Objective
Recent advances in technologies to measure Rho GTPase signaling with unprecedented spatiotemporal resolution in single living cells have led to novel questions. Important new insights are that Rho GTPase signaling involves the formation of signaling complexes that fluctuate on subminute time and micrometer length scales, and that complex signaling networks involving multiple Rho GTPases fine tune the leading edge dynamics that power fibroblast migration. Novel methodologies are required to dissect this newly discovered signaling complexity. RhoSNATCH aims to characterize these signaling networks by acutely perturbing and recording Rho GTPase signaling at biologically relevant timescales in live-cell imaging experiments of cell migration. I will identify the crosstalk between Rac1/RhoA/Cdc42 that positions and maintains specific Rho GTPase activity zones in time and space and identify dedicated functional signaling modules that fine tune specific cytoskeletal output at the leading edge of motile fibroblasts.
Microfluidic technology will be used to induce precise fibroblast leading edge signaling states. Genome editing in combination with a reversible chemical dimerizing system will be used to acutely perturb endogenous RhoA,Cdc42 and Rac1 signaling, as well as a subset of their upstream regulators that regulate leading edge dynamics. Rho GTPase activation patterns and downstream cytoskeletal outputs will be systematically recorded in the different perturbed states. Computer-vision assisted image analysis will allow the multiplexing of multiple data sets to produce one of the first integrated models of the molecular circuitry that fine-tunes Rho GTPase signaling and cytoskeletal dynamics during leading edge extension. This novel, integrated approach will allow for the first time the identification of different spatiotemporally organised Rho GTPase signaling modules, and give relevant insight into the crosstalk between and functions of Rho GTPases in time and space.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors biosensors
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences physical sciences optics microscopy super resolution microscopy
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
3012 BERN
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.