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Transforming the future of agriculture through synthetic photorespiration

Deliverables

Synthetic pathways in E. coli

At least one synthetic pathway fully operational in E. coli

In vitro reconstructed pathways

At least one synthetic pathway fully operating using purified enzymes

Model of plant photosynthesis

A computational model of plant photosynthesis and metabolism that demonstrates the expected effect of the synthetic pathways on the rate of photosynthesis and carbon fixation.

Project website

Publication of the website of the project (public pages).

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Publications

A short history of RubisCO: the rise and fall (?) of Nature's predominant CO 2 fixing enzyme

Author(s): Tobias J Erb, Jan Zarzycki
Published in: Current Opinion in Biotechnology, Issue 49, 2018, Page(s) 100-107, ISSN 0958-1669
DOI: 10.1016/j.copbio.2017.07.017

Synthetic metabolism: metabolic engineering meets enzyme design

Author(s): Tobias J Erb, Patrik R Jones, Arren Bar-Even
Published in: Current Opinion in Chemical Biology, Issue 37, 2017, Page(s) 56-62, ISSN 1367-5931
DOI: 10.1016/j.cbpa.2016.12.023

Daring metabolic designs for enhanced plant carbon fixation

Author(s): Arren Bar-Even
Published in: Plant Science, Issue 273, 2018, Page(s) 71-83, ISSN 0168-9452
DOI: 10.1016/j.plantsci.2017.12.007

Enzyme engineering: reaching the maximal catalytic efficiency peak

Author(s): Moshe Goldsmith, Dan S Tawfik
Published in: Current Opinion in Structural Biology, Issue 47, 2017, Page(s) 140-150, ISSN 0959-440X
DOI: 10.1016/j.sbi.2017.09.002

Biochemical and synthetic biology approaches to improve photosynthetic CO2-fixation

Author(s): Tobias J Erb, Jan Zarzycki
Published in: Current Opinion in Chemical Biology, Issue 34, 2016, Page(s) 72-79, ISSN 1367-5931
DOI: 10.1016/j.cbpa.2016.06.026

Design and in vitro realization of carbon-conserving photorespiration

Author(s): Devin L. Trudeau, Christian Edlich-Muth, Jan Zarzycki, Marieke Scheffen, Moshe Goldsmith, Olga Khersonsky, Ziv Avizemer, Sarel J. Fleishman, Charles A. R. Cotton, Tobias J. Erb, Dan S. Tawfik, Arren Bar-Even
Published in: Proceedings of the National Academy of Sciences, Issue 115/49, 2018, Page(s) E11455-E11464, ISSN 0027-8424
DOI: 10.1073/pnas.1812605115

Synthetic biology approaches for improving photosynthesis

Author(s): Armin Kubis, Arren Bar-Even
Published in: Journal of Experimental Botany, Issue 70/5, 2019, Page(s) 1425-1433, ISSN 0022-0957
DOI: 10.1093/jxb/erz029