Analytics for Biologics

Risultati finali

Participation in European Researchers' Night 1

For presenting the A4B ITN in public it A4B participants will participate in European Researchers Nights

Publication of advertising for open ESR positions

For acquiring the ESR advertising for open positions will be published in different media.

Participation in European Researchers' Night 2

For presenting the A4B ITN in public it A4B participants will participate in European Researchers Nights

Quotations about the preparation of the Website and their maintenance will be defined and applied. The content of the Website will defined at the kick-off meeting.

Pubblicazioni

Glycoform analysis of intact erythropoietin by MALDI FT-ICR mass spectrometry

Autori: Steffen Lippold, Raashina Thavarajah, Dietmar Reusch, Manfred Wuhrer, Simone Nicolardi
Pubblicato in: Analytica chimica acta, 2021, ISSN 0003-2670
Editore: Elsevier BV
DOI: 10.1016/j.aca.2021.339084

Fc gamma receptor IIIb binding of individual antibody proteoforms resolved by affinity chromatography–mass spectrometry

Autori: Steffen Lippold , Alexander Knaupp , Arnoud H de Ru , Rayman T N Tjokrodirijo , Peter A van Veelen, Erwin van Puijenbroek, Steven W de Taeye, Dietmar Reusch, Gestur Vidarsson, Manfred Wuhrer, Tilman Schlothauer, David Falck
Pubblicato in: mAbs, 2021, ISSN 1942-0870
Editore: Landes Bioscience
DOI: 10.1080/19420862.2021.1982847

FLASHIda enables intelligent data acquisition for top–down proteomics to boost proteoform identification counts

Autori: Kyowon Jeong, Maša Babović, Vladimir Gorshkov, Jihyung Kim, Ole N. Jensen, Oliver Kohlbacher
Pubblicato in: Nature Communications, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-31922-z

Intact and subunit-specific analysis of bispecific antibodies by sheathless CE-MS

Autori: Christoph Gstöttner, Simone Nicolardi, Markus Haberger, Dietmar Reusch, Manfred Wuhrer, Elena Domínguez-Vega
Pubblicato in: Analytica chimica acta, 2020, ISSN 0003-2670
Editore: Elsevier BV
DOI: 10.1016/j.aca.2020.07.069

Change of charge variant composition of trastuzumab upon stressing at physiological conditions

Autori: Baubek Spanov, Oladapo Olaleye, Nico Lingg, Arthur E.H. Bentlage, Natalia Govorukhina, Jos Hermans, Nico van de Merbel, Gestur Vidarsson, Alois Jungbauer, Rainer Bischoff
Pubblicato in: Journal of Chromatography A, Numero 1655, 2021, Pagina/e 462506, ISSN 0021-9673
Editore: Elsevier BV
DOI: 10.1016/j.chroma.2021.462506

Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol

Autori: Gregory Dutra, Daniel Komuczki, Alois Jungbauer, Peter Satzer
Pubblicato in: Engineering in Life Sciences, 2020, ISSN 1618-0240
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/elsc.201900160

Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells

Autori: Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer, Elena Domínguez-Vega
Pubblicato in: Analytical Chemistry, 2021, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.1c00893

Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation

Autori: Ramin Fazel, Yudong Guan, Behrouz Vaziri, Christoph Krisp, Laura Heikaus, Amirhossein Saadati, Siti Nurul Hidayah, Manasi Gaikwad, Hartmut Schlüter
Pubblicato in: Pharmaceuticals (Basel), 2019, ISSN 1424-8247
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph12010014

Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification

Autori: Steffen Lippold, Arnoud H de Ru, Jan Nouta, Peter A van Veelen, Magnus Palmblad, Manfred Wuhrer, Noortje de Haan
Pubblicato in: Beilstein journal of organic chemistry, 2020, ISSN 1860-5397
Editore: Beilstein-Institut
DOI: 10.3762/bjoc.16.253

Serum and Plasma Immunoglobulin G Fc N-Glycosylation Is Stable during Storage

Autori: Manuela Amez Martín, Manfred Wuhrer, David Falck
Pubblicato in: Journal of Proteome Research, 2021, ISSN 1535-3893
Editore: American Chemical Society
DOI: 10.1021/acs.jproteome.1c00148

Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics

Autori: Oksana Tyshchuk, Christoph Gstöttner, Dennis Funk, Simone Nicolardi, Stefan Frost, Stefan Klostermann, Tim Becker, Elena Jolkver, Felix Schumacher, Claudia Ferrara Koller, Hans Rainer Völger, Manfred Wuhrer, Patrick Bulau, and Michael Mølhøj
Pubblicato in: mAbs, 2019, ISSN 1942-0870
Editore: Landes Bioscience
DOI: 10.1080/19420862.2019.1635865

Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension

Autori: Emma von Witting, Sarah Lindbo, Magnus Lundqvist, Marit Möller, Andreas Wisniewski, Sara Kanje, Johan Rockberg, Hanna Tegel, Mikael Åstrand, Mathias Uhlen, and Sophia Hober
Pubblicato in: Molecular Pharmaceutics, 2021, ISSN 1543-8384
Editore: American Chemical Society
DOI: 10.1021/acs.molpharmaceut.0c00975

Cysteine Aminoethylation Enables the Site-Specific Glycosylation Analysis of Recombinant Human Erythropoietin using Trypsin

Autori: Steffen Lippold, Alexander Büttner, Matthew S F Choo, Michaela Hook, Coen J de Jong, Terry Nguyen-Khuong, Markus Haberger, Dietmar Reusch, Manfred Wuhrer, Noortje de Haan
Pubblicato in: Analytical chemistry, 2020, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.0c01794

Impact of failure rates, lot definitions and scheduling of upstream processes on the productivity of continuous integrated bioprocesses

Autori: Peter Satzer, Daniel Komuczki, Magdalena Pappenreiter, Alessandro Luigi Cataldo, Bernhard Sissolak, Alois Jungbauer
Pubblicato in: Journal of Chemical Technology & Biotechnology, 2020, ISSN 0268-2575
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6648

An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass Spectrometry

Autori: Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voss, Ramin Fazel, Samira Ansari, Huali Shen, Jigang Wang, and Hartmut Schlüter
Pubblicato in: Journal of proteome research, 2021, ISSN 1535-3893
Editore: American Chemical Society
DOI: 10.1021/acs.jproteome.1c00221

Immunoglobulin G Glycoprofiles are Unaffected by Common Bottom-Up Sample Processing

Autori: Manuela Amez-Martín, Manfred Wuhrer, David Falck
Pubblicato in: Journal of Proteome Research, 2020, ISSN 1535-3893
Editore: American Chemical Society
DOI: 10.1021/acs.jproteome.0c00656

Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry

Autori: Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen, Thomas Kofoed
Pubblicato in: Amino Acids, 2021, ISSN 0939-4451
Editore: Springer Verlag
DOI: 10.1007/s00726-021-03004-9

Affinity capillary electrophoresis – mass spectrometry as tool to unravel proteoform-specific antibody-receptor interactions

Autori: Christoph Gstöttner, Michaela Hook, Tony Christopeit, Alexander Knaupp, Tilman Schlothauer, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, Elena Domínguez-Vega
Pubblicato in: Analytical Chemistry, 2021, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.1c03560

Effect of Trastuzumab-HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab

Autori: Baubek Spanov, Victoria Aboagye , Oladapo Olaleye, Natalia Govorukhina, Nico C van de Merbel, Rainer Bischoff
Pubblicato in: Frontiers in Chemistry, 2022, ISSN 2296-2646
Editore: Frontiers Media SA
DOI: 10.3389/fchem.2021.794247

FLASHDeconv: Ultrafast, high-quality feature deconvolution for top-down proteomics

Autori: Kyowon Jeong, Jihyung Kim, Manasi Gaikwad, Siti Nurul Hidayah, Laura Heikaus, Hartmut Schlüter, Oliver Kohlbacher
Pubblicato in: Cell Systems, 2020, ISSN 2405-4720
Editore: Cell Press
DOI: 10.1016/j.cels.2020.01.003

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

Autori: Steffen Lippold, Simone Nicolardi, Elena Domínguez-Vega, Anna-Katharina Heidenreich, Gestur Vidarsson, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, David Falck
Pubblicato in: mAbs, Numero 11/7, 2019, Pagina/e 1191-1196, ISSN 1942-0870
Editore: Landes Bioscience
DOI: 10.1080/19420862.2019.1636602

Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties

Autori: Steffen Lippold, Simone Nicolardi, Manfred Wuhrer, David Falck
Pubblicato in: Frontiers in Chemistry, Numero 7, 2019, Pagina/e 1-10, ISSN 2296-2646
Editore: Frontiers in Chemistry
DOI: 10.3389/fchem.2019.00698

Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry

Autori: Christoph Gstöttner, Dietmar Reusch, Markus Haberger, Irina Dragan, Peter van Veelen, David P. A. Kilgour, Yury O. Tsybin, Yuri E.M. van der Burgt, Manfred Wuhrer, Simone Nicolardi
Pubblicato in: mAbs, 2019, ISSN 1942-0870
Editore: Landes Bioscience
DOI: 10.1080/19420862.2019.1682403

Intact protein bioanalysis by liquid chromatography – High-resolution mass spectrometry

Autori: Peter Bults, Baubek Spanov, Oladapo Olaleye, Nico C. van de Merbel, Rainer Bischoff
Pubblicato in: Journal of Chromatography B, Numero 1110-1111, 2019, Pagina/e 155-167, ISSN 1570-0232
Editore: Elsevier BV
DOI: 10.1016/j.jchromb.2019.01.032

In-situ gradient formation by direct solid addition of buffer components

Autori: D.Komuczki, N.Lingg, A.Jungbauer, P.Satzer
Pubblicato in: Journal of Chromatography A, 2020, ISSN 0021-9673
Editore: Elsevier BV
DOI: 10.1016/j.chroma.2020.461663

Sheathless CE‐MS as a tool for monitoring exchange efficiency and stability of bispecific antibodies

Autori: Christoph Gstöttner, Dana L E Vergoossen, Manfred Wuhrer, Maartje G M Huijbers, Elena Domínguez-Vega
Pubblicato in: Electrophoresis, 2021, ISSN 0173-0835
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/elps.202000166

Anion exchange chromatography – Mass spectrometry for monitoring multiple quality attributes of erythropoietin biopharmaceuticals

Autori: Guusje van Schaick, Christoph Gstöttner, Alexander Büttner, Dietmar Reusch, Manfred Wuhrer, Elena Domínguez-Vega
Pubblicato in: Analytica chimica acta, 2021, ISSN 0003-2670
Editore: Elsevier BV
DOI: 10.1016/j.aca.2020.11.027

Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry

Autori: Oladapo Olaleye, Natalia Govorukhina, Nico C van de Merbel, Rainer Bischoff
Pubblicato in: Biomolecules, 2021, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/biom11121791

Media on-demand: Continuous reconstitution of a chemically defined media directly from solids

Autori: Daniel Komuczki, Gregory Dutra, Christoph Gstöttner, Elena Dominguez-Vega, Alois Jungbauer, Peter Satzer
Pubblicato in: Biotechnology and Bioengineering, 2021, ISSN 0006-3592
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27738

Enrichment and Liquid Chromatography–Mass Spectrometry Analysis of Trastuzumab and Pertuzumab Using Affimer Reagents

Autori: Oladapo Olaleye, Baubek Spanov, Robert Ford, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Pubblicato in: Analytical Chemistry, Numero 93/40, 2021, Pagina/e 13597-13605, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.1c02807

Analytical tools for the characterization of deamidation in monoclonal antibodies

Autori: Baubek Spanov, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Pubblicato in: Journal of Chromatography Open, 2022, ISSN 2772-3917
Editore: Elsevier B.V.
DOI: 10.1016/j.jcoa.2021.100025

Comparison of LFQ and IPTL for Protein Identification and Relative Quantification

Autori: Christina Johannsen, Christian J. Koehler, Bernd Thiede
Pubblicato in: Molecules, 2021, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26175330

Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry

Autori: Siti Nurul Hidayah, Manasi Gaikwad, Laura Heikaus, Hartmut Schlüter
Pubblicato in: Proteoforms [Working Title], 2020
Editore: IntechOpen
DOI: 10.5772/intechopen.89644

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