Periodic Reporting for period 1 - BioExtrusion (Natural functional plasticizers for controlled protein folding and extrusion into biomaterials.)
Periodo di rendicontazione: 2016-05-01 al 2018-04-30
In this project, one missing spinning parameter was investigated, namely small molecules, in the form of polyphenolic compounds that are co-extruded with the silk. We anticipate that small molecules assist in the silk alignment and may even induce β-sheet aggregation by removing the water necessary for converting the silk protein solution into a solid fibre during spinning.
The objectives of the project were twofold: (1) investigation of the polyphenol-induced conversion of proteins to β-sheet structures, necessary for a successful transformation of a protein solution into a solid material and (2) applying this knowledge for the cold extrusion of protein-based materials.
During the project, it was clear that the synthesis and purification of a small molecular compound accompanying the silk of the spider Nephila edulis was the key challenge of this project and required our full attention in order to study the small molecule-protein interactions. We successfully managed to produce this small molecule in large quantities and in its pure form, a major scientific breakthrough, paving the way for application-oriented scientific work. Furthermore, we found that this compound revealed antibacterial properties and therefore can be used as a new antibiotic. It is clear that this research opened many new possibilities and future perspectives, all which will be continued through continued research efforts beyond the Marie Curie fellowship.
The main results of the project include the establishment of:
- a new synthesis route for the small molecule found in the silk of Nehila edulis.
- a new purification protocol for the obtained molecule
- antibacterial tests using the small molecule
At present, IP protection is being sought for, whereafter the results of the project will be disseminated through various papers in high impact journals (Q1, 5% quartile).
Our initial data suggests that the general project hypothesis is very likely to be realistic. As a result, spinning at room temperature of not only proteins, but also alternative industrially relevant polymers will soon be in reach. Without any doubt, this will enable a new and real paradigm shift in the polymer processing industry.