Periodic Reporting for period 3 - ChronosAntibiotics (Exploring the bacterial cell cycle to re-sensitize antibiotic-resistant bacteria)
Periodo di rendicontazione: 2021-03-01 al 2022-08-31
At the same time, we are developing S. aureus reporter strains for the identification of new antimicrobial compounds. These strains encode fluorescent proteins under the control of promoters that respond to the inhibition of specific metabolic pathways. We currently have reporter strains that detect inhibition of cell wall synthesis, DNA damage, loss of membrane potential and of fatty acids biosynthesis and are working towards increasing this list. These strains should be useful for whole-cell screens for new antibiotics, as well as to study the mode of action of new compounds with antimicrobial activity.