We published a feature article about “chemometers” and their applications that gives a very good overview of CHEMO-RISK and the diverse aspects and challenges addressed in subprojects A, B, C and D (Rojo-Nieto & Jahnke 2023, doi:
https://doi.org/10.1039/D2CC06882F(öffnet in neuem Fenster)).
In (A) we developed chemometers for lean biota tissues (Rojo-Nieto et al. 2019
https://doi.org/10.1016/j.chemosphere.2018.12.134(öffnet in neuem Fenster)) and water that were applied for multimedia sampling at the Swedish background site Lake Angen. We sampled a diverse set of fish species, mussels and crayfish as well as sediment across the lake and sampled freely dissolved chemicals from water using two silicone “chemometers”. We compared Csilicone⇌medium for all media to assess trophic magnification factors (TMFs) and observed explicit biomagnification of hydrophobic, persistent organic pollutants, interpreted using stable isotope ratios of nitrogen. However, compared to sediments and water, the biota showed underequilibration, which may result from fast-growing and short-lived algae at the bottom of the food chain being below equilibrium partitioning regarding sediment. Rojo-Nieto et al. manuscript; Wernicke et al. 2022a (
https://doi.org/10.1016/j.ecoenv.2022.113285)(öffnet in neuem Fenster); Wernicke et al. 2022b (
https://doi.org/10.1186/s12302-022-00644-w(öffnet in neuem Fenster)).
In (B) we developed chemometers to assess the internal exposure and effects in liver, kidney, brain and lipid tissue of diverse marine mammal species from the German North and Baltic Sea. The chemometer extracts were submitted to bioanalytical screening in four cellular reporter gene bioassays, with liver samples usually eliciting the strongest effects. Furthermore, the chemometer extracts were submitted to chemical screening using gas chromatographic separation and high-resolution mass spectrometric determination of about 70 compounds (GC/HRMS, Muz et al. 2020
https://doi.org/10.1021/acs.est.0c05537(öffnet in neuem Fenster)). We also characterized the influence of co-dosed lipids on the response of the bioassays. These data served to extend a model to correct data for the reduced bioavailability in the bioassay system. Reiter et al. 2019 (
https://doi.org/10.1021/acs.est.9b07850)(öffnet in neuem Fenster); Reiter et al. 2022 (
https://doi.org/10.1016/j.envint.2022.107337)(öffnet in neuem Fenster); Reiter et al. 2023 (
https://doi.org/10.1039/D3EM00033H(öffnet in neuem Fenster)).
In (C) we developed chemometer plasters for the chemicals eliminated via human skin. A “sandwich” experiment tested chemical transfer from a ”donor” to an ”acceptor”. We further assessed whether the uptake into the silicone occurred mainly from the skin or the surrounding air using activated carbon as shielding layer. A pilot study investigated exposure times of 1-5 days on the upper thigh, compared with established silicone wristbands and blood. A separate method using thermodesorption and GC-MS/MS was established for a set of relevant compounds. Then, 100 cohort study participants wore the optimized sampler format along with the established wristband format and donated a blood sample to compare the patterns and levels of pollutants. This high risk/high gain project is in manuscript form: Abel et al. manuscript a; Abel et al. in manuscript b.
In (D) we aimed for suspect and non-target profiling to (i) establish a multi-target method for 150 persistent organic chemicals (Muz et al. 2020
ii(öffnet in neuem Fenster) develop a non-discriminatory clean-up for silicone-based chemometer extracts (Muz et al. 2021
https://doi.org/10.1002/etc.5153(öffnet in neuem Fenster)) and (iii) screen chemometer samples for peak patterns of potentially problematic chemicals. Pattern analysis in ”chemometer” extracts of diverse fish species covering different trophic levels from a contaminated site (Muz et al. manuscript) and investigating the chemometer extracts from different tissues of marine mammals (Schacht et al. manuscript) has been performed to explore the pattern analysis. Furthermore, a method that allows for the challenging analysis of polychlorinated and polybrominated dibenzodioxins and furans in liver samples of deer from different German regions has been established.
