Multi-protein interaction kinetics by single molecule methods

Nanosecond structural dynamics of the chaperone Hsp90

Autori: Benedikt Sohmen, Christian Beck, Tilo Seydel, Ingo Hoffmann, Bianca Hermann, Mark Nüesch, Marco Grimaldo, Frank Schreiber, Steffen Wolf, Felix Roosen-Runge, Thorsten Hugel
Pubblicato in: arXiv, 2021
Editore: Cormell University

Inferring kinetic rate constants from single-molecule FRET trajectories – a blind benchmark of kinetic analysis tools

Autori: Markus Götz, Anders Barth, Søren S.-R. Bohr, Richard Börner, Jixin Chen, Thorben Cordes, Dorothy A. Erie, Christian Gebhardt, Mélodie C.A.S. Hadzic, George L. Hamilton, Nikos S. Hatzakis, Thorsten Hugel, Lydia Kisley, Don C. Lamb, Carlos de Lannoy, Chelsea Mahn, Dushani Dunukara, Dick de Ridder, Hugo Sanabria, Julia Schimpf, Claus A.M. Seidel, Roland K.O. Sigel, Magnus Berg Sletfjerding, Johan
Pubblicato in: bioRxiv, 2021
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.11.23.469671

The dynamics of the Hsp90 machine studied by single molecule FRET

Autori: Philipp Wortmann
Pubblicato in: 2017
Editore: TUM

Dissociation kinetics of protein complexes measured with a microfluidic fast dilution mixer on a confocal microscope using pulsed interleaved excitation

Autori: Johann Thurn
Pubblicato in: 2020
Editore: University of Freiburg

Single Protein Dynamics at Steady State Quantified from FRET Time Traces

Autori: Sonja Schmid
Pubblicato in: 2017
Editore: TUM

Multi-Color Single-Molecule FRET Experiments and Analysis

Autori: Markus Götz
Pubblicato in: 2017
Editore: TUM

Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations

Autori: Steffen Wolf, Benedikt Sohmen, Björn Hellenkamp, Johann Thurn, Gerhard Stock, Thorsten Hugel
Pubblicato in: Chemical Science, Numero 12/9, 2021, Pagina/e 3350-3359, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d0sc06134d

Autophosphorylation of the KaiC-like protein ArlH inhibits oligomerization and interaction with ArlI, the motor ATPase of the archaellum

Autori: J. Nuno de Sousa Machado, Leonie Vollmar, Julia Schimpf, Paushali Chaudhury, Rashmi Kumariya, Chris van der Does, Thorsten Hugel, Sonja-Verena Albers
Pubblicato in: Molecular Microbiology, Numero Volume116, Numero3, 2021, Pagina/e 943, ISSN 1365-2958
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1111/mmi.14781

Controlling protein function by fine-tuning conformational flexibility

Autori: Sonja Schmid, Thorsten Hugel
Pubblicato in: eLife, Numero 9, 2020, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.57180

Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90

Autori: Katarzyna M. Tych, Markus Jahn, Florian Gegenfurtner, Vera K. Hechtl, Johannes Buchner, Thorsten Hugel, and Matthias Rief
Pubblicato in: J. Phys. Chem. B, Numero 122, 2018, Pagina/e 11373, ISSN 1520-5207
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.8b07301

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Autori: Eitan Lerner , Anders Barth, Jelle Hendrix , Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N
Pubblicato in: eLife, 2021, Pagina/e 10:e60416, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.60416

Single-Molecule Analysis beyond Dwell Times: Demonstration and Assessment in and out of Equilibrium

Autori: Sonja Schmid, Markus Götz, Thorsten Hugel
Pubblicato in: Biophysical Journal, Numero 111/7, 2016, Pagina/e 1375-1384, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2016.08.023

Multidomain structure and correlated dynamics determined by self-consistent FRET networks

Autori: Björn Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias, Thorsten Hugel
Pubblicato in: Nature Methods, Numero 14/2, 2016, Pagina/e 174-180, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/nmeth.4081

Cooperative Nucleotide Binding in Hsp90 and Its Regulation by Aha1

Autori: Philipp Wortmann, Markus Götz, Thorsten Hugel
Pubblicato in: Biophysical Journal, Numero 113/8, 2017, Pagina/e 1711-1718, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2017.08.032

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Autori: Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Pubblicato in: Nature Methods, Numero 15/9, 2018, Pagina/e 669-676, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-018-0085-0

Efficient use of single molecule time traces to resolve kinetic rates, models and uncertainties

Autori: Sonja Schmid, Thorsten Hugel
Pubblicato in: The Journal of Chemical Physics, Numero 148/12, 2018, Pagina/e 123312, ISSN 0021-9606
Editore: American Institute of Physics
DOI: 10.1063/1.5006604

Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate

Autori: Weixiang Ye, Markus Götz, Sirin Celiksoy, Laura Tüting, Christoph Ratzke, Janak Prasad, Julia Ricken, Seraphine V. Wegner, Rubén Ahijado-Guzmán, Thorsten Hugel, Carsten Sönnichsen
Pubblicato in: Nano Letters, Numero 18/10, 2018, Pagina/e 6633-6637, ISSN 1530-6984
Editore: American Chemical Society
DOI: 10.1021/acs.nanolett.8b03342

Kinetics of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing and Fluorescence Stoichiometry

Autori: Björn Hellenkamp, Johann Thurn, Martina Stadlmeier, Thorsten Hugel
Pubblicato in: The Journal of Physical Chemistry B, 2018, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.8b07437

Effects of Inhibitors on Hsp90′s Conformational Dynamics, Cochaperone and Client Interactions

Autori: Sonja Schmid, Markus Götz, Thorsten Hugel
Pubblicato in: ChemPhysChem, Numero 19/14, 2018, Pagina/e 1716-1721, ISSN 1439-4235
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cphc.201800342

Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors

Autori: Philipp Lohner, Mariia Zmyslia, Johann Thurn, Jasmin K. Pape, Rūta Gerasimaitė, Jan Keller‐Findeisen, Saskia Groeer, Benedikt Deuringer, Regine Süss, Andreas Walther, Stefan W. Hell, Gražvydas Lukinavičius, Thorsten Hugel, Claudia Jessen‐Trefzer
Pubblicato in: Angewandte Chemie International Edition, Numero 60/44, 2021, Pagina/e 23835-23841, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202110327

A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously

Autori: Markus Götz, Philipp Wortmann, Sonja Schmid, Thorsten Hugel
Pubblicato in: Methods in Enzymology, Numero 581, 2016, Pagina/e 487-516, ISSN 0076-6879
Editore: Academic Press
DOI: 10.1016/bs.mie.2016.08.024

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