Building the product pipeline for commercial demonstration of Plant Molecular Factories

Información del proyecto

Pharma-Factory

Identificador del acuerdo de subvención: 774078

DOI

10.3030/774078

Proyecto cerrado

Fecha de la firma de la CE 6 Octubre 2017

Fecha de inicio 1 Noviembre 2017

Fecha de finalización 31 Agosto 2022

Financiado con arreglo a

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Coste total

€ 9 254 335,00

Aportación de la UE

€ 8 286 008,63

Coordinado por

ST GEORGE'S HOSPITAL MEDICAL SCHOOL
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (1)

GMP guidance

Guidance on GMP compliance in Plant Molecular Farming

Demonstrators, pilots, prototypes (2)

Marketed kit

Recombinant protein kit placed on market

Diagnostic kit for sero-negative RA on the market

Other (1)

Public exhibits for PMF

3 public exhibits

Websites, patent fillings, videos etc. (2)

Project website

Public and project website

Social media and PMF website established

Social media strategies and setup of an overarching public website for Plant Molecular Farming (not to be confused with the project website).

Publicaciones

Peer reviewed articles (35)

Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process

Autores: Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Jonas de Groot, Jean-Pierre Ele-Ekouna, Marina Guillet, Florian Cardon, Anneli Ritala
Publicado en: Biotechnology and Bioengineering, Edición 00063592, 2022, Página(s) 2831-2841, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28178

Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

Autores: Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, Carlos Afonso, Patrice Lerouge, Muriel Bardor
Publicado en: Carbohydrate Polymers, Edición 259, 2021, Página(s) 117660, ISSN 0144-8617
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2021.117660

A simplified techno‐economic model for the molecular pharming of antibodies

Autores: Pere Mir‐Artigues, Richard M. Twyman, Derry Alvarez, Pedro Cerda Bennasser, Merce Balcells, Paul Christou, Teresa Capell
Publicado en: Biotechnology and Bioengineering, Edición 116/10, 2019, Página(s) 2526-2539, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27093

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

Autores: Elodie Mathieu-Rivet, Narimane Mati-Baouche, Marie-Laure Walet-Balieu, Patrice Lerouge, Muriel Bardor
Publicado en: Frontiers in Plant Science, Edición 11, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.609993

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Autores: Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Marina Guillet, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Florian Cardon
Publicado en: Frontiers in Plant Science, Edición 11, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.00033

The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use—A journey through a forest of regulatory and legal implications

Autores: Holger Spiegel, Greta Nölke, Harry Thangaraj and Stefan Schillberg
Publicado en: Frontiers in Bioengineering and Biotechnology, Edición 22964185, 2022, ISSN 2296-4185
Editor: Frontiers SA
DOI: 10.3389/fbioe.2022.926239

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Autores: Borja Diego-Martin, Beatriz González, Marta Vazquez-Vilar, Sara Selma, Rubén Mateos-Fernández, Silvia Gianoglio, Asun Fernández-del-Carmen, Diego Orzáez
Publicado en: Frontiers in Plant Science, Edición 11, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.612781

Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics

Autores: Hadrien Peyret, Elisabetta Groppelli, David Clark, Nicholas Eckersley, Tim Planche, Julian Ma, George P. Lomonossoff
Publicado en: Journal of Virological Methods, Edición 01660934, 2022, ISSN 0166-0934
Editor: Elsevier BV
DOI: 10.1016/j.jviromet.2021.114372

Plant Molecular Farming as a Strategy Against COVID-19 - The Italian Perspective

Autores: Chiara Lico; Luca Santi; Selene Baschieri; Emanuela Noris; Carla Marusic; Marcello Donini; Emanuela Pedrazzini; Giovanni Maga; Rosella Franconi; Paola Di Bonito; Linda Avesani
Publicado en: Frontiers in plant science 11 (2020). doi:10.3389/fpls.2020.609910, Edición 2, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.609910

Physicochemical characterization of the recombinant lectin scytovirin and microbicidal activity of the SD1 domain produced in rice against HIV‑1

Autores: Victoria Armario-Najera, Amaya Blanco-Perera, Shilpa R. Shenoy, Yi Sun, Silvia Marfil, Jordana Muñoz-Basagoiti, Daniel Perez-Zsolt, Julià Blanco, Nuria Izquierdo-Useros, Teresa Capell, Barry R. O’Keefe & Paul Christou
Publicado en: Plant Cell Reports, 2022, Página(s) 1013-1023, ISSN 0721-7714
Editor: Springer Verlag
DOI: 10.1007/s00299-022-02834-5

Investigation of a monoclonal antibody against enterotoxigenic Escherichia coli, expressed as secretory IgA1 and IgA2 in plants

Autores: Audrey Y-H Teh,Lisa Cavacini,Yue Hu,Ozan S. Kumru,Jian Xiong,David T. BolickORCID Icon,Sangeeta B. Joshi,Clemens Grünwald-Gruber,Friedrich Altmann,Mark Klempner,Richard L. Guerrant,David B. Volkin, Yang Wang and Julian K-C. Ma
Publicado en: Gut Microbes, Edición volume 13 issue 1, 2021, Página(s) 1-14, ISSN 1949-0984
Editor: Taylor Francis online
DOI: 10.1080/19490976.2020.1859813

A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

Autores: Eva C. Thuenemann, Matthew J. Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells ,Inmaculada Ferriol, Mattia Santoni, John F. C. Steele, Neil A. Ranson, Linda Avesani, Juan Jose Lopez-Moya and George P. Lomonossoff
Publicado en: Viruses, 2021, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13050885

Plant Virus Nanoparticles for Vaccine Applications

Autores: Mattia Santoni, Roberta Zampieri, Linda Avesani
Publicado en: Current Protein & Peptide Science, Edición 21/4, 2020, Página(s) 344-356, ISSN 1389-2037
Editor: Bentham Science Publishers
DOI: 10.2174/1389203721666200212100255

Multiple gene expression in plants using MIDAS-P, a versatile type II restriction-based modular expression vector.

Autores: Elizabeth C. Pinneh, Craig J. van Dolleweerd, Kathrin Göritzer, Pascal M. W. Drake, Julian K-C. Ma, Audrey Y-H. Teh
Publicado en: Biotechnology and Bioengineering, 2022, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28073

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Autores: Norma Aliaga-Franco1, Cunjin Zhang2, Silvia Presa1, Anjil K. Srivastava2, Antonio Granell1, David Alabadí1, Ari Sadanandom2, Miguel A. Blázquez1 and Eugenio G. Minguet1*
Publicado en: Fronteirs in Plant Science, 2019, ISSN 1664-462x
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.01150

Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases

Autores: Lobato, Maria; Huang, Xin; Alvarez, Derry; He, Wenshu; Baysal, Can; Zhu, Changfu; Armario-Nájera, Victoria; Blanco Perera, Amaya; Cerda, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes F.; Cunha, Nicolau B. da; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyapp
Publicado en: Plant Biotechnology Journal, 2021, Página(s) 1901-1920, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13657

Microalgae as an Efficient Vehicle for the Production andTargeted Delivery of Therapeutic Glycoproteins againstSARS-CoV-2 Variants

Autores: Jaber Dehghani 1 , Ali Movafeghi 2 , Elodie Mathieu-Rivet 1, Narimane Mati-Baouche 1 , Sébastien Calbo 3 , Patrice Lerouge 1,† and Muriel Bardor
Publicado en: Marine Drugs, Edición 20, 657, 2022, ISSN 1660-3397
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/md20110657

Potential Applications of Plant Biotechnology against SARS-CoV-2

Autores: Teresa Capell, Richard M. Twyman, Victoria Armario-Najera, Julian K.-C. Ma, Stefan Schillberg, Paul Christou
Publicado en: Trends in Plant Science, Edición 25/7, 2020, Página(s) 635-643, ISSN 1360-1385
Editor: Elsevier BV
DOI: 10.1016/j.tplants.2020.04.009

Investigation of the N-Glycosylation of the SARS-CoV-2 S Protein Contained in VLPs Produced in Nicotiana benthamiana

Autores: Juliette Balieu, Jae-Wan Jung, Philippe Chan, George P. Lomonossoff, Patrice Lerouge, and Muriel Bardor
Publicado en: Molecules, Edición 27, 2022, Página(s) 5119, ISSN 1420-3049
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27165119

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Autores: Coralie Chuberre, Philippe Chan, Marie-Laure Walet-Balieu, François Thiébert, Carole Burel, Julie Hardouin, Bruno Gügi and Muriel Bardor
Publicado en: Frontiers in Plant Science, 2022, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.673113

Plant-based expression and characterization of SARS-CoV-2 virus-like particles presenting a native spike protein

Autores: Jae-Wan Jung, Gergana Zahmanova, Ivan Minkov, George P. Lomonossoff
Publicado en: Plant Biotechnology Journal, Edición 20, 2022, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13813

Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes

Autores: Charlotte Toustou, Marie-Laure Walet-Balieu, Marie-Christine Kiefer-Meyer, Marine Houdou, Patrice Lerouge, François Foulquier, Muriel Bardor
Publicado en: Biological Reviews, Edición 14647931, 2021, Página(s) 732-748, ISSN 1464-7931
Editor: Cambridge University Press
DOI: 10.1111/brv.12820

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products.

Autores: Jonathan Menary; Matthew Hobbs; Sara Mesquita de Albuquerque; Agata Pacho; Pascal M. W. Drake; Alison Prendiville; Julian K-C. Ma; Sebastian S. Fuller
Publicado en: PLos One, Edición 2, 2020, ISSN 1932-6203
Editor: Public Library of Science
DOI: 10.31235/osf.io/gwve2

The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization

Autores: Keith Saunders, Eva C. Thuenemann, Sachin N. Shah, Hadrien Peyret, Ruth Kristianingsih, Sergio G. Lopez, Jake Richardson and George P. Lomonossoff
Publicado en: Frontiers in Bioengineering and Biotechnology, Edición 22964185, 2022, ISSN 2296-4185
Editor: Frontiers SA
DOI: 10.3389/fbioe.2022.877361

Unexpected synergistic HIV neutralization by a triple microbicide produced in rice endosperm

Autores: Evangelia Vamvaka, Gemma Farré, Luis M. Molinos-Albert, Abbey Evans, Anna Canela-Xandri, Richard M. Twyman, Jorge Carrillo, Raziel A. Ordóñez, Robin J. Shattock, Barry R. O’Keefe, Bonaventura Clotet, Julian Blanco, Gurdev S. Khush, Paul Christou, Teresa Capell
Publicado en: Proceedings of the National Academy of Sciences, Edición 115/33, 2018, Página(s) E7854-E7862, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1806022115

Optimizing product quality in molecular farming

Autores: Andreas Schiermeyer
Publicado en: Current Opinion in Biotechnology, Edición 61, 2020, Página(s) 15-20, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2019.08.012

Engineering the interactions between a plant‐produced HIV antibody and human Fc receptors

Autores: Szymon Stelter, Mathew J. Paul, Audrey Y.‐H. Teh, Melanie Grandits, Friedrich Altmann, Jessica Vanier, Muriel Bardor, Alexandra Castilho, Rachel Louise Allen, Julian K‐C. Ma
Publicado en: Plant Biotechnology Journal, Edición 18/2, 2019, Página(s) 402-414, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13207

Characterisation of a highly potent and near pan-neutralising anti-HIV monoclonal antibody expressed in tobacco plants

Autores: Catherine M. Moore, Melanie Grandits, Clemens Grünwald-Gruber, Friedrich Altmann, Maria Kotouckova, Audrey Y.-H. Teh & Julian K.-C. Ma
Publicado en: Retrovirology, Edición 18, 2021, ISSN 1742-4690
Editor: BioMed Central
DOI: 10.21203/rs.3.rs-104508/v1

Molecular Pharming for low and middle income countries

Autores: Sheeba Murad, Sebastian Fuller, Jonathan Menary, Cathy Moore, Elizabeth Pinneh, Tim Szeto, Inga Hitzeroth, Marcos Freire, Suthira Taychakhoonavudh, Waranyoo Phoolcharoen, Julian K-C Ma
Publicado en: Current Opinion in Biotechnology, Edición 61, 2020, Página(s) 53-59, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2019.10.005

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain.

Autores: Galas, Ludovic; Burel, Carole; Schapman, Damien; Ropitaux, Marc; Bernard, Sophie; Bénard, Magalie; Bardor, Muriel
Publicado en: Frontiers in Plant Science, Edición 2, 2021, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2021.638181

Contributions of the international plant science community to the fight against infectious diseases in humans-part 2: Affordable drugs in edible plants for endemic and re-emerging diseases.

Autores: He, Wenshu; Baysal, Can; Lobato, Maria; Huang, Xin; Alvarez, Derry; Zhu, Changfu; Armario-Nájera, Victoria; Blanco Perera, Amaya; Cerda, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes F.; Cunha, Nicolau B. da; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyapp
Publicado en: Plant Biotechnology Journal, 2021, Página(s) 1921-1936, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13658

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

Autores: Stefan Schillberg, Ricarda Finnern
Publicado en: Journal of Plant Physiology, Edición 258-259, 2021, Página(s) 153359, ISSN 0176-1617
Editor: Elsevier BV
DOI: 10.1016/j.jplph.2020.153359

Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay

Autores: Jan Pietschmann1*†, Nadja Voepel1†, Leonie Voß1, Stefan Rasche1, Max Schubert1, Michael Kleines2, Hans-Joachim Krause3, Tamlyn M. Shaw4, Holger Spiegel1‡ and Florian Schroeper1‡
Publicado en: Frontiers in Microbiology, 2021, ISSN 1664-302x
Editor: Frontiers Media
DOI: 10.3389/fmicb.2021.643275

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

Autores: Stefan Schillberg, Nicole Raven, Holger Spiegel, Stefan Rasche, Matthias Buntru
Publicado en: Frontiers in Plant Science, Edición 10, 2019, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.00720

Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression

Autores: Holger Spiegel, Stefan Schillberg & Greta Nölke
Publicado en: Methods in Molecular Biology - Recombinant Proteins in Plants, Edición 10643745, 2022, Página(s) 89-102, ISSN 1064-3745
Editor: Humana Press, Inc.
DOI: 10.1007/978-1-0716-2241-4_6

Conference proceedings (2)

Project proximities: A meta review of how design addresses distance in complex collaborations

Autores: Rosie Hornbuckle
Publicado en: Lockton, D., Lenzi, S., Hekkert, P., Oak, A., Sádaba, J., Lloyd, P. (eds.), DRS2022: Bilbao, 25 June - 3 July, Bilbao, Spain, 2022
Editor: Design Research Society
DOI: 10.21606/drs.2022.677

Beyond Science Communication: a service design approach to building mutual stakeholder understanding in the development of novel biotechnologies.

Autores: Hornbuckle, Rosie and Grimaldi, Silvia and Prendiville, Alison
Publicado en: Proceedings of the 6th conference on Design4Health. 2020 Lab4Living, Sheffield Hallam University, 2020, Página(s) 230-238, ISBN 978-1-8381117-0-0
Editor: Sheffield Hallam University
DOI: 10.5281/zenodo.7188867

Non-peer reviewed articles (1)

Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoors farms into biomanufacturing facilities

Autores: Diego-Martin, Borja; González Beatriz; Vázquez Vilar, Marta; Selma, Sara; Mateos-Fernández, Rubén; Gianoglio, Silvia; Fernández-del-Carmen, Asun; Orzáez, Diego
Publicado en: BioRxiv, 2020, ISSN 2692-8205
Editor: cold spring harbor laboratory
DOI: 10.1101/2020.10.13.331306

Derechos de propiedad intelectual

Utility model (1)

Product composed of a large-scale bioreactor specifically developed for hairy root growth

Número de solicitud/publicación: FR 22 305 436.2
Fecha: 2022-04-11
Solicitante(s): SAMABRIVA

Patent (2)

METHODS FOR THE IMPROVED RECOVERY OF SECRETED COMPOUNDS IN A HAIRY ROOTS-BASED EXPRESSION SYSTEM

Número de solicitud/publicación: 20 21083070
Fecha: 2021-11-25
Solicitante(s): SAMABRIVA

METHOD FOR ESTIMATING THE BIOMASS OF HAIRY ROOTS IN A MULTI-PHASIC CULTURE

Número de solicitud/publicación: 20 22067056
Fecha: 2022-06-22
Solicitante(s): SAMABRIVA