Concerning the history of intersexuality, one of the methodological problems that arose is the search of primary sources. The same process of infancy medicalization and the pathologization of intersexuality in Colombia has been evidenced in the absence of references to intersexual individual during the beginning of the 20th century. Nevertheless, in general journals of medicine, and in the ones of specialties like pediatrics or obstetrics, is evident an increasing interest for subjects related to children sexuality, childcare (puericultura), prenatal “sex predetermination” and “sex diagnostic”, circumcision, amenorrhea, homosexuality, sexual precocity as well as feminine or masculine genital “pathologies” such as cryptorchidism and hypospadias, among others. These matters allow to evidence determinant categories to construct the normal parameters used by Colombian physicians when they needed to delimitate the meaning of being a woman or a man since the birth. Having into account some merges of categories as degeneration with sexual precocity and normal or abnormal behavior of children, hormonal treatments to the children behavior, or endocrinology and homosexuality, 136 articles of the 355 recovered between 1900 and 1980 in Colombian medical journals. In addition, among the 355 publications recovered, 25 were specifically about intersexuality. They could be classified by the medical category associated to each case, such as: Pseudohermaphroditism, Genitals malformations, Intersexuality, Pituitary Failure, Hermaphroditism, Adrenogenital syndrome, Turner, Congenital adrenal hyperplasia, True hermaphroditism, Gynecomastia and Testicular feminization. Finally, the results achieve were communicated in sixteen national and international conferences, five articles were redacted and the courses imparted by the researcher has been feed by the findings.