Periodic Reporting for period 2 - CATCH-U-DNA (Capturing non-Amplified Tumor Circulating DNA with Ultrasound Hydrodynamics)
Periodo di rendicontazione: 2018-06-01 al 2020-11-30
Regarding the technology, CATCH-U-DNA project produced a new detection platform (AWS) for the simultaneous real-time probing of a several samples using a 24-array biochip. Moreover, the implementation of a new generation of microfluidic fluidized bed in plasma and serum was demonstrated (Curie), although further development is necessary to reach the desirable detection limits with the acoustic-based detection platform for direct implementation in liquid-biopsy. Finally, the new microfluidic chip developed by JOBST-partner has been tested successfully with the new array biochip during immuno-detection. The chip is incorporated in the final platform and used for multisample analysis in cancer-diagnostics and beyond.
New knowledge was produced in acoustic biosensing, both technologically and conceptually. We showed that acoustic energy dissipation was more sensitive than frequency and could be exploited for ultra-sensitive DNA detection (FORTH). Systematic study of a huge range of liposomes (FORTH, BGU) revealed the ability of acoustic sensors to experimentally determine a quantitative correlation between the acoustic ratio and the liposomes’ mechanical properties (FORTH). Modeling/theory produced two new approaches for the study of physics underlying the acoustic detection mechanisms: one has extended/verified an older systematic study (FORTH) and the other revealing a completely new one (UAM); these works shed light on the major importance of hydrodynamics in acoustic sensor measurements, a parameter whose role and potential contributions is poorly debated (but largely ignored) in the bioacoustics community. Last, the development of colorimetric dendrimers and polydiacetylene nanoparticles (BGU) has contributed to the production of novel biomimetic nano-structures of potential interest to the screening of membrane-active peptides.
Regarding innovation, a novel detection platform and acoustic 24-array biochip is a significant technological output.A novel molecular diagnostic device for the point of care was further developed by FORTH partner. Other notable innovations include a reusable microfluidic chip for QCM-device multisensing (JOBST). Curie partner also advanced considerably their microfluidic fluidized bed for crude-sample pretreatment application, a technology currently exploited by a spin off company. Last, innovation has been produced related to the delivery of a software predictive tool based on UAM-partner’s software for QCM analysis of discrete soft matter analytes.
Finally, the project has a significant output in terms of dissemination activities and training of your students. We report, so far, 11 scientific publications in peer reviewed journals with several more under submission/preparation (>6), 2 new patents and over 30 activities for disseminations of the project results to the wide audience and specific target groups. We are also proud to produce a leading to products very close or close to the market, depending on future investment.
Regarding CATCH-U-DNA’s impact to clinical practice, healthcare and the society in general, the application-area of the new concept, i.e. the development of a DNA-based diagnostic system for cancer in serum, is still relevant and of immense significance to citizens’ well-being and healthcare industry’s interests. The market for clinical diagnostic and point-of-care tests is still worldwide one of the fastest-growing sectors, currently dominated by the USA; the area of integrated DNA analyzers alone is valued 35 billion € in 2015. CATCH-U-DNA concept is a very attractive one for healthcare industry since it proposes to replace PCR-based diagnostic approaches with a non-polymerase DNA amplification method. The elimination of cumbersome, expensive, time consuming and occasionally scientifically-biased procedures are some of our proposed method’s strong competitive advantages; these advantages are still relevant and some already demonstrated. Indeed, our work until today indicates that our ambitious goal to reach the necessary sensitivity of 100-1000 molecules in 1 ml of sample is feasible (WP4). Moreover, demonstrating the operation of the full system by the end of the project will be critical for the future commercial uptake and exploitation of CATCH-U-DNA. Specifically, bringing the system after three years of work to a TRL level 4 or even 5 through the contribution of the clinical partner to real-world validation will enhance significantly the commercial potential of the work. In this case, we hope our project to become a clinical as well as a commercial success. Overall, the project’s initial aim to contribute to improved healthcare, bringing at the same time an economic gain to involved partners is still very relevant.
In addition to the above, the new tools and technologies developed within the project are expected to have an impact in the development of molecular diagnostic platforms for the point of care and for the detection of infectious diseases and as a companion diagnostic tool (caner mutations, pharmacogenomics).