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INtestinal Tissue ENgineering Solution

Rezultaty

Dissemination event with patient groups

The consortium will organize a patient and families dissemination event which will be communicating results as well as receiving input from patients and their families. The event will be organised in collaboration with the three patient support groups associated to the project

Tool for engaging the public

A new tool for engaging publics, including school students and patients, around short bowel syndrome, and potential clinical uses of intestinal stem cells.

Analysis of TEI in mouse

We will deliver a histological, immunohistochemical and morphometric description of implanted TEI in the mouse.

Guidelines for quality assessment of intestinal cell lineages utilised for transplantation

The consortium members will draft an open letter to be published, which describes the minimum requirement for quality assessment of intestinal cell lineages if they are to be implemented for therapeutic transplantation.

Obtaining tissue

We will set up a standard Operating Procedure for obtaining the tissue sample that is required for the in vitro procedure up to the transplantation. The protocol will describe the regulatory approval, the logistics and the technical description of the isolation up to taking it into culture.

Report on dissemination of project outcomes, patient engagement and mobility program

A report summarizing the dissemination of project outcome, patient engagement and mobility program. Will include lessons learned and suggested improvements for future projects.

Website

Creation and launch of INTENS project-specific website at www.intens.info as well as linking from EuroStemCell, PINNT, Short Gut Foundation and other relevant websites.

Wyszukiwanie danych OpenAIRE...

Publikacje

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis

Autorzy: Mikhail Nikolaev, Olga Mitrofanova, Nicolas Broguiere, Sara Geraldo, Devanjali Dutta, Yoji Tabata, Bilge Elci, Nathalie Brandenberg, Irina Kolotuev, Nikolce Gjorevski, Hans Clevers, Matthias P. Lutolf
Opublikowane w: Nature, 585/7826, 2020, Page(s) 574-578, ISSN 0028-0836
Wydawca: Nature Publishing Group
DOI: 10.1038/s41586-020-2724-8

NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor

Autorzy: Laura Novellasdemunt, Anna Kucharska, Cara Jamieson, Maria Prange‐Barczynska, Anna Baulies, Pedro Antas, Jelte van der Vaart, Helmuth Gehart, Madelon M Maurice, Vivian SW Li
Opublikowane w: The EMBO Journal, 39/3, 2019, ISSN 0261-4189
Wydawca: Nature Publishing Group
DOI: 10.15252/embj.2019102771

Tissue-Engineering the Intestine: The Trials before the Trials

Autorzy: Hans Clevers, Ryan K. Conder, Vivian S.W. Li, Matthias P. Lutolf, Ludovic Vallier, Sarah Chan, Tracy C. Grikscheit, Kim B. Jensen, Paolo De Coppi
Opublikowane w: Cell Stem Cell, 24/6, 2019, Page(s) 855-859, ISSN 1934-5909
Wydawca: Cell Press
DOI: 10.1016/j.stem.2019.04.018

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Autorzy: Giovanni Giuseppe Giobbe, Claire Crowley, Camilla Luni, Sara Campinoti, Moustafa Khedr, Kai Kretzschmar, Martina Maria De Santis, Elisa Zambaiti, Federica Michielin, Laween Meran, Qianjiang Hu, Gijs van Son, Luca Urbani, Anna Manfredi, Monica Giomo, Simon Eaton, Davide Cacchiarelli, Vivian S. W. Li, Hans Clevers, Paola Bonfanti, Nicola Elvassore, Paolo De Coppi
Opublikowane w: Nature Communications, 10/1, 2019, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-019-13605-4

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Autorzy: Giovanni Canu, Emmanouil Athanasiadis, Rodrigo A. Grandy, Jose Garcia-Bernardo, Paulina M. Strzelecka, Ludovic Vallier, Daniel Ortmann, Ana Cvejic
Opublikowane w: Genome Biology, 21/1, 2020, ISSN 1474-760X
Wydawca: Springer Nature
DOI: 10.1186/s13059-020-02058-4

Genome-Wide Epigenetic and Transcriptomic Characterization of Human-Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Organoids

Autorzy: Judith Kraiczy, Alexander D.B. Ross, Jessica L. Forbester, Gordon Dougan, Ludovic Vallier, Matthias Zilbauer
Opublikowane w: Cellular and Molecular Gastroenterology and Hepatology, 7/2, 2019, Page(s) 285-288, ISSN 2352-345X
Wydawca: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2018.10.008

The Transcription Co-Repressors MTG8 and MTG16 Regulate Exit of Intestinal Stem Cells From Their Niche and Differentiation Into Enterocyte vs Secretory Lineages

Autorzy: Anna Baulies, Nikolaos Angelis, Valentina Foglizzo, E. Thomas Danielsen, Harshil Patel, Laura Novellasdemunt, Anna Kucharska, Joana Carvalho, Emma Nye, Paolo De Coppi, Vivian S.W. Li
Opublikowane w: Gastroenterology, 2020, ISSN 0016-5085
Wydawca: W. B. Saunders Co., Ltd.
DOI: 10.1053/j.gastro.2020.06.012

Tracing the origin of adult intestinal stem cells

Autorzy: Jordi Guiu, Edouard Hannezo, Shiro Yui, Samuel Demharter, Svetlana Ulyanchenko, Martti Maimets, Anne Jørgensen, Signe Perlman, Lene Lundvall, Linn Salto Mamsen, Agnete Larsen, Rasmus H. Olesen, Claus Yding Andersen, Lea Langhoff Thuesen, Kristine Juul Hare, Tune H. Pers, Konstantin Khodosevich, Benjamin D. Simons, Kim B. Jensen
Opublikowane w: Nature, 570/7759, 2019, Page(s) 107-111, ISSN 0028-0836
Wydawca: Nature Publishing Group
DOI: 10.1038/s41586-019-1212-5

The hope and the hype of organoid research

Autorzy: Meritxell Huch, Juergen A. Knoblich, Matthias P. Lutolf, Alfonso Martinez-Arias
Opublikowane w: Development, 144/6, 2017, Page(s) 938-941, ISSN 0950-1991
Wydawca: The Company of Biologists Ltd.
DOI: 10.1242/dev.150201

Multiscale microenvironmental perturbation of pluripotent stem cell fate and self-organization

Autorzy: Yoji Tabata, Matthias P. Lutolf
Opublikowane w: Scientific Reports, 7/1, 2017, ISSN 2045-2322
Wydawca: Nature Publishing Group
DOI: 10.1038/srep44711

Progress and potential in organoid research

Autorzy: Giuliana Rossi, Andrea Manfrin, Matthias P. Lutolf
Opublikowane w: Nature Reviews Genetics, 19/11, 2018, Page(s) 671-687, ISSN 1471-0056
Wydawca: Nature Publishing Group
DOI: 10.1038/s41576-018-0051-9

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration

Autorzy: Shiro Yui, Luca Azzolin, Martti Maimets, Marianne Terndrup Pedersen, Robert P. Fordham, Stine L. Hansen, Hjalte L. Larsen, Jordi Guiu, Mariana R.P. Alves, Carsten F. Rundsten, Jens V. Johansen, Yuan Li, Chris D. Madsen, Tetsuya Nakamura, Mamoru Watanabe, Ole H. Nielsen, Pawel J. Schweiger, Stefano Piccolo, Kim B. Jensen
Opublikowane w: Cell Stem Cell, 22/1, 2018, Page(s) 35-49.e7, ISSN 1934-5909
Wydawca: Cell Press
DOI: 10.1016/j.stem.2017.11.001

Mammalian body plan engineering: Lessons and challenges

Autorzy: Alfonso Martinez Arias, Matthias P. Lutolf
Opublikowane w: Current Opinion in Systems Biology, 11, 2018, Page(s) 50-56, ISSN 2452-3100
Wydawca: Elsevier
DOI: 10.1016/j.coisb.2018.07.007

Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon

Autorzy: Conor J. McCann, Julie E. Cooper, Dipa Natarajan, Benjamin Jevans, Laura E. Burnett, Alan J. Burns, Nikhil Thapar
Opublikowane w: Nature Communications, 8, 2017, Page(s) 15937, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/ncomms15937

Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling

Autorzy: Claudia Y. Janda, Luke T. Dang, Changjiang You, Junlei Chang, Wim de Lau, Zhendong A. Zhong, Kelley S. Yan, Owen Marecic, Dirk Siepe, Xingnan Li, James D. Moody, Bart O. Williams, Hans Clevers, Jacob Piehler, David Baker, Calvin J. Kuo, K. Christopher Garcia
Opublikowane w: Nature, 545/7653, 2017, Page(s) 234-237, ISSN 0028-0836
Wydawca: Nature Publishing Group
DOI: 10.1038/nature22306

Establishing Proximal and Distal Regional Identities in Murine and Human Tissue-Engineered Lung and Trachea

Autorzy: Andrew Trecartin, Soula Danopoulos, Ryan Spurrier, Hanaa Knaneh-Monem, Michael Hiatt, Barbara Driscoll, Christian Hochstim, Denise Al-Alam, Tracy C. Grikscheit
Opublikowane w: Tissue Engineering Part C: Methods, 22/11, 2016, Page(s) 1049-1057, ISSN 1937-3384
Wydawca: Mary Ann Liebert Inc.
DOI: 10.1089/ten.TEC.2016.0261

Functional Human and Murine Tissue-Engineered Liver Is Generated from Adult Stem/Progenitor Cells

Autorzy: Nirmala Mavila, Andrew Trecartin, Ryan Spurrier, Yi Xiao, Xiaogang Hou, David James, Xiaowei Fu, Brian Truong, Clara Wang, Gerald S. Lipshutz, Kasper S. Wang, Tracy C. Grikscheit
Opublikowane w: STEM CELLS Translational Medicine, 6/1, 2017, Page(s) 238-248, ISSN 2157-6564
Wydawca: AlphaMed Press
DOI: 10.5966/sctm.2016-0205

DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development

Autorzy: Judith Kraiczy, Komal M Nayak, Kate J Howell, Alexander Ross, Jessica Forbester, Camilla Salvestrini, Roxana Mustata, Sally Perkins, Amanda Andersson-Rolf, Esther Leenen, Anke Liebert, Ludovic Vallier, Philip C Rosenstiel, Oliver Stegle, Gordon Dougan, Robert Heuschkel, Bon-Kyoung Koo, Matthias Zilbauer
Opublikowane w: Gut, 68/1, 2018, Page(s) 49-61, ISSN 0017-5749
Wydawca: BMJ Publishing Group
DOI: 10.1136/gutjnl-2017-314817

Low‐Defect Thiol‐Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation

Autorzy: Saba Rezakhani, Nikolce Gjorevski, Matthias P. Lutolf
Opublikowane w: Advanced Functional Materials, 2020, Page(s) 2000761, ISSN 1616-301X
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202000761

SH3BP4 Regulates Intestinal Stem Cells and Tumorigenesis by Modulating β-Catenin Nuclear Localization

Autorzy: Pedro Antas, Laura Novellasdemunt, Anna Kucharska, Isobel Massie, Joana Carvalho, Dahmane Oukrif, Emma Nye, Marco Novelli, Vivian S.W. Li
Opublikowane w: Cell Reports, 26/9, 2019, Page(s) 2266-2273.e4, ISSN 2211-1247
Wydawca: Cell Press
DOI: 10.1016/j.celrep.2019.01.110

A Novel Human Pluripotent Stem Cell-Derived Neural Crest Model of Treacher Collins Syndrome Shows Defects in Cell Death and Migration

Autorzy: Felipe Serrano, William George Bernard, Alessandra Granata, Dharini Iyer, Ben Steventon, Matthew Kim, Ludovic Vallier, Laure Gambardella, Sanjay Sinha
Opublikowane w: Stem Cells and Development, 28/2, 2019, Page(s) 81-100, ISSN 1547-3287
Wydawca: Mary Ann Liebert Inc.
DOI: 10.1089/scd.2017.0234

Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure

Autorzy: Laween Meran, Isobel Massie, Sara Campinoti, Anne E. Weston, Riana Gaifulina, Lucinda Tullie, Peter Faull, Michael Orford, Anna Kucharska, Anna Baulies, Laura Novellasdemunt, Nikolaos Angelis, Elizabeth Hirst, Julia König, Alfonso Maria Tedeschi, Alessandro Filippo Pellegata, Susanna Eli, Ambrosius P. Snijders, Lucy Collinson, Nikhil Thapar, Geraint M. H. Thomas, Simon Eaton, Paola Bonfanti, Paol
Opublikowane w: Nature Medicine, 2019, ISSN 1078-8956
Wydawca: Nature Publishing Group
DOI: 10.1038/s41591-020-1024-z