Artificial endosymbiosis

Project Information

ARTISYM

Grant agreement ID: 694120

DOI 10.3030/694120

Project closed

EC signature date 24 October 2016

Start date 1 January 2017

End date 31 December 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 500 000,00

EU contribution € 2 500 000,00

Coordinated by

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

Publications

Peer reviewed articles (36)

Dual Site-Selective Presentation of Functional Handles on Protein-Engineered Cowpea Chlorotic Mottle Virus-Like Particles

Author(s): Daan F. M. Vervoort, Robin Heiringhoff, Suzanne B. P. E. Timmermans, Marleen H. M. E. van Stevendaal, Jan C. M. van Hest
Published in: Bioconjugate Chemistry, Issue 32/5, 2021, Page(s) 958-963, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00108

Therapeutic Stomatocytes with Aggregation Induced Emission for Intracellular Delivery

Author(s): Jingxin Shao, Shoupeng Cao, Hanglong Wu, Loai KEA Abdelmohsen, Jan C.M. van Hest
Published in: PHARMACEUTICS, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13111833

Amphiphilic AIEgen-polymer aggregates: Design, self-assembly and biomedical applications

Author(s): Shoupeng Cao,Jingxin Shao,Loai K. E. A. Abdelmohsen,Jan C. M. van Hest
Published in: Aggregate, 2021, ISSN 2692-4560
Publisher: John Wiley and Sons
DOI: 10.1002/agt2.128

Photoactivated Polymersome Nanomotors: Traversing Biological Barriers

Author(s): Jingxin Shao, Shoupeng Cao, David S. Williams, Loai K. E. A. Abdelmohsen, Jan C. M. Hest
Published in: Angewandte Chemie International Edition, Issue 59/39, 2020, Page(s) 16918-16925, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202003748

Biodegradable Polymersomes with Structure Inherent Fluorescence and Targeting Capacity for Enhanced Photo-Dynamic Therapy

Author(s): Shoupeng Cao,Yifeng Xia,Jingxin Shao,Beibei Guo,Yangyang Dong, Imke A. B. Pijpers, Zhiyuan Zhong,Fenghua Meng, Loai K. E. A. Abdelmohsen,David S. Williams, Jan C. M. van Hest
Published in: Angewandte Chemie Int Ed, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202105103

Pathway dependent shape-transformation of azide-decorated polymersomes

Author(s): Hailong Che, Lafayette N. J. de Windt, Jianzhi Zhu, Imke A. B. Pijpers, Alexander F. Mason, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Published in: Chemical Communications, Issue 56/14, 2020, Page(s) 2127-2130, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9cc08944f

Programmed spatial organization of biomacromolecules into discrete, coacervate-based protocells

Author(s): Wiggert J. Altenburg, N. Amy Yewdall, Daan F. M. Vervoort, Marleen H. M. E. van Stevendaal, Alexander F. Mason, Jan C. M. van Hest
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-20124-0

Cucurbit-Like Polymersomes with Aggregation-Induced Emission Properties Show Enzyme-Mediated Motility

Author(s): Shoupeng Cao, Hanglong Wu, Imke A. B. Pijpers, Jingxin Shao, Loai K. E. A. Abdelmohsen, David S. Williams, Jan C. M. van Hest
Published in: ACS Nano, 2021, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.1c07343

Engineering transient dynamics of artificial cells by stochastic distribution of enzymes

Author(s): Shidong Song, Alexander F. Mason, Richard AJ Post, Marco De Corato, Rafael Mestre, N Amy Yewdall, Shoupeng Cao, Remco W van der Hofstad, Samuel Sanchez, Loai KEA Abdelmohsen, Jan C.M. van Hest
Published in: Nature Communications, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-27229-0

Artificial Organelles: Towards Adding or Restoring Intracellular Activity

Author(s): Roy A. J. F. Oerlemans, Suzanne B. P. E. Timmermans, Jan C. M. Hest
Published in: ChemBioChem, Issue 22/12, 2021, Page(s) 2051-2078, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000850

Engineering of Biocompatible Coacervate-Based Synthetic Cells

Author(s): Marleen H. M. E. van Stevendaal, Laurynas Vasiukas, N. Amy Yewdall, Alexander F. Mason, Jan C. M. van Hest
Published in: ACS Applied Materials & Interfaces, Issue 13/7, 2021, Page(s) 7879-7889, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.0c19052

Biomimicry of Cellular Motility and Communication Based on Synthetic Soft-Architectures

Author(s): Lei Wang, Shidong Song,Jan C.M. van Hest, Loai KEA Abdelmohsen, Xin Huang, Samuel Sanchez
Published in: Small, 2021, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201907680

Intercellular communication between artificial cells by allosteric amplification of a molecular signal

Author(s): Bastiaan C. Buddingh’, Janneke Elzinga, Jan C. M. van Hest
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-15482-8

Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform

Author(s): Alexander F. Mason,Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal, Jan C.M. van Hest
Published in: Methods in enzymology, 2021, ISSN 0076-6879
Publisher: Academic Press
DOI: 10.1016/bs.mie.2020.06.008

Morphology Under Control: Engineering Biodegradable Stomatocytes

Author(s): Imke A. B. Pijpers, Loai K. E. A. Abdelmohsen, David S. Williams, Jan C. M. van Hest
Published in: ACS Macro Letters, Issue 6/11, 2017, Page(s) 1217-1222, ISSN 2161-1653
Publisher: American Chemical Society
DOI: 10.1021/acsmacrolett.7b00723

Feedback-Induced Temporal Control of “Breathing” Polymersomes To Create Self-Adaptive Nanoreactors

Author(s): Hailong Che, Shoupeng Cao, Jan C. M. van Hest
Published in: Journal of the American Chemical Society, Issue 140/16, 2018, Page(s) 5356-5359, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b02387

Hierarchical Self-Assembly of a Copolymer-Stabilized Coacervate Protocell

Author(s): Alexander F. Mason, Bastiaan C. Buddingh’, David S. Williams, Jan C. M. van Hest
Published in: Journal of the American Chemical Society, Issue 139/48, 2017, Page(s) 17309-17312, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b10846

Biodegradable Synthetic Organelles Demonstrate ROS Shielding in Human-Complex-I-Deficient Fibroblasts

Author(s): Lisanne M. P. E. van Oppen, Loai K. E. A. Abdelmohsen, Sjenet E. van Emst-de Vries, Pascal L. W. Welzen, Daniela A. Wilson, Jan A. M. Smeitink, Werner J. H. Koopman, Roland Brock, Peter H. G. M. Willems, David S. Williams, Jan C.M. van Hest
Published in: ACS Central Science, Issue 4/7, 2018, Page(s) 917-928, ISSN 2374-7943
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.8b00336

Self-Assembly and Stabilization of Hybrid Cowpea Chlorotic Mottle Virus Particles under Nearly Physiological Conditions

Author(s): Suzanne B. P. E. Timmermans, Daan F. M. Vervoort, Lise Schoonen, Roeland J. M. Nolte, Jan C. M. van Hest
Published in: Chemistry - An Asian Journal, Issue 13/22, 2018, Page(s) 3518-3525, ISSN 1861-4728
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/asia.201800842

Erythrocyte Membrane Modified Janus Polymeric Motors for Thrombus Therapy

Author(s): Jingxin Shao, Mona Abdelghani, Guizhi Shen, Shoupeng Cao, David S. Williams, Jan C. M. van Hest
Published in: ACS Nano, Issue 12/5, 2018, Page(s) 4877-4885, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.8b01772

Physicochemical Characterization of Polymer‐stabilized Coacervate Protocells

Author(s): N. Amy Yewdall, Bastiaan C. Buddingh’, Wiggert J. Altenburg, Suzanne B. P. E. Timmermans, Daan F. M. Vervoort, Loai K.E.A. Abdelmohsen, Alexander F. Mason, Jan van Hest
Published in: ChemBioChem, 2019, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201900195

The hallmarks of living systems: towards creating artificial cells

Author(s): N. Amy Yewdall, Alexander F. Mason, Jan C. M. van Hest
Published in: Interface Focus, Issue 8/5, 2018, Page(s) 20180023, ISSN 2042-8898
Publisher: Royal Society Publishing
DOI: 10.1098/rsfs.2018.0023

Development of Morphologically Discrete PEG–PDLLA Nanotubes for Precision Nanomedicine

Author(s): Annelies C. Wauters, Imke A. B. Pijpers, Alexander F. Mason, David S. Williams, Jurjen Tel, Loai K. E. A. Abdelmohsen, Jan C.M. van Hest
Published in: Biomacromolecules, Issue 20/1, 2018, Page(s) 177-183, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.8b01245

Biomorphic Engineering of Multifunctional Polylactide Stomatocytes toward Therapeutic Nano-Red Blood Cells

Author(s): Jingxin Shao, Imke A. B. Pijpers, Shoupeng Cao, David S. Williams, Xuehai Yan, Junbai Li, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Published in: Advanced Science, Issue 6/5, 2019, Page(s) 1801678, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.201801678

pH-Induced Transformation of Biodegradable Multilamellar Nanovectors for Enhanced Tumor Penetration

Author(s): Shoupeng Cao, Loai K. E. A. Abdelmohsen, Jingxin Shao, Joep van den Dikkenberg, Enrico Mastrobattista, David S. Williams, Jan C. M. van Hest
Published in: ACS Macro Letters, Issue 7/11, 2018, Page(s) 1394-1399, ISSN 2161-1653
Publisher: American Chemical Society
DOI: 10.1021/acsmacrolett.8b00807

Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization

Author(s): Alexander F. Mason, N. Amy Yewdall, Pascal L. W. Welzen, Jingxin Shao, Marleen van Stevendaal, Jan C. M. van Hest, David S. Williams, Loai K. E. A. Abdelmohsen
Published in: ACS Central Science, Issue 5/8, 2019, Page(s) 1360-1365, ISSN 2374-7943
Publisher: ACS
DOI: 10.1021/acscentsci.9b00345

Adaptive Polymeric Assemblies for Applications in Biomimicry and Nanomedicine

Author(s): Yigit Altay, Shoupeng Cao, Hailong Che, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Published in: Biomacromolecules, Issue 20/11, 2019, Page(s) 4053-4064, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.9b01341

Adaptive Polymersome Nanoreactors

Author(s): Hailong Che, Jan C. M. van Hest
Published in: ChemNanoMat, Issue 5/9, 2019, Page(s) 1092-1109, ISSN 2199-692X
Publisher: Wiley
DOI: 10.1002/cnma.201900245

Self-Regulated and Temporal Control of a “Breathing” Microgel Mediated by Enzymatic Reaction

Author(s): Hailong Che, Bastiaan C. Buddingh', Jan C. M. van Hest
Published in: Angewandte Chemie International Edition, Issue 56/41, 2017, Page(s) 12581-12585, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201706716

Molecular Programming of Biodegradable Nanoworms via Ionically Induced Morphology Switch toward Asymmetric Therapeutic Carriers

Author(s): Shoupeng Cao, Jingxin Shao, Yifeng Xia, Hailong Che, Zhiyuan Zhong, Fenghua Meng, Jan C. M. van Hest, Loai K. E. A. Abdelmohsen, David S. Williams
Published in: Small, Issue 15/38, 2019, Page(s) 1901849, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201901849

Photoactivated nanomotors via aggregation induced emission for enhanced phototherapy

Author(s): Shoupeng Cao, Jingxin Shao, Hanglong Wu, Shidong Song, Maria Teresa De Martino, Imke A. B. Pijpers, Heiner Friedrich, Loai K. E. A. Abdelmohsen, David S. Williams, Jan C. M. van Hest
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-22279-w

ATP‐Mediated Transient Behavior of Stomatocyte Nanosystems

Author(s): Hailong Che, Jianzhi Zhu, Shidong Song, Alexander F. Mason, Shoupeng Cao, Imke A. B. Pijpers, Loai K. E. A. Abdelmohsen, Jan C. M. Hest
Published in: Angewandte Chemie, Issue 131/37, 2019, Page(s) 13247-13252, ISSN 0044-8249
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201906331

Compartmentalized cross-linked enzymatic nano -aggregates ( c -CLE n A) for efficient in-flow biocatalysis

Author(s): M. Teresa De Martino, Fabio Tonin, N. Amy Yewdall, Mona Abdelghani, David S. Williams, Ulf Hanefeld, Floris P. J. T. Rutjes, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Published in: Chemical Science, Issue 11/10, 2020, Page(s) 2765-2769, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sc05420k

Dynamic spatial and structural organization in artificial cells regulates signal processing by protein scaffolding

Author(s): Bastiaan C. Buddingh', Antoni Llopis-Lorente, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest
Published in: Chemical Science, Issue 11/47, 2020, Page(s) 12829-12834, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc03933k

Hybrid Biodegradable Nanomotors through Compartmentalized Synthesis

Author(s): Imke A. B. Pijpers, Shoupeng Cao, Antoni Llopis-Lorente, Jianzhi Zhu, Shidong Song, Rick R. M. Joosten, Fenghua Meng, Heiner Friedrich, David S. Williams, Samuel Sánchez, Jan C. M. van Hest, Loai K. E. A. Abdelmohsen
Published in: Nano Letters, Issue 20/6, 2020, Page(s) 4472-4480, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.0c01268

Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs

Author(s): Eva Magdalena Estirado, Alexander F. Mason, Miguel Ángel Alemán García, Jan C. M. van Hest, Luc Brunsveld
Published in: Journal of the American Chemical Society, Issue 142/20, 2020, Page(s) 9106-9111, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c01732