Risultati finali Documents, reports (34) Transcriptome associated with novel bioactive products in fish Report on the transcriptome analysis of larval stages and immune-related genes. High-throughput expression analysis will be performed on fish samples supplied with the bioactive compounds to evaluate regulatory pathways, using RNAseq technology Stimulation of growth by methanol Report on the effect of microalgae biomass production under mixotrophic conditions by addition of selected concentrations of methanol into the growth medium. Transcriptome associated with novel bioactive products in microalgae Report on the transcriptome analysis of selected microalgae strains grown under differential environmental and nutritional regimes associated with novel bioactive products, using RNAseq technology In vitro toxicity assessments of microalgae extracts or compounds Report on in vitro toxicity assessment of microalgae extracts or compounds using human dermal keratinocytes and normal human fibroblasts cell cultures Report on the quality control of the cosmeceutical products Report on the quality control of the cosmeceutical products at different storage conditions by using optical and physicochemical methods and microbiological testing. Optimal conditions of photonic quality Report on the optimization of microalgae biomass production under different light wavelengths, including white light, red light, green light and blue light. Metabolomic profile of microalgae under different culture regimes Report on the accumulation of a wide range of compounds spanning both primary and secondary metabolism under different growth conditions Report on the effect of microalgae extracts or compounds on the transcription of skin-related genes using human cell lines. Report on the the transcription effect of selected microalgae extracts or compounds on normal human keratinocytes and normal human fibroblasts in vitro using real-time RT-qPCR Catalomic profile of microalgae under different culture regimes Report on the activities of enzymes of cosmetic importance such as proteases, lipases, superoxide dismutase, catalase, peroxidase, under different growth conditions Final project evaluation and assessment report Final project evaluation and assessment report including external audits as necessary Report on outreach activities to the general public Report on the outreach activities aimed towards the general public including articles, videos etc. Proceedings of the training workshops Report on the proceeding and activities of the planned workshops which will be realised with in the project Establish the main biological effects of novel nutraceuticals on fish performance Report on the main biological effects of novel nutraceuticals on fish performance, including embryos from natural spawning of soles exposed to the biocompound to assess toxicity. Detailed report on the training and transfer of knowledge obtained through the secondments Report on the training and transfer of knowledge activities and skills obtained through the secondments plan for each secondee. Purification and characterization of hatching enzyme Report on the development of a proposed method for the purification and subsequent characterization of hatching enzyme, with a possible application in cosmetics Progress reports and project evaluation and assessment reports Detailed progress reports and progress evaluation and assessment reports including identification of possible risks during the project duration Glycomic profile of microalgae under different culture regimes Report on the yield and quality of polysaccharides production, including polysaccharide isolation, fractionation and structural characterization Establish protocols for nutraceuticals administration Report on the main biological effects of novel nutraceuticals on fish performance, including larval culture of soles under standard conditions and supplying microcapsules containing compounds until completion of metamorphosis. Identification and characterization of high-value added enzymes (proteases, lipases and antioxidant enzymes) with potential applications in cosmetology Report on the screening aiming at evaluating substrate specificity and activity of selected enzymes in algae extracts. The study will be focuses only on enzymes with interest in cosmetology Purification and structural analysis of selected algae polysaccharides Report on the purification and structural analysis of selected algae polysaccharides, including monosaccharide content determined by classical chromatography methods. Special attention will be paid on uronic and sulphate content which often carry the physico-chemical and biological properties of marine polysaccharides. Progress report Month 36 Reporting the clompletion of tasks, deliverables and milestons as set forth, including scientific repporting, financials, etc Progress report Month 12 Reporting the clompletion of tasks, deliverables and milestons as set forth, including scientific repporting, financials, etc... Comparative model of human and fish keratinocyte response to microalgal extracts Report on the comparative in vitro assessment and model development of human and fish keratinocyte response to microalgae extracts using selected in vitro enzyme assays and transcriptional effects. Define the ontogeny of immune barriers and effects of nutraceuticals Report on the ontogeny of immune barriers and effects of nutraceuticals, including survival and evaluation of growth, metamorphic success and malformations using skeletal staining techniques. Optimization of growth under various growth media Report on the optimization of microalgae biomass production in different growth media Optimal conditions of light intensity Report on the optimization of microalgae biomass production under different light intensities Minutes of all managements meetings held Minutes of all major meeting held for the management of the project Development of a fish epithelial model and assessment of toxicity and impact on the transcriptome and enzyme activities of microalgal extracts Report on the development of a fish epithelial model and assessment of toxicity and impact on the transcriptome and enzyme activities of microalgal extracts. Selected in vitro enzyme assays, transcriptional effects and in vitro toxicity/endocrine disruptor effects of microalgal extracts will included Transfer of the optimal growth conditions from lab scale to the large scale photobioreactors Report on the scale up and preculturing process would go through 500mL, 1L and 5L flasks to the 50L PBR reactors placed inside the artificial light room and finally to the outdoor PBRs placed inside a pilot plant greenhouse. Proceedings of the Summer schools Report on the proceeding and activities of summer schools which will be realised with in the project Intellectual property and networking activities report Report on the management of Intellectual property and networking activities management between the members of the consortium Mapping bioactive properties using in vitro enzyme bioassays Report on the screening and examination of bioactivity of algae extracts by evaluating the inhibition potency of extracts towards enzyme targets of importance for the cosmetic industry Steady-state kinetic characterization of enzymes and thermodynamics analysis of the enzyme stability Report on the kinetic and stability characterization of the new isolated enzymes. Steady-state kinetic analysis will be used to characterize the kinetic parameters of the enzymes. A downstream processing protocol for enzymes purification Report on the development of an application based downstream processing protocol for enzymes purification with an emphasis in the development of a single-step, low cost and environmental friendly method for enzyme purification Other (3) Publications in scientific journals/conferences Scientific publications in international peer-review scientific journals and conferences attended by members of the consortium Biannual e-newsletters A bi-annual e-newsletter will inform end users and stakeholders on scientific progress, job opportunities and upcoming events regarding the scope of this project. Analytical Handbook An analytical handbook containing main protocols and procedures developed by the partners with in the duration of the project Websites, patent fillings, videos etc. (2) ALGAE4A-B Project Website A project website containing all the information regarding the scope and activities of the consortium during the implementation of the project Training and e-learning web-site A web-site containing Training and e-learning material produced by the project Pubblicazioni Peer reviewed articles (7) Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis Autori: Joana Firmino, Carlos Carballo, Paula Armesto, Marco A. Campinho, Deborah M. Power, Manuel Manchado Pubblicato in: BMC Developmental Biology, Issue 17/1, 2017, ISSN 1471-213X Editore: BioMed Central DOI: 10.1186/s12861-017-0154-0 Antioxidant capacity and immunomodulatory effects of a chrysolaminarin-enriched extract in Senegalese sole Autori: Carlos Carballo, Evangelia G. Chronopoulou, Sofia Letsiou, Claudia Maya, Nikolaos E. Labrou, Carlos Infante, Deborah M. Power, Manuel Manchado Pubblicato in: Fish & Shellfish Immunology, Issue 82, 2018, Page(s) 1-8, ISSN 1050-4648 Editore: Academic Press DOI: 10.1016/j.fsi.2018.07.052 Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach Autori: Patricia I. S. Pinto, Cláudia C. Guerreiro, Rita A. Costa, Juan F. Martinez-Blanch, Carlos Carballo, Francisco M. Codoñer, Manuel Manchado, Deborah M. Power Pubblicato in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322 Editore: Nature Publishing Group DOI: 10.1038/s41598-019-49501-6 Transcriptomic and metabolomic adaptation of Nannochloropsis gaditana grown under different light regimes Autori: Maria Patelou, Carlos Infante, Flavien Dardelle, Dörte Randewig, Evangelia D. Kouri, Michael K. Udvardi, Eleni Tsiplakou, Lalia Mantecón, Emmanouil Flemetakis Pubblicato in: Algal Research, Issue 45, 2020, Page(s) 101735, ISSN 2211-9264 Editore: Elsevier BV DOI: 10.1016/j.algal.2019.101735 Cartilage acidic protein 1 promotes increased cell viability, cell proliferation and energy metabolism in primary human dermal fibroblasts Autori: Sophia Letsiou, Rute Felix, João C. Cardoso, Liliana Anjos, Ana L. Mestre, Henirique L. Gomes, Deborah M. Power Pubblicato in: Biochimie, 2020, ISSN 0300-9084 Editore: Elsevier BV DOI: 10.1016/j.biochi.2020.02.008 Yeast β-glucans and microalgal extracts modulate the immune response and gut microbiome in Senegalese sole (Solea senegalensis) Autori: Carlos Carballo, Patricia I.S. Pinto, Ana Patricia Mateus, Concha Berbel, Cláudia C. Guerreiro, Juan F. Martinez-Blanch, Francisco M. Codoñer, Lalia Mantecon, Deborah M. Power, Manuel Manchado Pubblicato in: Fish & Shellfish Immunology, Issue 92, 2019, Page(s) 31-39, ISSN 1050-4648 Editore: Academic Press DOI: 10.1016/j.fsi.2019.05.044 Genomic and phylogenetic analysis of choriolysins, and biological activity of hatching liquid in the flatfish Senegalese sole Autori: Carlos Carballo, Evangelia G. Chronopoulou, Sophia Letsiou, Eleni Spanidi, Konstantinos Gardikis, Nikolaos E. Labrou, Manuel Manchado Pubblicato in: PLOS ONE, Issue 14/12, 2019, Page(s) e0225666, ISSN 1932-6203 Editore: Public Library of Science DOI: 10.1371/journal.pone.0225666 Conference proceedings (9) Chrysolaminarin encapsulation in cyclodextrins and hydrolyzed rice flour. In vitro and in vivo efficacy studies on human dermal fibroblasts and fish larvae Autori: Konstantinos Gardikis, Manuel Manchado, Israel Guerrero-Cózar, Claudia Maya, Carlos Infante, Sophia Letsiou, Vasiliki-Ioanna Boka, William Helbert Pubblicato in: BIT’s 8th Annual International Symposium of Drug Delivery Systems (SDDS-2018), 2018 Editore: Not known Marine products as effective raw materials for cosmetics – a research and innovation approach Autori: Konstantinos Gardikis, Sophia Letsiou Pubblicato in: 30th International Symposium on the Chemistry of Natural Products, 2018 Editore: Not known In vitro effects of cyanobacteria exopolysaccharides on primary human dermal cells Autori: Ioanna Anastasiou, Katerina I. Kalliampakou, Sophia Letsiou, Carlos Infante , William Helbert, Kostantinos Gardikis, Lalia Mantecon and Emmanouil Flemetakis. Pubblicato in: 10th European Conferenceon Marine Natural Products proceedings, 2017 Editore: 10th European Conferenceon Marine Natural Products proceedings Electric Cell-substrate Impedance Sensing (ECIS) as a method to test marine bioactive compounds in wound healing Autori: Félix RC, Letsiou S, Anjos L, Gomes HL, Power DM Pubblicato in: 4th Conference on Impedance-Based Cell Assays (IBCA2018), 2018 Editore: IBCA Microalgal polysaccharide extracts modulate the immune response and microbiome in the gut of Senegalese sole (Solea Senegalensis) Autori: Patrícia Pinto, C Carballo, AP Mateus, J Martinez-Blanch, F Codoner, L Mantecon, M Manchado, DM Power Pubblicato in: 31th New European Soc. for Comparative Physiology and Biochemistry (ESCPB 2018), 2018 Editore: Not known In vitro antioxidant and wound healing effects of cyanobacteria exopolysaccharides on primary human fibroblasts Autori: Ioanna Anastasiou, Sophia Letsiou, Katerina I. Kalliampakou, Carlos Infante , William Helbert, Kostantinos Gardikis, Lalia Mantecón and Emmanouil Flemetakis Pubblicato in: Proceedings of the 68th Helenic Biochemistry and Molecular Biology Society, 2017 Editore: 68th Helenic Biochemistry and Molecular Biology Society In vitro effects of Cartilage acidic protein 1 on primary human dermal fibroblasts Autori: Anjos L, Cardoso JCR, Gardikis K, Power DM and Letsiou S Pubblicato in: 10th European Conferenceon Marine Natural Products proceedings, 2017 Editore: 10th European Conferenceon Marine Natural Products proceedings Genomic and epigenetic advances in sole aquaculture Autori: Manuel Manchado Pubblicato in: 2018 Editore: SIBIC2018, the VII Congress of the Iberian Society for Ichthyology Omics signatures of pseudo-albino in Senegalese sole Autori: Pinto PIS, Guerreiro C, Costa RA, Martinez-Blanch JF, Carballo C, Codoñer F, Manchado M, Power DM Pubblicato in: VII Congress of the Iberian Soc. for Ichthyology, 2018 Editore: Proceedings of the VII Congress of the Iberian Soc. for Ichthyology Other (4) LA REPROGRAMACION EPIGENÉTICA CON EXTRACTOS DE MICROALGAS MODIFICA LA TOLERANCIA A LA TEMPERATURA EN LARVAS DE LENGUADO Autori: Ana Manuela Crespo1, Carlos Carballo1, Lalia Mantecón2y Manuel Manchado1 Pubblicato in: 2018 Editore: Not known CHARACTERIZATION OF HATCHING ENZYMES IN SOLE (S. SENEGALENSIS) BY USING GENOMIC APPROACHES TO BE USED AS A NEW BIOTECHNOLOGICAL PRODUCT Autori: Carballo C.1*, Chronopoulou E.2, Crespo A.1, Gardikis K.3, Labrou N.2, Manchado M. Pubblicato in: International Flatfish Symposium 2017, 2017 Editore: International Flatfish Symposium 2017 Estudio de las propiedades antioxidantes e inmunomoduladoras de la crisolaminarina Autori: Carlos Carballo, Evangelia G. Chronopoulou; Sofia Letsiou; Claudia Maya; Nikolaos E. Labrou; Carlos Infante; Deborah M. Power; Manuel Manchado Pubblicato in: IX Jornadas de Acuicultura en el Litoral Suratlántico - La Acuicultura Progresa, 2018 Editore: IX Jornadas de Acuicultura en el Litoral Suratlántico The use of fish mineralized tissues to assess estrogenic endocrine disruption and other ongoing transcriptome and microbiome studies at CCMAR (including Algae4ab) Autori: P. Pinto (CCMAR) Pubblicato in: Invited talk at research centre UMR I-02 SEBIO, University le Havre-Normandie (France), 2019 Editore: NA Thesis and dissertations (1) Genetic estimates and immune response associated with the Lymphocystis Disease Virus (LCDV) in marine fish Autori: C. Carballo Pubblicato in: 2020 Editore: University of Malaga È in corso la ricerca di dati su OpenAIRE... Si è verificato un errore durante la ricerca dei dati su OpenAIRE Nessun risultato disponibile
