Interdisciplinary training network on the purinergic P2X7 receptor to control neuroinflammation and hyperexcitability in brain diseases.

Project Information

PurinesDX

Grant agreement ID: 766124

DOI 10.3030/766124

Project closed

EC signature date 17 August 2017

Start date 1 September 2017

End date 31 August 2021

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 3 487 463,28

EU contribution € 3 487 463,28

Coordinated by

ROYAL COLLEGE OF SURGEONS IN IRELAND
Ireland

Deliverables

Documents, reports (18)

Report on gender equality in PurinesDX M48

Report on gender equality in PurinesDX

Network-wide training events M24

Workshop on “Clinical techniques and challenges in patient treatment – from Birth to Old Age” and “Good Clinical Practice” (3 days, open to scientific audience; ~ 100 participants); Workshop “Drug design – from Target genes to Medicine” (2 days, open to scientific audience; ~ 30 participants)

Network-wide training events M41

Third Annual Meeting (1 day, ESRs); Transferable skills VI “Career perspectives” (1 day, ESRs); Final network conference “International colloquium on P2X7Rs and their role in brain diseases” (2 days, open to general public, ~100 participants)

Report on communication and public engagement activities M36

Report on communication and public engagement activities

Network-wide training events M8

Introduction Programme: “Brain diseases and purinergic signalling” (2 days, open to scientific audience, ~100 participants); Transferable skills course I: “Essential Project Management” & “Innovative & Entrepreneurial Working Groups” (2 days, ESRs)

Network-wide training events M12

Workshop “Animal experimentation & behaviour assessment” (3 days, open to scientific audience, ~ 50 participants); Transferable skills course II: “Basic Research Skills & Introduction to web-based learning” (2 days, ESRs)

Launch of website, Twitter, Facebook, LinkedIn, GooglePlus

PurinesDX website and social media

Report on gender equality in PurinesDX M36

Report on gender equality in PurinesDX

Report on communication and public engagement activities M12

Report on communication and public engagement activities

Report on gender equality in PurinesDX M12

Report on gender equality in PurinesDX

Network-wide training events M36

Transferable Skills V Bootcamp “Commercialisation and industry engagement” (4 days, ESRs); Workshop “The utility of stem cell research and its potential use in the clinic” (1 day, open to scientific audience, ~50 participants)

Report on communication and public engagement activities M6

Report on communication and public engagement activities

Network-wide training events M30

Second Annual Meeting (1 day, data presentation); Workshop “Diagnostic devices: Lab bench to clinic” (2 days, open to scientific audience; ~30 participants); Research interaction day (1 day, ESRs); Transferable skills course IV “Development of medical devices” (1 day, ESRs)

Risk protocol M18

Risk protocol

Report on communication and public engagement activities M48

Report on communication and public engagement activities

Report on gender equality in PurinesDX M24

Report on gender equality in PurinesDX

Network-wide training events M18

First Annual Meeting (data presentation) (1 day, ESRs); Workshop: “In vivo imaging” (1 day, ESRs); Transferable skills course III: “Research Communication and Dissemination” (2 days, ESRs)

Report on communication and public engagement activities M24

Report on communication and public engagement activities

Other (7)

Dissemination diary and report of dissemination events M36

Dissemination diary and report of dissemination events

Dissemination diary and report of dissemination events M48

Dissemination diary and report of dissemination events

Thesis completion of ESRs

Thesis completion of ESRs hired through PurinesDX

Dissemination diary and report of dissemination events M24

Dissemination diary and report of dissemination events

Dissemination diary and report of dissemination events M12

Dissemination diary and report of dissemination events

Supervisory Board of the network

Supervisory Board members of the network

Recruitment completion and ESR contracts

Publications

Peer reviewed articles (18)

Elevated blood purine levels as a biomarker of seizures and epilepsy

Author(s): Edward Beamer, Austin Lacey, Mariana Alves, Giorgia Conte, Faming Tian, Laura Diego‐Garcia, Mohamed Khalil, Felix Rosenow, Norman Delanty, Nicholas Dale, Hany El‐Naggar, David C. Henshall, Tobias Engel
Published in: Epilepsia, Issue 62/3, 2021, Page(s) 817-828, ISSN 0013-9580
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/epi.16839

Synthesis and Pharmacological Evaluation of Novel Non-nucleotide Purine Derivatives as P2X7 Antagonists for the Treatment of Neuroinflammation

Author(s): Francesco Calzaferri, Paloma Narros-Fernández, Ricardo de Pascual, Antonio M.G. de Diego, Annette Nicke, Javier Egea, Antonio G. García, Cristóbal de los Ríos
Published in: Journal of Medicinal Chemistry, Issue 64/4, 2021, Page(s) 2272-2290, ISSN 0022-2623
Publisher: American Chemical Society
DOI: 10.1021/acs.jmedchem.0c02145

Polyadenylation of mRNA as a novel regulatory mechanism of gene expression in temporal lobe epilepsy

Author(s): Alberto Parras, Laura de Diego-Garcia, Mariana Alves, Edward Beamer, Giorgia Conte, Eva M Jimenez-Mateos, James Morgan, Ivana Ollà, Yasmina Hernandez-Santana, Norman Delanty, Michael A Farrell, Donncha F O’Brien, Alejandro Ocampo, David C Henshall, Raúl Méndez, José J Lucas, Tobias Engel
Published in: Brain, Issue 143/7, 2020, Page(s) 2139-2153, ISSN 0006-8950
Publisher: Oxford University Press
DOI: 10.1093/brain/awaa168

The purinergic P2X7 receptor as a potential drug target to combat neuroinflammation in neurodegenerative diseases

Author(s): Francesco Calzaferri, Cristina Ruiz‐Ruiz, Antonio M. G. Diego, Ricardo Pascual, Iago Méndez‐López, María F. Cano‐Abad, Victoria Maneu, Cristóbal Ríos, Luis Gandía, Antonio G. García
Published in: Medicinal Research Reviews, Issue 40/6, 2020, Page(s) 2427-2465, ISSN 0198-6325
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/med.21710

Nucleotides regulate the common molecular mechanisms that underlie neurodegenerative diseases; Therapeutic implications

Author(s): Álvaro Sebastián-Serrano, Laura de Diego-García, Caterina di Lauro, Carolina Bianchi, Miguel Díaz-Hernández
Published in: Brain Research Bulletin, Issue 151, 2019, Page(s) 84-91, ISSN 0361-9230
Publisher: Elsevier BV
DOI: 10.1016/j.brainresbull.2019.01.031

P2X7R antagonists in chronic stress-based depression models: a review

Author(s): Iven-Alex von Muecke-Heim, Clemens Ries, Lidia Urbina, Jan M. Deussing
Published in: European Archives of Psychiatry and Clinical Neuroscience, 2021, ISSN 0940-1334
Publisher: Dr. Dietrich Steinkopff Verlag
DOI: 10.1007/s00406-021-01306-3

P2X7 Receptor-Dependent Layer-Specific Changes in Neuron-Microglia Reactivity in the Prefrontal Cortex of a Phencyclidine Induced Mouse Model of Schizophrenia

Author(s): Stefano Calovi, Paula Mut-Arbona, Pál Tod, András Iring, Annette Nicke, Susana Mato, E. Sylvester Vizi, Jan Tønnesen, Beata Sperlagh
Published in: Frontiers in Molecular Neuroscience, Issue 13, 2020, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2020.566251

Deviant reporter expression and P2X4 passenger gene overexpression in the soluble EGFP BAC transgenic P2X7 reporter mouse model

Author(s): Antonio Ramírez-Fernández, Lidia Urbina-Treviño, Giorgia Conte, Mariana Alves, Björn Rissiek, Anna Durner, Nicolas Scalbert, Jiong Zhang, Tim Magnus, Friedrich Koch-Nolte, Nikolaus Plesnila, Jan M. Deussing, Tobias Engel, Robin Kopp, Annette Nicke
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-76428-0

Acupuncture alleviates acid‐ and purine‐induced pain in rodents

Author(s): Ying Zhang, Lumei Huang, Sergey A. Kozlov, Patrizia Rubini, Yong Tang, Peter Illes
Published in: British Journal of Pharmacology, Issue 177/1, 2020, Page(s) 77-92, ISSN 0007-1188
Publisher: Wiley-Blackwell
DOI: 10.1111/bph.14847

Chronic administration of P2X7 receptor antagonist JNJ-47965567 delays disease onset and progression, and improves motor performance in ALS SOD1G93A female mice

Author(s): Cristina Ruiz-Ruiz, Nuria García-Magro, Pilar Negredo, Carlos Avendaño, Anindya Bhattacharya, Marc Ceusters, Antonio G. García
Published in: Disease Models & Mechanisms, Issue 13/10, 2020, ISSN 1754-8403
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dmm.045732

Bi-directional genetic modulation of GSK-3β exacerbates hippocampal neuropathology in experimental status epilepticus

Author(s): Tobias Engel, Raquel Gómez-Sintes, Mariana Alves, Eva M. Jimenez-Mateos, Marta Fernández-Nogales, Amaya Sanz-Rodriguez, James Morgan, Edward Beamer, Alberto Rodríguez-Matellán, Mark Dunleavy, Takanori Sano, Jesus Avila, Miguel Medina, Felix Hernandez, José J. Lucas, David C. Henshall
Published in: Cell Death & Disease, Issue 9/10, 2018, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-018-0963-5

Amyloid Peptide Induced Neuroinflammation Increases the P2X7 Receptor Expression in Microglial Cells, Impacting on Its Functionality

Author(s): Carlos Martínez-Frailes, Caterina Di Lauro, Carolina Bianchi, Laura de Diego-García, Álvaro Sebastián-Serrano, Lisardo Boscá, Miguel Díaz-Hernández
Published in: Frontiers in Cellular Neuroscience, Issue 13, 2019, ISSN 1662-5102
Publisher: Frontiers Research Foundation
DOI: 10.3389/fncel.2019.00143

The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel

Author(s): Gabriele Stephan, Lumei Huang, Yong Tang, Sandra Vilotti, Elsa Fabbretti, Ye Yu, Wolfgang Nörenberg, Heike Franke, Flóra Gölöncsér, Beáta Sperlágh, Anke Dopychai, Ralf Hausmann, Günther Schmalzing, Patrizia Rubini, Peter Illes
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-03728-5

MicroRNA-22 Controls Aberrant Neurogenesis and Changes in Neuronal Morphology After Status Epilepticus

Author(s): Edward H. Beamer, Jeronimo Jurado-Arjona, Eva M. Jimenez-Mateos, James Morgan, Cristina R. Reschke, Aidan Kenny, Gioacchino de Leo, Luis A. Olivos-Oré, Marina Arribas-Blázquez, Stephen F. Madden, Jesús Merchán-Rubira, Norman Delanty, Michael A. Farrell, Donncha F. O’Brien, Jesus Avila, Miguel Diaz-Hernandez, M. Teresa Miras-Portugal, Antonio R. Artalejo, Felix Hernandez, David C. Henshall, T
Published in: Frontiers in Molecular Neuroscience, Issue 11, 2018, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2018.00442

The Role of P2X7 Receptor in Alzheimer’s Disease

Author(s): Linda Francistiová, Carolina Bianchi, Caterina Di Lauro, Álvaro Sebastián-Serrano, Laura de Diego-García, Julianna Kobolák, András Dinnyés, Miguel Díaz-Hernández
Published in: Frontiers in Molecular Neuroscience, Issue 13, 2020, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2020.00094

P2X7 Interactions and Signaling – Making Head or Tail of It

Author(s): Robin Kopp, Anna Krautloher, Antonio Ramírez-Fernández, Annette Nicke
Published in: Frontiers in Molecular Neuroscience, Issue 12, 2019, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2019.00183

P2X7 Receptor-Dependent microRNA Expression Profile in the Brain Following Status Epilepticus in Mice

Author(s): Giorgia Conte, Ngoc T. Nguyen, Mariana Alves, Laura de Diego-Garcia, Aidan Kenny, Annette Nicke, David C. Henshall, Eva M. Jimenez-Mateos, Tobias Engel
Published in: Frontiers in Molecular Neuroscience, Issue 13, 2020, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2020.00127

P2X7 Receptor Antagonism as a Potential Therapy in Amyotrophic Lateral Sclerosis

Author(s): Cristina Ruiz-Ruiz, Francesco Calzaferri, Antonio G. García
Published in: Frontiers in Molecular Neuroscience, Issue 13, 2020, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2020.00093