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Predictive modelling of 3D genome topology during progressive stages of embryonic development

Objective

The mechanisms that regulate genome activation and gene expression occur within a complex three-dimensional (3D) architecture, that helps bring functional regulatory elements into spatial proximity. Although observed at individual loci, the general principles and dynamics of enhancer-promoter interactions remain very poorly understood. The proposed project will use chromosome conformation capture, Hi-C, to measure 3D chromatin interactions at a genome-wide scale across different stages of embryogenesis.
The project will build on the Furlong lab’s recent findings in genome topology. First, it will look at changes in chromosome conformation during the entire developmental time-span to determine if the stability of interactions observed during the early stages holds true to the end of embryogenesis. It will also compare high-resolution interaction frequencies in two different cells types (mesodermal and neuronal) during different stages of development. The project will consider the entire spectrum of possible interactions, as opposed to a narrow set of enhancers, and thereby yield the first high-resolution view of the overall topology of the Drosophila genome as it develops and the general ‘search-space’ of developmental enhancers. Second, the results will identify constitutive and dynamic chromatin interactions, revealing the extent to which enhancer-promoter interactions change between cell types as they transition from a multipotent state to a terminally differentiated tissue. Third, these data will be used to make an integrative predictive model of chromatin loop formation, which should yield mechanistic insights into how chromatin contacts are formed and predict their dynamic or constitutive behaviour.
Given that the general principles underlying genomic architecture are deeply conserved, the project should not only help to explain regulatory principles underlying Drosophila development, but also greatly enhance our understanding of general chromatin organization.

Fields of science (EuroSciVoc)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2015

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Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 171 460,80
Address
Meyerhofstrasse 1
69117 Heidelberg
Germany

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Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 171 460,80
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