Seed mucilage envelope as a new structural model in cell wall research

Información del proyecto

MuCellWall

Identificador del acuerdo de subvención: 702293

DOI

10.3030/702293

Proyecto cerrado

Fecha de la firma de la CE 12 Abril 2016

Fecha de inicio 1 Septiembre 2016

Fecha de finalización 31 Agosto 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 171 460,80

Aportación de la UE

€ 171 460,80

Coordinado por

CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL
Germany

Objetivo

The plant cell wall is dynamic, complexes structure composed of polysaccharides divided into fibrillar (cellulosic) fraction and extracellular matrix (hemicelluloses and pectins). All the components are arranged in a network structure characteristic of the cell wall. The proposed, general spatial models of the cell wall based mostly on various biochemical and/or microstructural analysis, which have usually been invasive by treatment with enzymes or chemicals, which can destroy the cell wall organization and allow to visualize a selected components. Despite of extensive research our knowledge about the cell wall is still incomplete and restricted mostly to the biochemical or genetic studies. The main aim of this project will be the determination of the cell wall components i.e. polysaccharides, glycoproteins, and the determination their spatial distribution in a special type of cell wall of the mucilage secreting cells (MSCs). The obtained results will allow creation a direct spatial cell wall model. The used material e.g. seeds of Arabidopsis thaliana mutants and wild type, Artemisia leucodens or Lepidium sativum will be an excellent and so far not examined study model due to characteristic loose spatial structure of the MSC cell wall. The use of modern techniques (in a quite new combinations) e.g. antibodies with attached gold particles and precise visualization in divers microscopes (e.g. SEM, LCSM) available at the host laboratory (Kiel University) will allow to overcome invasive procedures and let to detect the exactly location of the components. The realistic, representative cell wall spatial model will be created allowing for better understanding the different processes taking place within the cell wall. The project will be implemented at an interdisciplinary level opening a new possibilities for various cell wall studies. The project will give a new insight into the cell wall structural studies and lead to significant advances in this field.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias físicas › óptica › microscopía
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › carbohidratos
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas › enzima

Palabras clave

• Arabidopsis thaliana
• cell wall
• mucilage
• cellulose
• pectins
• hemicelluloses
• seeds
• cell wall model

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL

• Aportación neta de la UEn: € 171 460,80
• Dirección: OLSHAUSENSTRASSE 40 ; 24118 Kiel ; Alemania
• Región: Schleswig-Holstein › Schleswig-Holstein › Kiel, Kreisfreie Stadt
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 171 460,80