Research empowerment on solute carriers (ReSOLUTE)

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Assays for SLCs based on fluorescent ligands, fluorescence-labelling of SLCs or fluorogenic probes

A report summarizing results from fluorescence-based assays.

Constructs and protocols for priority SLCs

Set of expression vectors and protein purification protocols for SLCs on the priority list.

Transcriptome and metabolome for priority SLCs

Quantitative differential transcriptome, metabolome data within a cell line that naturally expresses each SLC in the priority list in both basal and stimuli challenged states.

Data Management Plan 2

Initial version of DMP after 6 months, an updated version latest mid-term and a final version

Vector generation

Collection of lentiviral and Flp-In compatible vectors carrying CRISPR-based Cas9 and sgRNAs targeting all SLCs, together with vectors carrying tagged and untagged cDNAs for >80% of the SLC family

ReSOLUTE knowledgebase

Concept for database of integrated publicly available knowledge and ReSOLUTE web portal.

Data Mining

Data mining support for priority ranking of SLC family members for target selection in WP4.

Informatic anylysis of metabolomic data

Development and deployment of workflows for QSAR analysis of the ability of particular overexpressed SLCs to take up different substrates, and judicious variation of those substrates.

SLC overexpression in HEK293

Collection of >300 HEK293 isogenic cell lines, each overexpressing a SLC gene, quality-controlled and characterized with a multi-parameter approach

DEP implemented (stepwise, up to M60)
Subcellular localization of target SLCs

Based on optical imaging experiments with tagged SLCs.

SLC overexpressing cell lines

Collection of 600 cell lines each overexpressing a specific SLC in untagged and tagged versions qualitycontrolled and characterized with a multiparameter approach

SLC interactome

Physical measurement (by proteomics) of coexpression/copurification of SLCs with partners.

Signature metabolic profiles using targeted metabolomics

Robust identification and quantification using LCGCMS for some 180 central metabolites

SLC Interactome for priority SLCs

Condition dependent protein interaction network data in response to on-target and orthogonal pathway stimuli modulating SLC function.

Profile of plasma metabolite changes for each SLC cell line

Use of methods of Task 2.2 to assess the largest changes between cells lines upregulated/knocked out for particular SLCs

Assays for SLCs based on SSM-electrophysiology

A report summarizing results and protocols obtained with the SURFE2R approach.

Production of validated SLC antibodies

Large set of validated antibodies targeting SLCs, together with tissue localization data.

Assays for SLCs based on fluorescent sensor proteins

A report summarizing results and protocols on measuring SLC-dependent metabolic states through fluorescent sensor proteins.

Production of antigens and high-affinity binders

Antigens for animal immunization for priority SLCs, together with DARPins or monobodies for priority SLCS and a panel of relevant SLCs.

SLC k.o. cell lines

Collection of 600 cell lines each carrying frameshift mutations in one SLC gene qualitycontrolled and characterized with a multiparameter approach

Assays for SLCs based on cell lines that couple growth to SLC function

A report summarizing results and protocols on assay based on genetic engineering of cell lines dependent on specific SLCs for survival.

Assays for SLCs based on mass spectrometry, thermal shift, radiolabelled or fluorescent compounds

A report summarizing results from classical transport assays.

Assays for (electrogenic) transporters

A report summarizing results and protocols obtained using fluorescent sensitive dyes and impedance measurements.

Physiological and physical network map for priority SLCs

Condition-dependent regulatory models integrating transcriptomics, metabolomics and proteomics data for priority SLCs.

Data Management Plan 3

Initial version of DMP after 6 months, an updated version latest mid-term and a final version

Methodology for general detection of plasma metabolites and drug/dietary components

LC/GC-MS coupled to novel deconvolution routines for determining the approximately 3,000 molecules typically observable in serum

Protein expression screen

A family-wide assessment of protein expression yields in recombinant expression systems.

Determination of SLCs using ions as (co-)substrates

The recognition that many substrates are symporters or antiporters means that one can detect this by assessing the movement of co-ions when transport substrates are added to suitable cell lines.

SLC genetic interaction map

Production of ‘synthetic phenotypes’, based on the principle that knocking out both variants of a transporter with redundant function (e.g. loss of ability to transport X) thereby causes a phenotype from which one can infer function.

SLC k.o. in HEK293

Collection of 300 HEK293 isogenic cell lines each carrying frameshift mutations in a SLC gene qualitycontrolled and characterized with a multiparameter approach

Reconstitution of SLCs in proteoliposomes and nanodiscs

Nanodisc- and liposome-reconstituted SLCs for in vitro assays of successfully purified SLCs of the priority list.

Data Management Plan 1

Initial version of DMP after 6 months, an updated version latest mid-term and a final version.

Protein production for the SLCs in the priority list

Production of milligram quantities of highly pure (>90%) and stable protein samples for >30% of the priority SLCs.

ReSOLUTE web portal

Data sharing and approval process, and prototype of interface for data access at public web portal up and running

ReSOLUTE database

Infrastructure and data warehouse for sharing annotated raw data files and analysis results; prototype ready after first year and annual releases of new versions.

Publications

Immunofluorescence for protein binder validation

Auteurs: Gelová, Zuzana; Ingles-Prieto, Alvaro
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462234

Data Management Plan 2

Auteurs: Ulrich Goldmann, Gerhard Ecker, Vitaly Sedlyarov, Lia Scarabottolo, Vania Manolova, Claire Colas
Publié dans: 2020
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179816

Assays for (electrogenic) transporters

Auteurs: Lia Scarabottolo; Juergen Reinhardt; Huub Sijben
Publié dans: 2020
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179859

ReSOLUTE knowledgebase

Auteurs: Ulrich Goldmann; Vitaly Sedlyarov; Daniela Digles; Barbara Füzi
Publié dans: 2018
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179834

RESOLUTE: Data Management Plan

Auteurs: Goldmann, Ulrich; Ecker, Gerhard; Sedlyarov, Vitaly; Scarabottolo, Lia; Manolova, Vania; Colas, Claire
Publié dans: Numéro 1, 2019
Éditeur: n/a
DOI: 10.5281/zenodo.4309586

SLC k.o. cell lines

Auteurs: Gernot Wolf; Klaus Seuwen; Barbara Barbosa; Eirini Christodoulaki; Alvaro Ingles-Prieto; Ulrich Goldmann; Christoph Klimek; Svenja Onstein; Vitaly Sedlyarov; Tabea Wiedmer; Diana Santacruz; Coralie Viollet; Florence Massey; Veeranagouda Yaligara
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.10419148

RNA-Seq Transcriptomic Profiling

Auteurs: Viollet, Coralie; Santacruz, Diana; Knebel, Dagmar; Rust, Werner; Acker, Susanne; Dick, Alec
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462292

General protocol for SLC protein purification

Auteurs: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462714

Western Blotting of WT-OE cell lines

Auteurs: Svenja Onstein; Eva Liñeiro; Christoph Klimek; Barbara Barbosa; Gernot Wolf
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7457377

Antibody Characterization Report for Reduced folate transporter SLC19A1 (FOLT)

Auteurs: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733107

Ligation Independent Cloning of transporter gene into BacMam vector

Auteurs: Sauer, David B.; Huanyu Li; Raturi, Sagar; Scacioc, Andreea; Bohstedt, Tina; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7462743

Data mining

Auteurs: Daniela Digles; Jiani Li; Ulrich Goldmann
Publié dans: 2018
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179841

High-throughput immunofluorescence to validate high-affinity binders

Auteurs: Alvaro Ingles-Prieto; Felix Kartnig
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7457392

Mitochondrial enrichment followed by Affinity Purification couple to Mass Spectrometry (AP-MS)

Auteurs: Eva Liñeiro; Alvaro Ingles-Prieto; Fabian Frommelt; André C. Mueller
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462207

SLC overexpression in HEK293

Auteurs: Wolf, Gernot; Seuwen, Klaus
Publié dans: 2021
Éditeur: n/a
DOI: 10.5281/zenodo.5566804

Large Scale Transduction of Expi293F Cells with BacMam Virus

Auteurs: Sauer, David B.; Huanyu Li; Raturi, Sagar; Scacioc, Andreea; Bohstedt, Tina; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462911

Expression analysis and co-localization imaging of Jump In T-REx HEK293 WT-OE cell pools

Auteurs: Pfeifer, Martin; Kohlbrenner, Mariah; Chang, Lena; Lambrigger, Pascal; Ingles-Prieto, Alvaro; Reinhardt, Jürgen
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7457345

Generation of Jump-In T-REx HEK293 wild type-overexpression cell lines

Auteurs: Klimek, Christoph; Onstein, Svenja; Barbosa, Barbara; Ingles-Prieto, Alvaro; Skucha, Anna; Ibig, Yvonne; Seuwen, Klaus; Wolf, Gernot
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7457220

Construction of a Bacmid (BacMam) vector containing the SLC gene

Auteurs: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462780

Antibody Characterization Report for Equilibrative nucleoside transporter 1 SLC29A1 (ENT1)

Auteurs: Alshafie, Walaa; Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733134

Transfection to Generate BacMam Virus containing SLC transporter and small scale expression testing

Auteurs: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462876

Vector generation

Auteurs: Gernot Wolf; Alvaro Ingles-Prieto; Enrico Girardi; Vitaly Sedlyarov; Svenja Onstein; Christoph Klimek; Barbara Barbosa
Publié dans: 2019
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179824

Assays for SLCs based on fluorescent sensor proteins

Auteurs: Philipp Leippe; Lia Scarabottolo
Publié dans: 2020
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179857

Antibody Characterization Report for Very long-chain acyl-CoA synthetase SLC27A2 (VLCS)

Auteurs: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733132

RNA extraction and purification

Auteurs: Klimek, Christoph; Onstein, Svenja; Barbosa, Barbara; Wolf, Gernot
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462188

SLC overexpressing cell lines

Auteurs: Gernot Wolf; Klaus Seuwen; Barbara Barbosa; Eirini Christodoulaki; Alvaro Ingles-Prieto; Ulrich Goldmann; Christoph Klimek; Svenja Onstein; Vitaly Sedlyarov; Tabea Wiedmer; Diana Santacruz; Coralie Viollet
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.10419229

Targeted Metabolomics Sample Preparation

Auteurs: Tabea Wiedmer; Abigail Jarret; Sabrina Lindinger
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462174

Antibody Characterization Report for Solute carrier family 2, facilitated glucose transporter member 6 SLC2A6 (GLUT-6)

Auteurs: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733123

Generation of Single Cell Knockout Clones

Auteurs: Svenja Onstein; Christoph Klimek; Barbara Barbosa; Anna Skucha; Gernot Wolf
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7457297

Affinity Purification couple to Mass Spectrometry (AP-MS)

Auteurs: Alvaro Ingles-Prieto; Eva Liñeiro; Ariel Bensimon; Fabian Frommelt; André C. Mueller
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7457416

Proteomics characterization of RESOLUTE parental cell lines

Auteurs: Eva Liñeiro; André C. Mueller
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7462271

Methodology for general detection of plasma metabolites and drug/dietary components

Auteurs: Claire Steppan; Marina Wright Muelas; Douglas Kell; Tabea Wiedmer; Mary Piotrowski
Publié dans: 2019
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179870

Purification of SPNS2 from Expi293FTM Cells

Auteurs: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7468558

ReSOLUTE web portal

Auteurs: Ulrich Goldmann; Vitaly Sedlyarov
Publié dans: 2019
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179880

Antibody Characterization Report for Excitatory amino acid transporter 1 SLC1A3 (EAAT1)

Auteurs: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733103

Spatial proteomics couple to Mass Spectrometry (BioID-MS)

Auteurs: Ingles-Prieto, Alvaro; Liñeiro, Eva; Frommelt, Fabian
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7457436

ReSOLUTE database

Auteurs: Ulrich Goldmann; Vitaly Sedlyarov
Publié dans: 2019
Éditeur: Zenodo
DOI: 10.5281/zenodo.5179889

Signature metabolic profiles using targeted metabolomics

Auteurs: Tabea Wiedmer, Eirini Christodoulaki, Sabrina Lindinger, Iciar Serrano, Christoph Bueschl, Thomas Hannich
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.10419241

SLC k.o. in HEK293

Mass Spectrometry analysis (data acquisition)

Auteurs: Mueller, André C.; Liñeiro, Eva
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7462252

SLC4A4 antibody characterization report

Auteurs: Garcia, Julio; Gelova, Zuzana
Publié dans: 2023
Éditeur: n/a
DOI: 10.5281/zenodo.10033541

Protein production of SLC63A2

Auteurs: Vera Pütter; Yung-Ning Chang
Publié dans: 2022
Éditeur: Zenodo
DOI: 10.5281/zenodo.7468532

Generation of Knockout-Overexpression Cell Lines

Auteurs: Christoph Klimek; Svenja Onstein; Barbara Barbosa; Alvaro Ingles-Prieto; Anna Skucha; Gernot Wolf
Publié dans: 2022
Éditeur: n/a
DOI: 10.5281/zenodo.7457295

Antibody Characterization Report for Glucose-6-phosphate exchanger SLC37A4 (G6PT)

Auteurs: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl
Publié dans: 2021
Éditeur: Zenodo
DOI: 10.5281/zenodo.4733121

Automation of high-throughput mRNA-seq library preparation: a robust, hands-free and time efficient methodology

Auteurs: Diana Santacruz, Francis O Enane, Katrin Fundel-Clemens, Martin Giner, Gernot Wolf, Svenja Onstein, Christoph Klimek, Zachary Smith, Bhagya Wijayawardena, Coralie Viollet
Publié dans: SLAS Discovery, 2022, ISSN 2472-5552
Éditeur: Elsevier
DOI: 10.1016/j.slasd.2022.01.002

The macrocycle inhibitor landscape of SLC-transporter

Auteurs: Granulo N, Sosnin S, Digles D, Ecker G.
Publié dans: Molecular Informatics, 2024, ISSN 1868-1743
Éditeur: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/minf.202300287

MPP+-Induced Changes in Cellular Impedance as a Measure for Organic Cation Transporter (SLC22A1-3) Activity and Inhibition

Auteurs: Mocking TAM, Sijben HJ, Vermeulen YW, IJzerman AP, Heitman LH
Publié dans: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23031203

Development of a live cell assay for the zinc transporter ZnT8.

Auteurs: Azzollini L, Prete DD, Wolf G, Klimek C, Saggioro M, Ricci F, Christodoulaki E, Wiedmer T, Ingles-Prieto A, Superti-Furga G, Scarabottolo L.
Publié dans: SLAS Discovery, 2024, ISSN 2472-5560
Éditeur: Elsevier Inc.
DOI: 10.1016/j.slasd.2024.100166

Experimental and Computational Analysis of Newly Identified Pathogenic Mutations in the Creatine Transporter SLC6A8

Auteurs: Evandro Ferrada, Tabea Wiedmer, Wen-An Wang, Fabian Frommelt, Barbara Steurer, Christoph Klimek, Sabrina Lindinger, Tanja Osthushenrich, Andrea Garofoli, Silvia Brocchetti, Samuel Bradberry, Jiahui Huang, Aidan MacNamara, Lia Scarabottolo, Gerhard F. Ecker, Anders Malarstig, Giulio Superti-Furga
Publié dans: Journal of Molecular Biology, 2024, ISSN 0022-2836
Éditeur: Academic Press
DOI: 10.1016/j.jmb.2023.168383

Functional characterization of SLC39 family members ZIP5 and ZIP10 in overexpressing HEK293 cells reveals selective copper transport activity

Auteurs: Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V.
Publié dans: Biometals, 2023, ISSN 0966-0844
Éditeur: Kluwer Academic Publishers
DOI: 10.1007/s10534-022-00474-6

High-throughput expression and purification of human solute carriers for structural and biochemical studies

Auteurs: Raturi S, Li H, Chang YN, Scacioc A, Bohstedt T, Fernandez-Cid A, Evans A, Abrusci P, Balakrishnan A, Pascoa TC, He D, Chi G, Kaur Singh N, Ye M, Li A, Shrestha L, Wang D, Williams EP, Burgess-Brown NA, Dürr KL, Puetter V, Ingles-Prieto A, Sauer DB.
Publié dans: Journal of Visualized Experiments, 2023, ISSN 1940-087X
Éditeur: MYJoVE Corporation
DOI: 10.3791/65878

An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

Auteurs: Vojtech Dvorak, Tabea Wiedmer, Alvaro Ingles-Prieto, Patrick Altermatt, Helena Batoulis, Felix Bärenz, Eckhard Bender, Daniela Digles, Franz Dürrenberger, Laura H. Heitman, Adriaan P. IJzerman, Douglas B. Kell, Stefanie Kickinger, Daniel Körzö, Philipp Leippe, Thomas Licher, Vania Manolova, Riccardo Rizzetto, Francesca Sassone, Lia Scarabottolo, Avner Schlessinger, Vanessa Schneider, Hubert J.
Publié dans: Frontiers in Pharmacology, Numéro 12, 2021, ISSN 1663-9812
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fphar.2021.722889

Structural and functional annotation of solute carrier transporters: implication for drug discovery

Auteurs: Vojtech Dvorak & Giulio Superti-Furga
Publié dans: Expert Opinion on Drug Discovery, 2023, ISSN 1746-0441
Éditeur: Informa Healthcare
DOI: 10.1080/17460441.2023.2244760

A substrate‐based ontology for human solute carriers

Auteurs: Eva Meixner, Ulrich Goldmann, Vitaly Sedlyarov, Stefania Scorzoni, Manuele Rebsamen, Enrico Girardi, Giulio Superti‐Furga
Publié dans: Molecular Systems Biology, Numéro 16/7, 2020, ISSN 1744-4292
Éditeur: Nature Publishing Group
DOI: 10.15252/msb.20209652

Label-free high-throughput screening assay for the identification of norepinephrine transporter (NET/SLC6A2) inhibitors

Auteurs: Hubert J. Sijben, Wieke M. van Oostveen, Peter B. R. Hartog, Laura Stucchi, Andrea Rossignoli, Giovanna Maresca, Lia Scarabottolo, Adriaan P. IJzerman, Laura H. Heitman
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-91700-7

Targeting solute carriers to modulate receptor–ligand interactions

Auteurs: Hubert J. Sijben, Giulio Superti-Furga, Adriaan P. IJzerman, Laura H. Heitman
Publié dans: Trends in Pharmacological Sciences, 2022, ISSN 0165-6147
Éditeur: Elsevier BV
DOI: 10.1016/j.tips.2022.02.004

Accelerating SLC Transporter Research: Streamlining Knowledge and Validated Tools

Auteurs: Tabea Wiedmer, Alvaro Ingles-Prieto, Ulrich Goldmann, Claire M. Steppan, Giulio Superti-Furga, the RESOLUTE, REsolution consortia
Publié dans: Clinical Pharmacology & Therapeutics, Numéro 00099236, 2022, ISSN 0009-9236
Éditeur: Nature Publishing Group
DOI: 10.1002/cpt.2639

Image-based quantification of mitochondrial iron uptake via Mitoferrin-2

Auteurs: Polesel M, Wildschut MHE, Doucerain C, Kuhn M, Flace A, Steck AL, Wilhelm M, Ingles-Prieto A, Wiedmer T, Superti-Furga G, Manolova V, Dürrenberger F.
Publié dans: Mitochondrion, 2024, ISSN 1567-7249
Éditeur: Elsevier BV
DOI: 10.1016/j.mito.2024.101889

Solid-supported membrane (SSM)-based electrophysiology assays using surface electrogenic event reader technology (SURFE²R) in early drug discovery

Auteurs: Pommereau, A., Licher, T., & Bärenz, F.
Publié dans: Current Protocols, 2023, ISSN 2691-1299
Éditeur: Wiley
DOI: 10.1002/cpz1.650

Label-free detection of prostaglandin transporter (SLCO2A1) function and inhibition: insights by wound healing and TRACT assays

Auteurs: Mocking TAM, van Oostveen W, Van Veldhoven JP, Minnee H, Fehres C, Whitehurst C, IJzerman AP, Heitman LH.
Publié dans: Front. Pharmacol., 2024, ISSN 1663-9812
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fphar.2024.1372109

Proteochemometric Modeling Identifies Chemically Diverse Norepinephrine Transporter Inhibitors

Auteurs: Brandon J. Bongers, Huub J. Sijben, Peter B. R. Hartog, Andrey Tarnovskiy, Adriaan P. IJzerman, Laura H. Heitman, and Gerard J. P. van Westen
Publié dans: Journal of Chemical Information and Modeling, 2023, ISSN 1549-9596
Éditeur: American Chemical Society
DOI: 10.1021/acs.jcim.2c01645

Studies of structural determinants of substrate binding in the Creatine Transporter (CreaT, SLC6A8) using molecular models

Auteurs: Claire Colas, Giulia Banci, Riccardo Martini, Gerhard F. Ecker
Publié dans: Scientific Reports, Numéro 10/1, 2020, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-020-63189-z

Toward a Systematic Structural and Functional Annotation of Solute Carriers Transporters-Example of the SLC6 and SLC7 Families.

Auteurs: Claire Colas
Publié dans: Frontiers in Pharmacology, 2020, ISSN 1663-9812
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fphar.2020.01229

Impedance-Based Phenotypic Readout of Transporter Function: A Case for Glutamate Transporters

Auteurs: Sijben HJ, Dall’ Acqua L, Liu R, Jarret A, Christodoulaki E, Onstein S, Wolf G, Verburgt SJ, Le Dévédec SE, Wiedmer T, Superti-Furga G, IJzerman AP and Heitman LH
Publié dans: Frontiers in Pharmacology, 2022, ISSN 1663-9812
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fphar.2022.872335

A study of the dopamine transporter using the TRACT assay, a novel in vitro tool for solute carrier drug discovery

Auteurs: Hubert J. Sijben, Julie J. E. van den Berg, Jeremy D. Broekhuis, Adriaan P. IJzerman, Laura H. Heitman
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-020-79218-w

Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions

Auteurs: Manuele Rebsamen, Enrico Girardi, Vitaly Sedlyarov, Stefania Scorzoni, Konstantinos Papakostas, Manuela Vollert, Justyna Konecka, Bettina Guertl, Kristaps Klavins, Tabea Wiedmer, Giulio Superti-Furga
Publié dans: Life Science Alliance, Numéro 25751077, 2022, ISSN 2575-1077
Éditeur: Life Science Alliance, LLC
DOI: 10.26508/lsa.202201404

Still in Search for an EAAT Activator: GT949 Does Not Activate EAAT2, nor EAAT3 in Impedance and Radioligand Uptake Assays

Auteurs: Lieve van Veggel, Tamara A.M. Mocking, Hubert J. Sijben, Rongfang Liu, Marina Gorostiola González, Majlen A. Dilweg, Jeroen Royakkers, Anna Li, Vijay Kumar, Yin Yao Dong, Alex Bullock, David B. Sauer, Hanne Diliën, Gerard J.P. van Westen, Rudy Schreiber, Laura H. Heitman, and Tim Vanmierlo
Publié dans: ACS Chemical Neuroscience, 2024, ISSN 1948-7193
Éditeur: American Chemical Society
DOI: 10.1021/acschemneuro.3c00731

The RESOLUTE consortium: unlocking SLC transporters for drug discovery

Auteurs: Giulio Superti-Furga, Daniel Lackner, Tabea Wiedmer, Alvaro Ingles-Prieto, Barbara Barbosa, Enrico Girardi, Ulrich Goldmann, Bettina Gürtl, Kristaps Klavins, Christoph Klimek, Sabrina Lindinger, Eva Liñeiro-Retes, André C. Müller, Svenja Onstein, Gregor Redinger, Daniela Reil, Vitaly Sedlyarov, Gernot Wolf, Matthew Crawford, Robert Everley, David Hepworth, Shenping Liu, Stephen Noell, Mary Pio
Publié dans: Nature Reviews Drug Discovery, Numéro 19/7, 2020, Page(s) 429-430, ISSN 1474-1776
Éditeur: Nature Publishing Group
DOI: 10.1038/d41573-020-00056-6

Recherche de données OpenAIRE...

Livrables

Publications

Partager cette page Partager cette page sur les réseaux sociaux

Télécharger Télécharger le contenu de la page