Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes

Project Information

SPICE

Grant agreement ID: 772027

DOI

10.3030/772027

Project closed

EC signature date 18 April 2018

Start date 1 June 2018

End date 30 November 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 988 697,50

EU contribution

€ 1 988 697,50

Coordinated by

UNIVERSITAT KONSTANZ
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (22)

Magnesium induced structural reorganization in the active site of adenylate kinase

Author(s): Kwangho Nam, Abdul Raafik Arattu Thodika, Sonja Tischlik, Chanrith Phoeurk, Tamás Milán Nagy, Léon Schierholz, Jörgen Ådén, Per Rogne, Malte Drescher, A. Elisabeth Sauer-Eriksson, Magnus Wolf-Watz
Published in: Science Advances, Issue 10, 2024, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.ado5504

Intracellular Protein–Lipid Interactions Studied by Rapid-Scan Electron Paramagnetic Resonance Spectroscopy

Author(s): Theresa S. Braun, Juliane Stehle, Sylwia Kacprzak, Patrick Carl, Peter Höfer, Vinod Subramaniam, Malte Drescher
Published in: The Journal of Physical Chemistry Letters, Issue 12, 2023, Page(s) 2471-2475, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.0c03583

Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

Author(s): Annalisa Pierro, Malte Drescher
Published in: Chemical Communications, Issue 59, 2024, Page(s) 1274-1284, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2cc05907j

Insights into Enzymatic Catalysis from Binding and Hydrolysis of Diadenosine Tetraphosphate by <i>E</i>. <i>coli</i> Adenylate Kinase

Author(s): Sonja Tischlik, Melanie Oelker, Per Rogne, A. Elisabeth Sauer-Eriksson, Malte Drescher, Magnus Wolf-Watz
Published in: Biochemistry, Issue 62, 2023, Page(s) 2238-2243, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.3c00189

Increasing the Modulation Depth of Gd^III-Based Pulsed Dipolar EPR Spectroscopy (PDS) with Porphyrin–Gd^III Laser-Induced Magnetic Dipole Spectroscopy

Author(s): Andreas Scherer, Xuemei Yao, Mian Qi, Max Wiedmaier, Adelheid Godt, Malte Drescher
Published in: The Journal of Physical Chemistry Letters, Issue 13, 2023, Page(s) 10958-10964, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.2c02138

Combining Site-Directed Spin Labeling in Vivo and In-Cell EPR Distance Determination

Author(s): Pia Widder, Julian Schuck, Daniel Summerer, Malte Drescher
Published in: PCCP, 2021, ISSN 1463-9076
Publisher: Royal Society of Chemistry
DOI: 10.26434/chemrxiv.9746222.v1

Background removal from rapid-scan EPR spectra of nitroxide-based spin labels by minimizing non-quadratic cost functions

Author(s): Florian Johannsen, Malte Drescher
Published in: Journal of Magnetic Resonance Open, Issue 16-17, 2023, Page(s) 100121, ISSN 2666-4410
Publisher: Elsevier BV
DOI: 10.1016/j.jmro.2023.100121

Optimising broadband pulses for DEER depends on concentration and distance range of interest

Author(s): Andreas Scherer, Sonja Tischlik, Sabrina Weickert, Valentin Wittmann, Malte Drescher
Published in: Magnetic Resonance, Issue 1, 2025, Page(s) 59-74, ISSN 2699-0016
Publisher: Copernicus GmbH
DOI: 10.5194/mr-1-59-2020

Isoindoline‐Based Nitroxides as Bioresistant Spin Labels for Protein Labeling through Cysteines and Alkyne‐Bearing Noncanonical Amino Acids

Author(s): Theresa Sophie Braun, Pia Widder, Uwe Osswald, Lina Groß, Lara Williams, Moritz Schmidt, Irina Helmle, Daniel Summerer, Malte Drescher
Published in: ChemBioChem, Issue 21, 2023, Page(s) 958-962, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201900537

The molecular mechanism of Hsp90-induced oligomerization of Tau

Author(s): Sabrina Weickert, Magdalena Wawrzyniuk, Laura John, Stefan G. D. Rüdiger, Malte Drescher
Published in: Science Advances, 2019, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1101/614289

Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy

Author(s): Pia Widder, Frederic Berner, Daniel Summerer, Malte Drescher
Published in: ACS Chemical Biology, Issue 14/5, 2019, Page(s) 839-844, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.8b01111

Gd(III)–Gd(III) Relaxation-Induced Dipolar Modulation Enhancement for In-Cell Electron Paramagnetic Resonance Distance Determination

Author(s): Mykhailo Azarkh, Anna Bieber, Mian Qi, Jörg W. A. Fischer, Maxim Yulikov, Adelheid Godt, Malte Drescher
Published in: The Journal of Physical Chemistry Letters, Issue 10/7, 2019, Page(s) 1477-1481, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.9b00340

Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry

Author(s): Anandi Kugele, Bjarne Silkenath, Jakob Langer, Valentin Wittmann, Malte Drescher
Published in: ChemBioChem, 2019, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201900318

Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid

Author(s): Anandi Kugele, Theresa Sophie Braun, Pia Widder, Lara Williams, Moritz Johannes Schmidt, Daniel Summerer, Malte Drescher
Published in: Chemical Communications, Issue 55/13, 2019, Page(s) 1923-1926, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc09325c

Expanding the Genetic Code for Site-Directed Spin-Labeling

Author(s): Theresa Braun, Malte Drescher, Daniel Summerer
Published in: International Journal of Molecular Sciences, Issue 20/2, 2019, Page(s) 373, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20020373

Light-induced dipolar spectroscopy – A quantitative comparison between LiDEER and LaserIMD

Author(s): Anna Bieber, Dennis Bücker, Malte Drescher
Published in: Journal of Magnetic Resonance, Issue 296, 2018, Page(s) 29-35, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2018.08.006

Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy

Author(s): Lara Williams, Sonja Tischlik, Andreas Scherer, Jörg Wolfram Anselm Fischer, Malte Drescher
Published in: Chemical Communications, Issue 56, 2024, Page(s) 14669-14672, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc03101a

Rapid Scan Electron Paramagnetic Resonance Spectroscopy Is a Suitable Tool to Study Intermolecular Interactions of Intrinsically Disordered Protein

Author(s): Jessica Dröden, Malte Drescher
Published in: Biology, Issue 12, 2024, Page(s) 79, ISSN 2079-7737
Publisher: MDPI AG
DOI: 10.3390/biology12010079

<i>In situ</i> EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

Author(s): Anandi Kugele, Sophie Ketter, Bjarne Silkenath, Valentin Wittmann, Benesh Joseph, Malte Drescher
Published in: Chemical Communications, Issue 57, 2024, Page(s) 12980-12983, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cc04612h

Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation

Author(s): Niccolò Mosesso, Niharika Savant Lerner, Tobias Bläske, Felix Groh, Shane Maguire, Marie Laura Niedermeier, Eliane Landwehr, Karin Vogel, Konstanze Meergans, Marie-Kristin Nagel, Malte Drescher, Florian Stengel, Karin Hauser, Erika Isono
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-49485-6

The effect of the zero-field splitting in light-induced pulsed dipolar electron paramagnetic resonance (EPR) spectroscopy

Author(s): Andreas Scherer, Berk Yildirim, Malte Drescher
Published in: Magnetic Resonance, Issue 4, 2025, Page(s) 27-46, ISSN 2699-0016
Publisher: Copernicus GmbH
DOI: 10.5194/mr-4-27-2023

Real-time Monitoring of Photoinduced pH-jumps by In Situ Rapid-scan EPR Spectroscopy

Author(s): Florian Johannsen, Lara Williams, Man Him Chak, Malte Drescher
Published in: Journal of Physical Chemistry Letters, 2023, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.26434/chemrxiv-2023-x6d2t

Other (1)

Spin labelling via metabolic glycoengineering for studying post-translational protein modification by electron paramagnetic resonance spectroscopy

Author(s): Anna Rubailo, Valentin Wittmann, Malte Drescher
Published in: Chemrxiv, 2022
Publisher: chemrxiv
DOI: 10.26434/chemrxiv-2022-j2mc1-v2