Objective
The mechanisms that regulate genome activation and gene expression occur within a complex three-dimensional (3D) architecture, that helps bring functional regulatory elements into spatial proximity. Although observed at individual loci, the general principles and dynamics of enhancer-promoter interactions remain very poorly understood. The proposed project will use chromosome conformation capture, Hi-C, to measure 3D chromatin interactions at a genome-wide scale across different stages of embryogenesis.
The project will build on the Furlong lab’s recent findings in genome topology. First, it will look at changes in chromosome conformation during the entire developmental time-span to determine if the stability of interactions observed during the early stages holds true to the end of embryogenesis. It will also compare high-resolution interaction frequencies in two different cells types (mesodermal and neuronal) during different stages of development. The project will consider the entire spectrum of possible interactions, as opposed to a narrow set of enhancers, and thereby yield the first high-resolution view of the overall topology of the Drosophila genome as it develops and the general ‘search-space’ of developmental enhancers. Second, the results will identify constitutive and dynamic chromatin interactions, revealing the extent to which enhancer-promoter interactions change between cell types as they transition from a multipotent state to a terminally differentiated tissue. Third, these data will be used to make an integrative predictive model of chromatin loop formation, which should yield mechanistic insights into how chromatin contacts are formed and predict their dynamic or constitutive behaviour.
Given that the general principles underlying genomic architecture are deeply conserved, the project should not only help to explain regulatory principles underlying Drosophila development, but also greatly enhance our understanding of general chromatin organization.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences mathematics pure mathematics topology
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2015
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.