Analytics for Biologics

Informations projet

A4B

N° de convention de subvention: 765502

DOI

10.3030/765502

Projet clôturé

Date de signature de la CE 29 Août 2017

Date de début 1 Octobre 2017

Date de fin 31 Mars 2022

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total

€ 3 933 617,40

Contribution de l’UE

€ 3 933 617,40

Coordonné par

UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF
Germany

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Other (3)

Participation in European Researchers' Night 1

For presenting the A4B ITN in public it A4B participants will participate in European Researchers Nights

Publication of advertising for open ESR positions

For acquiring the ESR advertising for open positions will be published in different media.

Participation in European Researchers' Night 2

For presenting the A4B ITN in public it A4B participants will participate in European Researchers Nights

Websites, patent fillings, videos etc. (1)

Project Website

Quotations about the preparation of the Website and their maintenance will be defined and applied. The content of the Website will defined at the kick-off meeting.

Publications

Peer reviewed articles (32)

Glycoform analysis of intact erythropoietin by MALDI FT-ICR mass spectrometry

Auteurs: Steffen Lippold, Raashina Thavarajah, Dietmar Reusch, Manfred Wuhrer, Simone Nicolardi
Publié dans: Analytica chimica acta, 2021, ISSN 0003-2670
Éditeur: Elsevier BV
DOI: 10.1016/j.aca.2021.339084

Fc gamma receptor IIIb binding of individual antibody proteoforms resolved by affinity chromatography–mass spectrometry

Auteurs: Steffen Lippold , Alexander Knaupp , Arnoud H de Ru , Rayman T N Tjokrodirijo , Peter A van Veelen, Erwin van Puijenbroek, Steven W de Taeye, Dietmar Reusch, Gestur Vidarsson, Manfred Wuhrer, Tilman Schlothauer, David Falck
Publié dans: mAbs, 2021, ISSN 1942-0870
Éditeur: Landes Bioscience
DOI: 10.1080/19420862.2021.1982847

FLASHIda enables intelligent data acquisition for top–down proteomics to boost proteoform identification counts

Auteurs: Kyowon Jeong, Maša Babović, Vladimir Gorshkov, Jihyung Kim, Ole N. Jensen, Oliver Kohlbacher
Publié dans: Nature Communications, 2022, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-022-31922-z

Intact and subunit-specific analysis of bispecific antibodies by sheathless CE-MS

Auteurs: Christoph Gstöttner, Simone Nicolardi, Markus Haberger, Dietmar Reusch, Manfred Wuhrer, Elena Domínguez-Vega
Publié dans: Analytica chimica acta, 2020, ISSN 0003-2670
Éditeur: Elsevier BV
DOI: 10.1016/j.aca.2020.07.069

Change of charge variant composition of trastuzumab upon stressing at physiological conditions

Auteurs: Baubek Spanov, Oladapo Olaleye, Nico Lingg, Arthur E.H. Bentlage, Natalia Govorukhina, Jos Hermans, Nico van de Merbel, Gestur Vidarsson, Alois Jungbauer, Rainer Bischoff
Publié dans: Journal of Chromatography A, Numéro 1655, 2021, Page(s) 462506, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2021.462506

Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol

Auteurs: Gregory Dutra, Daniel Komuczki, Alois Jungbauer, Peter Satzer
Publié dans: Engineering in Life Sciences, 2020, ISSN 1618-0240
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/elsc.201900160

Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells

Auteurs: Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer, Elena Domínguez-Vega
Publié dans: Analytical Chemistry, 2021, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.1c00893

Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation

Auteurs: Ramin Fazel, Yudong Guan, Behrouz Vaziri, Christoph Krisp, Laura Heikaus, Amirhossein Saadati, Siti Nurul Hidayah, Manasi Gaikwad, Hartmut Schlüter
Publié dans: Pharmaceuticals (Basel), 2019, ISSN 1424-8247
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph12010014

Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification

Auteurs: Steffen Lippold, Arnoud H de Ru, Jan Nouta, Peter A van Veelen, Magnus Palmblad, Manfred Wuhrer, Noortje de Haan
Publié dans: Beilstein journal of organic chemistry, 2020, ISSN 1860-5397
Éditeur: Beilstein-Institut
DOI: 10.3762/bjoc.16.253

Serum and Plasma Immunoglobulin G Fc N-Glycosylation Is Stable during Storage

Auteurs: Manuela Amez Martín, Manfred Wuhrer, David Falck
Publié dans: Journal of Proteome Research, 2021, ISSN 1535-3893
Éditeur: American Chemical Society
DOI: 10.1021/acs.jproteome.1c00148

Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics

Auteurs: Oksana Tyshchuk, Christoph Gstöttner, Dennis Funk, Simone Nicolardi, Stefan Frost, Stefan Klostermann, Tim Becker, Elena Jolkver, Felix Schumacher, Claudia Ferrara Koller, Hans Rainer Völger, Manfred Wuhrer, Patrick Bulau, and Michael Mølhøj
Publié dans: mAbs, 2019, ISSN 1942-0870
Éditeur: Landes Bioscience
DOI: 10.1080/19420862.2019.1635865

Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension

Auteurs: Emma von Witting, Sarah Lindbo, Magnus Lundqvist, Marit Möller, Andreas Wisniewski, Sara Kanje, Johan Rockberg, Hanna Tegel, Mikael Åstrand, Mathias Uhlen, and Sophia Hober
Publié dans: Molecular Pharmaceutics, 2021, ISSN 1543-8384
Éditeur: American Chemical Society
DOI: 10.1021/acs.molpharmaceut.0c00975

Cysteine Aminoethylation Enables the Site-Specific Glycosylation Analysis of Recombinant Human Erythropoietin using Trypsin

Auteurs: Steffen Lippold, Alexander Büttner, Matthew S F Choo, Michaela Hook, Coen J de Jong, Terry Nguyen-Khuong, Markus Haberger, Dietmar Reusch, Manfred Wuhrer, Noortje de Haan
Publié dans: Analytical chemistry, 2020, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.0c01794

Impact of failure rates, lot definitions and scheduling of upstream processes on the productivity of continuous integrated bioprocesses

Auteurs: Peter Satzer, Daniel Komuczki, Magdalena Pappenreiter, Alessandro Luigi Cataldo, Bernhard Sissolak, Alois Jungbauer
Publié dans: Journal of Chemical Technology & Biotechnology, 2020, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6648

An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass Spectrometry

Auteurs: Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voss, Ramin Fazel, Samira Ansari, Huali Shen, Jigang Wang, and Hartmut Schlüter
Publié dans: Journal of proteome research, 2021, ISSN 1535-3893
Éditeur: American Chemical Society
DOI: 10.1021/acs.jproteome.1c00221

Immunoglobulin G Glycoprofiles are Unaffected by Common Bottom-Up Sample Processing

Auteurs: Manuela Amez-Martín, Manfred Wuhrer, David Falck
Publié dans: Journal of Proteome Research, 2020, ISSN 1535-3893
Éditeur: American Chemical Society
DOI: 10.1021/acs.jproteome.0c00656

Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry

Auteurs: Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen, Thomas Kofoed
Publié dans: Amino Acids, 2021, ISSN 0939-4451
Éditeur: Springer Verlag
DOI: 10.1007/s00726-021-03004-9

Affinity capillary electrophoresis – mass spectrometry as tool to unravel proteoform-specific antibody-receptor interactions

Auteurs: Christoph Gstöttner, Michaela Hook, Tony Christopeit, Alexander Knaupp, Tilman Schlothauer, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, Elena Domínguez-Vega
Publié dans: Analytical Chemistry, 2021, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.1c03560

Effect of Trastuzumab-HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab

Auteurs: Baubek Spanov, Victoria Aboagye , Oladapo Olaleye, Natalia Govorukhina, Nico C van de Merbel, Rainer Bischoff
Publié dans: Frontiers in Chemistry, 2022, ISSN 2296-2646
Éditeur: Frontiers Media SA
DOI: 10.3389/fchem.2021.794247

FLASHDeconv: Ultrafast, high-quality feature deconvolution for top-down proteomics

Auteurs: Kyowon Jeong, Jihyung Kim, Manasi Gaikwad, Siti Nurul Hidayah, Laura Heikaus, Hartmut Schlüter, Oliver Kohlbacher
Publié dans: Cell Systems, 2020, ISSN 2405-4720
Éditeur: Cell Press
DOI: 10.1016/j.cels.2020.01.003

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

Auteurs: Steffen Lippold, Simone Nicolardi, Elena Domínguez-Vega, Anna-Katharina Heidenreich, Gestur Vidarsson, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, David Falck
Publié dans: mAbs, Numéro 11/7, 2019, Page(s) 1191-1196, ISSN 1942-0870
Éditeur: Landes Bioscience
DOI: 10.1080/19420862.2019.1636602

Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties

Auteurs: Steffen Lippold, Simone Nicolardi, Manfred Wuhrer, David Falck
Publié dans: Frontiers in Chemistry, Numéro 7, 2019, Page(s) 1-10, ISSN 2296-2646
Éditeur: Frontiers in Chemistry
DOI: 10.3389/fchem.2019.00698

Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry

Auteurs: Christoph Gstöttner, Dietmar Reusch, Markus Haberger, Irina Dragan, Peter van Veelen, David P. A. Kilgour, Yury O. Tsybin, Yuri E.M. van der Burgt, Manfred Wuhrer, Simone Nicolardi
Publié dans: mAbs, 2019, ISSN 1942-0870
Éditeur: Landes Bioscience
DOI: 10.1080/19420862.2019.1682403

Intact protein bioanalysis by liquid chromatography – High-resolution mass spectrometry

Auteurs: Peter Bults, Baubek Spanov, Oladapo Olaleye, Nico C. van de Merbel, Rainer Bischoff
Publié dans: Journal of Chromatography B, Numéro 1110-1111, 2019, Page(s) 155-167, ISSN 1570-0232
Éditeur: Elsevier BV
DOI: 10.1016/j.jchromb.2019.01.032

In-situ gradient formation by direct solid addition of buffer components

Auteurs: D.Komuczki, N.Lingg, A.Jungbauer, P.Satzer
Publié dans: Journal of Chromatography A, 2020, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2020.461663

Sheathless CE‐MS as a tool for monitoring exchange efficiency and stability of bispecific antibodies

Auteurs: Christoph Gstöttner, Dana L E Vergoossen, Manfred Wuhrer, Maartje G M Huijbers, Elena Domínguez-Vega
Publié dans: Electrophoresis, 2021, ISSN 0173-0835
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/elps.202000166

Anion exchange chromatography – Mass spectrometry for monitoring multiple quality attributes of erythropoietin biopharmaceuticals

Auteurs: Guusje van Schaick, Christoph Gstöttner, Alexander Büttner, Dietmar Reusch, Manfred Wuhrer, Elena Domínguez-Vega
Publié dans: Analytica chimica acta, 2021, ISSN 0003-2670
Éditeur: Elsevier BV
DOI: 10.1016/j.aca.2020.11.027

Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry

Auteurs: Oladapo Olaleye, Natalia Govorukhina, Nico C van de Merbel, Rainer Bischoff
Publié dans: Biomolecules, 2021, ISSN 2218-273X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11121791

Media on-demand: Continuous reconstitution of a chemically defined media directly from solids

Auteurs: Daniel Komuczki, Gregory Dutra, Christoph Gstöttner, Elena Dominguez-Vega, Alois Jungbauer, Peter Satzer
Publié dans: Biotechnology and Bioengineering, 2021, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27738

Enrichment and Liquid Chromatography–Mass Spectrometry Analysis of Trastuzumab and Pertuzumab Using Affimer Reagents

Auteurs: Oladapo Olaleye, Baubek Spanov, Robert Ford, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Publié dans: Analytical Chemistry, Numéro 93/40, 2021, Page(s) 13597-13605, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.1c02807

Analytical tools for the characterization of deamidation in monoclonal antibodies

Auteurs: Baubek Spanov, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Publié dans: Journal of Chromatography Open, 2022, ISSN 2772-3917
Éditeur: Elsevier B.V.
DOI: 10.1016/j.jcoa.2021.100025

Comparison of LFQ and IPTL for Protein Identification and Relative Quantification

Auteurs: Christina Johannsen, Christian J. Koehler, Bernd Thiede
Publié dans: Molecules, 2021, ISSN 1420-3049
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26175330

Book chapters (1)

Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry

Auteurs: Siti Nurul Hidayah, Manasi Gaikwad, Laura Heikaus, Hartmut Schlüter
Publié dans: Proteoforms [Working Title], 2020
Éditeur: IntechOpen
DOI: 10.5772/intechopen.89644