Development of high-speed microscopes to study wide-scale neural activity

Información del proyecto

WideBrainImaging

Identificador del acuerdo de subvención: 704698

DOI

10.3030/704698

Proyecto finalizado el 31 Marzo 2016

Fecha de la firma de la CE 18 Febrero 2016

Fecha de inicio 1 Marzo 2016

Fecha de finalización 28 Febrero 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 178 156,80

Aportación de la UE

€ 178 156,80

Coordinado por

FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH
Austria

Objetivo

The dynamic of neural computation is often studied in individual cells using inserted electrodes, or using low-resolution methods such as EEG. Functional fluorescent imaging has recently emerged as a powerful complementary tool that allows single-cell resolution of relatively large networks, opening a new regime to neuroscience. However, complex brains are generally opaque and can only be studied with scanning two-photon microscopes; with the achievable depth limited to ~0.6mm and the volume limited by the relatively slow scan.
This project will develop ultrafast scanning multiphoton microscopes to image neural activity at cellular resolution over large volumes, and at greater depth. Using these we will study patterns of activity in the hippocampus, and particularly attempt to observe the pathways involved in memory retention. To increase speed we will use temporal focusing to controllably sculpt the excitation volume and enlarge the focal spot. This reduces the number of measured pixels and allows faster scanning (or larger volume), at the cost of resolution. This will allow 25x faster imaging in a resonant scanning two-photon microscope; allowing observation of many thousands of cells at once, which could reveal the wide-scale characteristic activity.
We will build a second microscope that uses three-photon excitation with temporal focusing. Three-photon imaging relies on longer wavelengths that penetrate deeper into tissue, and also suppresses background fluorescence which could otherwise limit depth. Consequently, this microscope will allow high-speed imaging at depth exceeding 1mm. This allows study of information transfer across multiple layers; or provides access to the hippocampus through the intact cortex. These studies could provide crucial insights to neuroscience that are currently accessible only for a few neurons.
Following completion of this project these microscopes could have an enduring impact as they continue to be used to study neural dynamics.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › neurobiología
• ciencias naturales › ciencias químicas › química inorgánica › metales alcalinotérreos
• ciencias naturales › ciencias físicas › óptica › microscopía
• ciencias naturales › informática y ciencias de la información › inteligencia artificial › aprendizaje automático
• ciencias naturales › ciencias físicas › óptica › física del láser

Palabras clave

• Functional fluorescent imaging
• scanning laser microscope
• multiphoton fluorescence

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH

• Aportación neta de la UEn: € 178 156,80
• Dirección: CAMPUS-VIENNA-BIOCENTER 1 ; 1030 Wien ; Austria
• Región: Ostösterreich › Wien › Wien
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: € 178 156,80