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Multi-protein interaction kinetics by single molecule methods

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Publications

Single-Molecule Analysis beyond Dwell Times: Demonstration and Assessment in and out of Equilibrium

Author(s): Sonja Schmid, Markus Götz, Thorsten Hugel
Published in: Biophysical Journal, Issue 111/7, 2016, Page(s) 1375-1384, ISSN 0006-3495
DOI: 10.1016/j.bpj.2016.08.023

Multidomain structure and correlated dynamics determined by self-consistent FRET networks

Author(s): Björn Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias, Thorsten Hugel
Published in: Nature Methods, Issue 14/2, 2016, Page(s) 174-180, ISSN 1548-7091
DOI: 10.1038/nmeth.4081

Cooperative Nucleotide Binding in Hsp90 and Its Regulation by Aha1

Author(s): Philipp Wortmann, Markus Götz, Thorsten Hugel
Published in: Biophysical Journal, Issue 113/8, 2017, Page(s) 1711-1718, ISSN 0006-3495
DOI: 10.1016/j.bpj.2017.08.032

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Author(s): Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Published in: Nature Methods, Issue 15/9, 2018, Page(s) 669-676, ISSN 1548-7091
DOI: 10.1038/s41592-018-0085-0

Efficient use of single molecule time traces to resolve kinetic rates, models and uncertainties

Author(s): Sonja Schmid, Thorsten Hugel
Published in: The Journal of Chemical Physics, Issue 148/12, 2018, Page(s) 123312, ISSN 0021-9606
DOI: 10.1063/1.5006604

Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate

Author(s): Weixiang Ye, Markus Götz, Sirin Celiksoy, Laura Tüting, Christoph Ratzke, Janak Prasad, Julia Ricken, Seraphine V. Wegner, Rubén Ahijado-Guzmán, Thorsten Hugel, Carsten Sönnichsen
Published in: Nano Letters, Issue 18/10, 2018, Page(s) 6633-6637, ISSN 1530-6984
DOI: 10.1021/acs.nanolett.8b03342

Kinetics of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing and Fluorescence Stoichiometry

Author(s): Björn Hellenkamp, Johann Thurn, Martina Stadlmeier, Thorsten Hugel
Published in: The Journal of Physical Chemistry B, 2018, ISSN 1520-6106
DOI: 10.1021/acs.jpcb.8b07437