This project included both training and scientific objectives. During year 1, we generated a novel BAT-specific knockout mouse model to study hormonal regulation of adipose thermogenesis. While this was being generated, we performed circadian studies in cells and control animals to determine circadian gene expression patterns under basal conditions, and also performed cold-exposure studies to determine how thermal stress regulates gene transcription in mice. During this period, we also used an in vitro cell culture system to investigate cell signaling pathways activated by adrenergic signaling and generate a model of cold-activation of cell signaling pathways regulated by the processes of thermal stress. During this time, there was a strong focus on professional development, including attending international conferences and trainings, as well as several courses and workshops in Denmark focused on scientific writing, grant writing, career choices and didactic skills. PRofessional leadership development was nurtured through service on the board of the European Young Endocrine Scientists organization that included chairing scientific sessions at meetings.
During the second year, we utilized the novel mice generated during the first year to investigate the thermogenic and circadian regulation of adipose thermogenesis in knockout animal models using state-of-the-art technologies and techniques such as telemetric temperature and activity recordings. Systemic metabolic effects were determined using indirect calorimetry and insulin and glucose tolerance tests. We also determined the potential translational potential of our discoveries using human immortal adipose cultures and primary human adipocytes. In conjunction with this, we analyzed genetic datasets to determine the impact of mutations in some of our key interest genes on human metabolism. Professional development activities continued through the second year, including attending conferences, training workshops, serving on the EYES board and co-founding and co-chairing the NNF CBMR Metabolism Association for Postdocs and Students (MAPS). We are currently writing up the results for imminent submission for publication consideration.
