Understanding the balance between functional and deleterious interactions of alpha-synuclein with lipid bilayers

Información del proyecto

toxiclipasyn

Identificador del acuerdo de subvención: 706551

DOI

10.3030/706551

Proyecto finalizado el 31 Marzo 2019

Fecha de la firma de la CE 8 Noviembre 2016

Fecha de inicio 1 Mayo 2017

Fecha de finalización 30 Abril 2019

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 171 460,80

Aportación de la UE

€ 171 460,80

Coordinado por

DEUTSCHES ZENTRUM FUR NEURODEGENERATIVE ERKRANKUNGEN EV
Germany

Objetivo

Proteins are among the most essential molecules of life. In order to fulfil their native function(s) they need to adopt a well-defined three-dimensional structure. However, under some circumstances, proteins can misfold and/or form toxic amyloid structures, which are the hallmark of a range of diseases including type 2 diabetes and neurodegenerative diseases. In particular, the small pre-synaptic protein, alpha-synuclein (AS), whose aggregation is the hallmark of Parkinson’s disease, can adopt in vivo and in vitro an intrinsically disordered conformation in solution and an alpha helical state when bound to membranes; the equilibrium between these two conformations has been shown to be important for its proposed native function, e.g. synaptic plasticity, and to modulate its kinetics of fibril formation.
Here, I propose to investigate the physiological factors responsible for the switch between functional and deleterious interactions between membrane bilayers and AS due to ageing or disease using an innovative combination of biological, structural, thermodynamic and kinetic studies. In particular, I propose to use both synthetic lipid model systems and isolated synaptic vesicles to study the effect of ageing and the presence of lipids associated with Parkinson’s disease pathology on the nature of the interaction between AS and lipid bilayers. The synaptic vesicles will be isolated from the brain of mice at different ageing stages, as well as from mice carrying gene modifications or knock out related to PD. The interaction between AS and the vesicles will be studied using a range of biophysical techniques including circular dichroism, fluorescence and nuclear magnetic resonance spectroscopy, Atomic Force and Electron Microscopy.
The aim of this study is to establish a thorough understanding of the interplay between changes in lipid composition and increased propensity of protein aggregation.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › neurobiología
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas
• ciencias naturales › ciencias físicas › óptica › microscopía › electron microscopy
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › lípidos
• ciencias naturales › ciencias físicas › óptica › espectroscopia

Palabras clave

• alpha-synuclein
• kinetic analysis
• amyloid
• Parkinson's Disease
• lipid peroxidation
• cholesterol
• synaptic vesicles
• lipidomic

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

DEUTSCHES ZENTRUM FUR NEURODEGENERATIVE ERKRANKUNGEN EV

• Aportación neta de la UEn: € 171 460,80
• Dirección: VENUSBERG-CAMPUS 1/99 ; 53127 BONN ; Alemania
• Región: Nordrhein-Westfalen › Köln › Bonn, Kreisfreie Stadt
• Tipo de actividad: Research Organisations
• Coste total: € 171 460,80