RAtionalising Membrane Protein crystallisation

Livrables

Management Deliverable D6.1: Appointment of a project manager at UGA.

Training Deliverable D56 12 PhD examinations completedPostponed reasons initialy month 48During the Covid19 situation and especially the lockdown all facilities and university labs were closed for several months Mars June in every country of Europe The consortium managed to carry on supervising the ESRs remotely and through regular meetings with ESRs trying to limit the impact of this exceptional situation on their thesis Thus they advised ESRs to continue working on their thesis from home focusing on data analysis and planning for publications Nevertheless for all ESRs the completion of their PhD work is dependent on lab work Results obtained from experiments are also crucial for publications As a result almost all the 12 ESRs will need one extra year to finish their PhD theses and complete their PhD examinations

Recruitment of all ESRs

Management Deliverable D6.2: Recruitment of all ESRs.

Updated outreach plan

Dissemination Deliverable D7.1: Updated outreach plan available.

A policy document about structuring training in structural biology in Europe for consideration by the SB

Training Deliverable D55 A policy document about structuring training in structural biology in Europe for consideration by the Supervisory Board Postponed reasons initialy month 48Given the Covid19 lockdown and the subsequent delay in completion of all other deliverables it makes sense to push back the deadline for this deliverable This will allow us time to make the most considered assessment of the training programme and put us in the best position to complete this policy document

Final report and case study on the RAMP website

Dissemination Deliverable D76 Final report and case study on the RAMP websitePostponed reasons initially month 48Same reasons as above D69

Publications

New Malonate-Derived Tetraglucoside Detergents for Membrane Protein Stability

Auteurs: Muhammad Ehsan, Satoshi Katsube, Cristina Cecchetti, Yang Du, Jonas S. Mortensen, Haoqing Wang, Andreas Nygaard, Lubna Ghani, Claus J. Loland, Brian K. Kobilka, Bernadette Byrne, Lan Guan, Pil Seok Chae
Publié dans: ACS Chemical Biology, Numéro 15/6, 2020, Page(s) 1697-1707, ISSN 1554-8929
Éditeur: American Chemical Society
DOI: 10.1021/acschembio.0c00316

3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow

Auteurs: Diana C. F. Monteiro, David von Stetten, Claudia Stohrer, Marta Sans, Arwen R. Pearson, Gianluca Santoni, Peter van der Linden, Martin Trebbin
Publié dans: IUCrJ, Numéro 7/2, 2020, Page(s) 207-219, ISSN 2052-2525
Éditeur: International Union of Crystallography Journal
DOI: 10.1107/s2052252519016865

Crystallization of Proteins on Chip by Microdialysis for <i>In Situ</i> X-ray Diffraction Studies

Auteurs: Sofia Jaho, Niels Junius, Franck Borel, Yoann Sallaz-Damaz, Jean-Baptiste Salmon, Monika Budayova-Spano
Publié dans: Journal of Visualized Experiments, Numéro 170, 2021, ISSN 1940-087X
Éditeur: MYJoVE Corporation
DOI: 10.3791/61660

Pendant-bearing glucose-neopentyl glycol (P-GNG) amphiphiles for membrane protein manipulation: Importance of detergent pendant chain for protein stabilization

Auteurs: Hyoung Eun Bae, Cristina Cecchetti, Yang Du, Satoshi Katsube, Jonas S. Mortensen, Weijiao Huang, Shahid Rehan, Ho Jin Lee, Claus J. Loland, Lan Guan, Brian K. Kobilka, Bernadette Byrne, Pil Seok Chae
Publié dans: Acta Biomaterialia, Numéro 112, 2020, Page(s) 250-261, ISSN 1742-7061
Éditeur: Elsevier BV
DOI: 10.1016/j.actbio.2020.06.001

Homogeneous batch micro-crystallization of proteins from ammonium sulfate

Auteurs: Claudia Stohrer, Sam Horrell, Susanne Meier, Marta Sans, David von Stetten, Michael Hough, Adrian Goldman, Diana C. F. Monteiro, Arwen R. Pearson
Publié dans: Acta Crystallographica Section D Structural Biology, Numéro 77/2, 2021, Page(s) 194-204, ISSN 2059-7983
Éditeur: International Union of Crystallography/Wiley
DOI: 10.1107/s2059798320015454

Optimization of crystallization of biological macromolecules using dialysis combined with temperature control

Auteurs: Niels Junius, Elham Vahdatahar, Esko Oksanen, Jean-Luc Ferrer, Monika Budayova-Spano
Publié dans: Journal of Applied Crystallography, Numéro 53/3, 2020, Page(s) 686-698, ISSN 1600-5767
Éditeur: Journal of Applied Crystallography
DOI: 10.1107/s1600576720003209

Mixing Salts and Poly(ethylene glycol) into Protein Solutions: The Effects of Diffusion across Semipermeable Membranes and of Convection

Auteurs: Virginia Apostolopoulou, Niels Junius, Richard P. Sear, Monika Budayova-Spano
Publié dans: Crystal Growth & Design, Numéro 20/6, 2020, Page(s) 3927-3936, ISSN 1528-7483
Éditeur: American Chemical Society
DOI: 10.1021/acs.cgd.0c00246

Transfer of stabilising mutations between different secondary active transporter families

Auteurs: Cristina Cecchetti, Nicola J. Scull, Thotegowdanapalya C. Mohan, Yilmaz Alguel, Alexandra M. C. Jones, Alexander D. Cameron, Bernadette Byrne
Publié dans: FEBS Open Bio, Numéro 11/6, 2021, Page(s) 1685-1694, ISSN 2211-5463
Éditeur: Elsevier BV
DOI: 10.1002/2211-5463.13168

Membrane-protein crystals for neutron diffraction

Auteurs: Thomas Lykke-Møller Sørensen, Samuel John Hjorth-Jensen, Esko Oksanen, Jacob Lauwring Andersen, Claus Olesen, Jesper Vuust Møller, Poul Nissen
Publié dans: Acta Crystallographica Section D Structural Biology, Numéro 74/12, 2018, Page(s) 1208-1218, ISSN 2059-7983
Éditeur: Acta Crystallographica
DOI: 10.1107/s2059798318012561

A novel high-throughput screen for identifying lipids that stabilise membrane proteins in detergent based solution

Auteurs: Cristina Cecchetti, Jannik Strauss, Claudia Stohrer, Claire Naylor, Edward Pryor, Jeanette Hobbs, Simon Tanley, Adrian Goldman, Bernadette Byrne
Publié dans: PLOS ONE, Numéro 16/7, 2021, Page(s) e0254118, ISSN 1932-6203
Éditeur: Public Library of Science
DOI: 10.1371/journal.pone.0254118

Insights into the mechanism of high lipid–detergent crystallization of membrane proteins

Auteurs: S. Trampari, C. Neumann, S. J. Hjorth-Jensen, A. Shahsavar, E. M. Quistgaard and P. Nissen
Publié dans: Journal of Applied Crystallography, Numéro 54, 2021, Page(s) 1775-1783, ISSN 1600-5767
Éditeur: IUCr/Wiley
DOI: 10.1107/s1600576721010669

IMPROvER: the Integral Membrane Protein Stability Selector

Auteurs: Steven P. D. Harborne, Jannik Strauss, Jessica C. Boakes, Danielle L. Wright, James G. Henderson, Jacques Boivineau, Veli-Pekka Jaakola, Adrian Goldman
Publié dans: Scientific Reports, Numéro 10/1, 2020, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-020-71744-x

Transporter oligomerisation: roles in structure and function

Auteurs: Cristina Cecchetti, Euan Pyle, Bernadette Byrne
Publié dans: Biochemical Society Transactions, Numéro 47/1, 2019, Page(s) 433-440, ISSN 0300-5127
Éditeur: Portland Press, Ltd.
DOI: 10.1042/bst20180316

A microfluidic device for both on-chip dialysis protein crystallization and in situ X-ray diffraction

Auteurs: Niels Junius, Sofia Jaho, Yoann Sallaz-Damaz, Franck Borel, Jean-Baptiste Salmon, Monika Budayova-Spano
Publié dans: Lab on a Chip, Numéro 20/2, 2020, Page(s) 296-310, ISSN 1473-0197
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c9lc00651f

1,3,5-Triazine-Cored Maltoside Amphiphiles for Membrane Protein Extraction and Stabilization

Auteurs: Lubna Ghani, Chastine F. Munk, Xiang Zhang, Satoshi Katsube, Yang Du, Cristina Cecchetti, Weijiao Huang, Hyoung Eun Bae, Savvas Saouros, Muhammad Ehsan, Lan Guan, Xiangyu Liu, Claus J. Loland, Brian K. Kobilka, Bernadette Byrne, Pil Seok Chae
Publié dans: Journal of the American Chemical Society, Numéro 141/50, 2019, Page(s) 19677-19687, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.9b07883

Strategies for successful isolation of a eukaryotic transporter

Auteurs: Savvas Saouros, Cristina Cecchetti, Alex Jones, Alexander D. Cameron, Bernadette Byrne
Publié dans: Protein Expression and Purification, Numéro 166, 2020, Page(s) 105522, ISSN 1046-5928
Éditeur: Academic Press
DOI: 10.1016/j.pep.2019.105522

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Auteurs: Elham Vahdatahar, Niels Junius, Monika Budayova - Spano
Publié dans: Journal of Visualized Experiments, Numéro 169, 2021, ISSN 1940-087X
Éditeur: MYJoVE Corporation
DOI: 10.3791/61685

Maltose-bis(hydroxymethyl)phenol (MBPs) and maltose-tris(hydroxymethyl)phenol (MTPs) amphiphiles for Membrane protein stability.

Auteurs: Muhammad Ehsan, Haoqing Wang, Cristina Cecchetti, Jonas S. Mortensen, Yang Du, Parameswaran Hariharan, Ho Jin Lee, Lubna Ghani, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, and Pil Seok Chae
Publié dans: ACS Chemical Biology, Numéro 16/9, 2021, Page(s) 1779-1790, ISSN 1554-8929
Éditeur: American Chemical Society
DOI: 10.1021/acschembio.1c00578

A protocol for production of perdeuterated OmpF porin for neutron crystallography

Auteurs: Swati Aggarwal, Claes von Wachenfeldt, Suzanne Zoë Fisher, Esko Oksanen
Publié dans: Protein Expression and Purification - Elsevier, Numéro 188, 2021, ISSN 1046-5928
Éditeur: Academic Press
DOI: 10.1016/j.pep.2021.105954

Prospects for membrane protein crystals in NMX

Auteurs: Samuel John Hjorth-Jensen, Esko Oksanen, Poul Nissen, Thomas Lykke-Møller Sørensen
Publié dans: Neutron Crystallography in Structural Biology, Numéro 634, 2020, Page(s) 47-68, ISBN 9780128192146
Éditeur: Elsevier
DOI: 10.1016/bs.mie.2019.11.019

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