ISSN 1474-760X Éditeur: Springer Nature DOI: 10.1186/s13059-020-02058-4 Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling Auteurs: "Martti Maimets1,2,3 #, Marianne Terndrup Pedersen1,2, #, Jordi Guiu1,2, Jes Dreier2,4, Malte Thodberg1,2,5, Yasuko Antoku1, Pawel J. Schweiger1,2, Leonor Rib1, Raul Bardini Bressan1,2,3, Yi Miao6, K. Christopher Garcia6,7, Albin Sandelin1,5, Palle Serup2,3 and Kim B. Jensen1,2,3*" Publié dans: Nature Communications, Issue Vol 13 (article no. 715), 2022, ISSN 2041-1723 Éditeur: Nature Publishing Group DOI: 10.1038/s41467-022-28369-7 Genome-Wide Epigenetic and Transcriptomic Characterization of Human-Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Organoids Auteurs: Judith Kraiczy, Alexander D.B. Ross, Jessica L. Forbester, Gordon Dougan, Ludovic Vallier, Matthias Zilbauer Publié dans: Cellular and Molecular Gastroenterology and Hepatology, Issue 7/2, 2019, Page(s) 285-288, ISSN 2352-345X Éditeur: Elsevier Inc. DOI: 10.1016/j.jcmgh.2018.10.008 The Transcription Co-Repressors MTG8 and MTG16 Regulate Exit of Intestinal Stem Cells From Their Niche and Differentiation Into Enterocyte vs Secretory Lineages Auteurs: Anna Baulies, Nikolaos Angelis, Valentina Foglizzo, E. Thomas Danielsen, Harshil Patel, Laura Novellasdemunt, Anna Kucharska, Joana Carvalho, Emma Nye, Paolo De Coppi, Vivian S.W. Li Publié dans: Gastroenterology, 2020, ISSN 0016-5085 Éditeur: W. B. Saunders Co., Ltd. DOI: 10.1053/j.gastro.2020.06.012 Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn’s Disease Auteurs: Rasa Elmentaite et al Publié dans: Developmental Cell, Issue 00222593, 2020, ISSN 0022-2593 Éditeur: British Medical Association DOI: 10.1016/j.devcel.2020.11.010 A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs Auteurs: Sassi, L; Ajayi, O; Campinoti, S; Natarajan, D; McQuitty, C; Siena, RR; Mantero, S; De Coppi, P; Pellegata, AF; Chokshi, S; Urbani, L Publié dans: Nanomaterials, Issue 20794991, 2021, ISSN 2079-4991 Éditeur: MDPI DOI: 10.3390/nano11020275 SARS-CoV-2 infection and replication in human gastric organoids. Auteurs: Giovanni Giuseppe Giobbe; Francesco Bonfante; Brendan C. Jones; Onelia Gagliano; Camilla Luni; Elisa Zambaiti; Elisa Zambaiti; Silvia Perin; Cecilia Laterza; Georg A. Busslinger; Hannah T. Stuart; Matteo Pagliari; Alessio Bortolami; Eva Mazzetto; Anna Manfredi; Chiara Colantuono; Lucio Di Filippo; Alessandro Filippo Pellegata; Valentina Panzarin; Nikhil Thapar; Vivian S. W. Li; Simon Eaton; Davide Publié dans: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021), Issue 20411723, 2021, ISSN 2041-1723 Éditeur: Nature Publishing Group DOI: 10.1038/s41467-021-26762-2 Tracing the origin of adult intestinal stem cells Auteurs: Jordi Guiu, Edouard Hannezo, Shiro Yui, Samuel Demharter, Svetlana Ulyanchenko, Martti Maimets, Anne Jørgensen, Signe Perlman, Lene Lundvall, Linn Salto Mamsen, Agnete Larsen, Rasmus H. Olesen, Claus Yding Andersen, Lea Langhoff Thuesen, Kristine Juul Hare, Tune H. Pers, Konstantin Khodosevich, Benjamin D. Simons, Kim B. Jensen Publié dans: Nature, Issue 570/7759, 2019, Page(s) 107-111, ISSN 0028-0836 Éditeur: Nature Publishing Group DOI: 10.1038/s41586-019-1212-5 The hope and the hype of organoid research Auteurs: Meritxell Huch, Juergen A. Knoblich, Matthias P. Lutolf, Alfonso Martinez-Arias Publié dans: Development, Issue 144/6, 2017, Page(s) 938-941, ISSN 0950-1991 Éditeur: The Company of Biologists Ltd. DOI: 10.1242/dev.150201 Multiscale microenvironmental perturbation of pluripotent stem cell fate and self-organization Auteurs: Yoji Tabata, Matthias P. Lutolf Publié dans: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322 Éditeur: Nature Publishing Group DOI: 10.1038/srep44711 Progress and potential in organoid research Auteurs: Giuliana Rossi, Andrea Manfrin, Matthias P. Lutolf Publié dans: Nature Reviews Genetics, Issue 19/11, 2018, Page(s) 671-687, ISSN 1471-0056 Éditeur: Nature Publishing Group DOI: 10.1038/s41576-018-0051-9 YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Auteurs: Shiro Yui, Luca Azzolin, Martti Maimets, Marianne Terndrup Pedersen, Robert P. Fordham, Stine L. Hansen, Hjalte L. Larsen, Jordi Guiu, Mariana R.P. Alves, Carsten F. Rundsten, Jens V. Johansen, Yuan Li, Chris D. Madsen, Tetsuya Nakamura, Mamoru Watanabe, Ole H. Nielsen, Pawel J. Schweiger, Stefano Piccolo, Kim B. Jensen Publié dans: Cell Stem Cell, Issue 22/1, 2018, Page(s) 35-49.e7, ISSN 1934-5909 Éditeur: Cell Press DOI: 10.1016/j.stem.2017.11.001 Mammalian body plan engineering: Lessons and challenges Auteurs: Alfonso Martinez Arias, Matthias P. Lutolf Publié dans: Current Opinion in Systems Biology, Issue 11, 2018, Page(s) 50-56, ISSN 2452-3100 Éditeur: Elsevier DOI: 10.1016/j.coisb.2018.07.007 Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon Auteurs: Conor J. McCann, Julie E. Cooper, Dipa Natarajan, Benjamin Jevans, Laura E. Burnett, Alan J. Burns, Nikhil Thapar Publié dans: Nature Communications, Issue 8, 2017, Page(s) 15937, ISSN 2041-1723 Éditeur: Nature Publishing Group DOI: 10.1038/ncomms15937 Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling Auteurs: Claudia Y. Janda, Luke T. Dang, Changjiang You, Junlei Chang, Wim de Lau, Zhendong A. Zhong, Kelley S. Yan, Owen Marecic, Dirk Siepe, Xingnan Li, James D. Moody, Bart O. Williams, Hans Clevers, Jacob Piehler, David Baker, Calvin J. Kuo, K. Christopher Garcia Publié dans: Nature, Issue 545/7653, 2017, Page(s) 234-237, ISSN 0028-0836 Éditeur: Nature Publishing Group DOI: 10.1038/nature22306 Establishing Proximal and Distal Regional Identities in Murine and Human Tissue-Engineered Lung and Trachea Auteurs: Andrew Trecartin, Soula Danopoulos, Ryan Spurrier, Hanaa Knaneh-Monem, Michael Hiatt, Barbara Driscoll, Christian Hochstim, Denise Al-Alam, Tracy C. Grikscheit Publié dans: Tissue Engineering Part C: Methods, Issue 22/11, 2016, Page(s) 1049-1057, ISSN 1937-3384 Éditeur: Mary Ann Liebert Inc. DOI: 10.1089/ten.TEC.2016.0261 Functional Human and Murine Tissue-Engineered Liver Is Generated from Adult Stem/Progenitor Cells Auteurs: Nirmala Mavila, Andrew Trecartin, Ryan Spurrier, Yi Xiao, Xiaogang Hou, David James, Xiaowei Fu, Brian Truong, Clara Wang, Gerald S. Lipshutz, Kasper S. Wang, Tracy C. Grikscheit Publié dans: STEM CELLS Translational Medicine, Issue 6/1, 2017, Page(s) 238-248, ISSN 2157-6564 Éditeur: AlphaMed Press DOI: 10.5966/sctm.2016-0205 DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development Auteurs: Judith Kraiczy, Komal M Nayak, Kate J Howell, Alexander Ross, Jessica Forbester, Camilla Salvestrini, Roxana Mustata, Sally Perkins, Amanda Andersson-Rolf, Esther Leenen, Anke Liebert, Ludovic Vallier, Philip C Rosenstiel, Oliver Stegle, Gordon Dougan, Robert Heuschkel, Bon-Kyoung Koo, Matthias Zilbauer Publié dans: Gut, Issue 68/1, 2018, Page(s) 49-61, ISSN 0017-5749 Éditeur: BMJ Publishing Group DOI: 10.1136/gutjnl-2017-314817 Low‐Defect Thiol‐Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation Auteurs: Saba Rezakhani, Nikolce Gjorevski, Matthias P. Lutolf Publié dans: Advanced Functional Materials, 2020, Page(s) 2000761, ISSN 1616-301X Éditeur: John Wiley & Sons Ltd. DOI: 10.1002/adfm.202000761 Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation Auteurs: GW-He, L Lin, J DeMartino , X Zheng, N Staliarova, T Dayton, H Begthel, WJ van de Wetering , E Bodewes, J van Zon, S Tans, C Lopez-Iglesias, PJ Peters, W Wu, D Kotlarz, C Klein, T Margaritis, F Holstege, H Clevers Publié dans: Cell Stem Cell. 2022, Issue 29(9), 2022, Page(s) 1333-1345, ISSN 1875-9777 Éditeur: Elsevier DOI: 10.1016/j.stem.2022.08.002 Paediatric gastric organoids as a tool for disease modelling and clinical translation Auteurs: Brendan C. Jones, Giuseppe Calà, Paolo De Coppi & Giovanni Giuseppe Giobbe Publié dans: Pediatric Surgery International, Issue 01790358, 2021, ISSN 0179-0358 Éditeur: Springer Verlag DOI: 10.1007/s00383-020-04821-x SH3BP4 Regulates Intestinal Stem Cells and Tumorigenesis by Modulating β-Catenin Nuclear Localization Auteurs: Pedro Antas, Laura Novellasdemunt, Anna Kucharska, Isobel Massie, Joana Carvalho, Dahmane Oukrif, Emma Nye, Marco Novelli, Vivian S.W. Li Publié dans: Cell Reports, Issue 26/9, 2019, Page(s) 2266-2273.e4, ISSN 2211-1247 Éditeur: Cell Press DOI: 10.1016/j.celrep.2019.01.110 Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis Auteurs: Palikuqi, B; Nguyen, DHT; Li, G; Schreiner, R; Pellegata, AF; Liu, Y; Redmond, D; Geng, FQ; Lin, Y; Gomez-Salinero, JM; Yokoyama, M; Zumbo, P; Zhang, T; Kunar, B; Witherspoon, M; Han, T; Tedeschi, AM; Scottoni, F; Lipkin, SM; Dow, L; Elemento, O; Xiang, JZ; Shido, K; Spence, JR; Zhou, QJ; Schwartz, RE; De Coppi, P; Rabbany, SY; Rafii, S Publié dans: Nature, Issue 14764687, 2020, ISSN 1476-4687 Éditeur: Nature Research DOI: 10.1038/s41586-020-2712-z A Novel Human Pluripotent Stem Cell-Derived Neural Crest Model of Treacher Collins Syndrome Shows Defects in Cell Death and Migration Auteurs: Felipe Serrano, William George Bernard, Alessandra Granata, Dharini Iyer, Ben Steventon, Matthew Kim, Ludovic Vallier, Laure Gambardella, Sanjay Sinha Publié dans: Stem Cells and Development, Issue 28/2, 2019, Page(s) 81-100, ISSN 1547-3287 Éditeur: Mary Ann Liebert Inc. DOI: 10.1089/scd.2017.0234 Non-peer reviewed articles (1) Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure Auteurs: Laween Meran, Isobel Massie, Sara Campinoti, Anne E. Weston, Riana Gaifulina, Lucinda Tullie, Peter Faull, Michael Orford, Anna Kucharska, Anna Baulies, Laura Novellasdemunt, Nikolaos Angelis, Elizabeth Hirst, Julia König, Alfonso Maria Tedeschi, Alessandro Filippo Pellegata, Susanna Eli, Ambrosius P. Snijders, Lucy Collinson, Nikhil Thapar, Geraint M. H. Thomas, Simon Eaton, Paola Bonfanti, Paol Publié dans: Nature Medicine, 2019, ISSN 1078-8956 Éditeur: Nature Publishing Group DOI: 10.1038/s41591-020-1024-z Recherche de données OpenAIRE... Une erreur s’est produite lors de la recherche de données OpenAIRE Aucun résultat disponible
