In proGIreg, four front-runner cities (FRC) (Dortmund DE; Turin IT; Zagreb HR; Ningbo CN) have created Living Labs (LLs). These areas suffer from social and economic disadvantages, inequality and other problems related to the decline of industries. They lack quality greenspaces, have a negative impact on human health and wellbeing and are more vulnerable to the effects of climate change. The LLs have implemented Nature-Based Solutions (NBS) which are citizen-owned and co-developed by state, market and civil society stakeholders. Innovation took place on the technical level through the NBS deployments, on the social level through co-designing, co-creating and co-implementing NBS and on the economic level through developing new NBS business models (BMs). Four follower cities (Cascais PT, Cluj-Napoca RO, Piraeus GR, Zenica BA) have tested the replicability of the NBS in new contexts. Each Follower City (FC) has organised a co-design process which has led to urban plans for NBS. The NBS that are tested i.a. include: regenerating soils with biotic compounds, creating community-based urban agriculture and aquaponics and making green corridors accessible. Scientific benefit assessment and monitoring results have been made available via numerous channels, including the EU NBS platform OPPLA, and contribute to the European reference framework for NBS, e.g. through the NBS monitoring guidelines and an interactive Business Model Catalogue (iBMC). Global impact has been achieved by the MOOC “Nature-based urban regeneration” distributed via edX and attended by learners from more than 100 countries. Internal and external replication events have been organized in all FRC to create and extend a community of practice in NBS.