Multi-protein interaction kinetics by single molecule methods

Nanosecond structural dynamics of the chaperone Hsp90

Autores: Benedikt Sohmen, Christian Beck, Tilo Seydel, Ingo Hoffmann, Bianca Hermann, Mark Nüesch, Marco Grimaldo, Frank Schreiber, Steffen Wolf, Felix Roosen-Runge, Thorsten Hugel
Publicado en: arXiv, 2021
Editor: Cormell University

Inferring kinetic rate constants from single-molecule FRET trajectories – a blind benchmark of kinetic analysis tools

Autores: Markus Götz, Anders Barth, Søren S.-R. Bohr, Richard Börner, Jixin Chen, Thorben Cordes, Dorothy A. Erie, Christian Gebhardt, Mélodie C.A.S. Hadzic, George L. Hamilton, Nikos S. Hatzakis, Thorsten Hugel, Lydia Kisley, Don C. Lamb, Carlos de Lannoy, Chelsea Mahn, Dushani Dunukara, Dick de Ridder, Hugo Sanabria, Julia Schimpf, Claus A.M. Seidel, Roland K.O. Sigel, Magnus Berg Sletfjerding, Johan
Publicado en: bioRxiv, 2021
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.11.23.469671

The dynamics of the Hsp90 machine studied by single molecule FRET

Autores: Philipp Wortmann
Publicado en: 2017
Editor: TUM

Dissociation kinetics of protein complexes measured with a microfluidic fast dilution mixer on a confocal microscope using pulsed interleaved excitation

Autores: Johann Thurn
Publicado en: 2020
Editor: University of Freiburg

Single Protein Dynamics at Steady State Quantified from FRET Time Traces

Autores: Sonja Schmid
Publicado en: 2017
Editor: TUM

Multi-Color Single-Molecule FRET Experiments and Analysis

Autores: Markus Götz
Publicado en: 2017
Editor: TUM

Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations

Autores: Steffen Wolf, Benedikt Sohmen, Björn Hellenkamp, Johann Thurn, Gerhard Stock, Thorsten Hugel
Publicado en: Chemical Science, Edición 12/9, 2021, Página(s) 3350-3359, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/d0sc06134d

Autophosphorylation of the KaiC-like protein ArlH inhibits oligomerization and interaction with ArlI, the motor ATPase of the archaellum

Autores: J. Nuno de Sousa Machado, Leonie Vollmar, Julia Schimpf, Paushali Chaudhury, Rashmi Kumariya, Chris van der Does, Thorsten Hugel, Sonja-Verena Albers
Publicado en: Molecular Microbiology, Edición Volume116, Edición3, 2021, Página(s) 943, ISSN 1365-2958
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1111/mmi.14781

Controlling protein function by fine-tuning conformational flexibility

Autores: Sonja Schmid, Thorsten Hugel
Publicado en: eLife, Edición 9, 2020, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.57180

Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90

Autores: Katarzyna M. Tych, Markus Jahn, Florian Gegenfurtner, Vera K. Hechtl, Johannes Buchner, Thorsten Hugel, and Matthias Rief
Publicado en: J. Phys. Chem. B, Edición 122, 2018, Página(s) 11373, ISSN 1520-5207
Editor: American Chemical Society
DOI: 10.1021/acs.jpcb.8b07301

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Autores: Eitan Lerner , Anders Barth, Jelle Hendrix , Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N
Publicado en: eLife, 2021, Página(s) 10:e60416, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.60416

Single-Molecule Analysis beyond Dwell Times: Demonstration and Assessment in and out of Equilibrium

Autores: Sonja Schmid, Markus Götz, Thorsten Hugel
Publicado en: Biophysical Journal, Edición 111/7, 2016, Página(s) 1375-1384, ISSN 0006-3495
Editor: Biophysical Society
DOI: 10.1016/j.bpj.2016.08.023

Multidomain structure and correlated dynamics determined by self-consistent FRET networks

Autores: Björn Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias, Thorsten Hugel
Publicado en: Nature Methods, Edición 14/2, 2016, Página(s) 174-180, ISSN 1548-7091
Editor: Nature Publishing Group
DOI: 10.1038/nmeth.4081

Cooperative Nucleotide Binding in Hsp90 and Its Regulation by Aha1

Autores: Philipp Wortmann, Markus Götz, Thorsten Hugel
Publicado en: Biophysical Journal, Edición 113/8, 2017, Página(s) 1711-1718, ISSN 0006-3495
Editor: Biophysical Society
DOI: 10.1016/j.bpj.2017.08.032

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Autores: Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Publicado en: Nature Methods, Edición 15/9, 2018, Página(s) 669-676, ISSN 1548-7091
Editor: Nature Publishing Group
DOI: 10.1038/s41592-018-0085-0

Efficient use of single molecule time traces to resolve kinetic rates, models and uncertainties

Autores: Sonja Schmid, Thorsten Hugel
Publicado en: The Journal of Chemical Physics, Edición 148/12, 2018, Página(s) 123312, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/1.5006604

Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate

Autores: Weixiang Ye, Markus Götz, Sirin Celiksoy, Laura Tüting, Christoph Ratzke, Janak Prasad, Julia Ricken, Seraphine V. Wegner, Rubén Ahijado-Guzmán, Thorsten Hugel, Carsten Sönnichsen
Publicado en: Nano Letters, Edición 18/10, 2018, Página(s) 6633-6637, ISSN 1530-6984
Editor: American Chemical Society
DOI: 10.1021/acs.nanolett.8b03342

Kinetics of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing and Fluorescence Stoichiometry

Autores: Björn Hellenkamp, Johann Thurn, Martina Stadlmeier, Thorsten Hugel
Publicado en: The Journal of Physical Chemistry B, 2018, ISSN 1520-6106
Editor: American Chemical Society
DOI: 10.1021/acs.jpcb.8b07437

Effects of Inhibitors on Hsp90′s Conformational Dynamics, Cochaperone and Client Interactions

Autores: Sonja Schmid, Markus Götz, Thorsten Hugel
Publicado en: ChemPhysChem, Edición 19/14, 2018, Página(s) 1716-1721, ISSN 1439-4235
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cphc.201800342

Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors

Autores: Philipp Lohner, Mariia Zmyslia, Johann Thurn, Jasmin K. Pape, Rūta Gerasimaitė, Jan Keller‐Findeisen, Saskia Groeer, Benedikt Deuringer, Regine Süss, Andreas Walther, Stefan W. Hell, Gražvydas Lukinavičius, Thorsten Hugel, Claudia Jessen‐Trefzer
Publicado en: Angewandte Chemie International Edition, Edición 60/44, 2021, Página(s) 23835-23841, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202110327

A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously

Autores: Markus Götz, Philipp Wortmann, Sonja Schmid, Thorsten Hugel
Publicado en: Methods in Enzymology, Edición 581, 2016, Página(s) 487-516, ISSN 0076-6879
Editor: Academic Press
DOI: 10.1016/bs.mie.2016.08.024

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