Resultado final Documents, reports (13) Data Management Plan 2 Initial version of DMP after 6 months, an updated version latest mid-term and a final version Vector generation Collection of lentiviral and Flp-In compatible vectors carrying CRISPR-based Cas9 and sgRNAs targeting all SLCs, together with vectors carrying tagged and untagged cDNAs for >80% of the SLC family ReSOLUTE knowledgebase Concept for database of integrated publicly available knowledge and ReSOLUTE web portal. Data Mining Data mining support for priority ranking of SLC family members for target selection in WP4. SLC overexpression in HEK293 Collection of >300 HEK293 isogenic cell lines, each overexpressing a SLC gene, quality-controlled and characterized with a multi-parameter approach SLC overexpressing cell lines Collection of 600 cell lines each overexpressing a specific SLC in untagged and tagged versions qualitycontrolled and characterized with a multiparameter approach Signature metabolic profiles using targeted metabolomics Robust identification and quantification using LCGCMS for some 180 central metabolites Assays for SLCs based on fluorescent sensor proteins A report summarizing results and protocols on measuring SLC-dependent metabolic states through fluorescent sensor proteins. SLC k.o. cell lines Collection of 600 cell lines each carrying frameshift mutations in one SLC gene qualitycontrolled and characterized with a multiparameter approach Assays for (electrogenic) transporters A report summarizing results and protocols obtained using fluorescent sensitive dyes and impedance measurements. Methodology for general detection of plasma metabolites and drug/dietary components LC/GC-MS coupled to novel deconvolution routines for determining the approximately 3,000 molecules typically observable in serum SLC k.o. in HEK293 Collection of 300 HEK293 isogenic cell lines each carrying frameshift mutations in a SLC gene qualitycontrolled and characterized with a multiparameter approach Data Management Plan 1 Initial version of DMP after 6 months, an updated version latest mid-term and a final version. Websites, patent fillings, videos etc. (2) ReSOLUTE web portal Data sharing and approval process, and prototype of interface for data access at public web portal up and running ReSOLUTE database Infrastructure and data warehouse for sharing annotated raw data files and analysis results; prototype ready after first year and annual releases of new versions. Publicaciones Other (44) Immunofluorescence for protein binder validation Autores: Gelová, Zuzana; Ingles-Prieto, Alvaro Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462234 Data Management Plan 2 Autores: Ulrich Goldmann, Gerhard Ecker, Vitaly Sedlyarov, Lia Scarabottolo, Vania Manolova, Claire Colas Publicado en: 2020 Editor: Zenodo DOI: 10.5281/zenodo.5179816 Assays for (electrogenic) transporters Autores: Lia Scarabottolo; Juergen Reinhardt; Huub Sijben Publicado en: 2020 Editor: Zenodo DOI: 10.5281/zenodo.5179859 ReSOLUTE knowledgebase Autores: Ulrich Goldmann; Vitaly Sedlyarov; Daniela Digles; Barbara Füzi Publicado en: 2018 Editor: Zenodo DOI: 10.5281/zenodo.5179834 RESOLUTE: Data Management Plan Autores: Goldmann, Ulrich; Ecker, Gerhard; Sedlyarov, Vitaly; Scarabottolo, Lia; Manolova, Vania; Colas, Claire Publicado en: Issue 1, 2019 Editor: n/a DOI: 10.5281/zenodo.4309586 SLC k.o. cell lines Autores: Gernot Wolf; Klaus Seuwen; Barbara Barbosa; Eirini Christodoulaki; Alvaro Ingles-Prieto; Ulrich Goldmann; Christoph Klimek; Svenja Onstein; Vitaly Sedlyarov; Tabea Wiedmer; Diana Santacruz; Coralie Viollet; Florence Massey; Veeranagouda Yaligara Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.10419148 RNA-Seq Transcriptomic Profiling Autores: Viollet, Coralie; Santacruz, Diana; Knebel, Dagmar; Rust, Werner; Acker, Susanne; Dick, Alec Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462292 General protocol for SLC protein purification Autores: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462714 Western Blotting of WT-OE cell lines Autores: Svenja Onstein; Eva Liñeiro; Christoph Klimek; Barbara Barbosa; Gernot Wolf Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7457377 Antibody Characterization Report for Reduced folate transporter SLC19A1 (FOLT) Autores: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733107 Ligation Independent Cloning of transporter gene into BacMam vector Autores: Sauer, David B.; Huanyu Li; Raturi, Sagar; Scacioc, Andreea; Bohstedt, Tina; Abilasha Balakrishnan Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7462743 Data mining Autores: Daniela Digles; Jiani Li; Ulrich Goldmann Publicado en: 2018 Editor: Zenodo DOI: 10.5281/zenodo.5179841 High-throughput immunofluorescence to validate high-affinity binders Autores: Alvaro Ingles-Prieto; Felix Kartnig Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7457392 Mitochondrial enrichment followed by Affinity Purification couple to Mass Spectrometry (AP-MS) Autores: Eva Liñeiro; Alvaro Ingles-Prieto; Fabian Frommelt; André C. Mueller Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462207 SLC overexpression in HEK293 Autores: Wolf, Gernot; Seuwen, Klaus Publicado en: 2021 Editor: n/a DOI: 10.5281/zenodo.5566804 Large Scale Transduction of Expi293F Cells with BacMam Virus Autores: Sauer, David B.; Huanyu Li; Raturi, Sagar; Scacioc, Andreea; Bohstedt, Tina; Abilasha Balakrishnan Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462911 Expression analysis and co-localization imaging of Jump In T-REx HEK293 WT-OE cell pools Autores: Pfeifer, Martin; Kohlbrenner, Mariah; Chang, Lena; Lambrigger, Pascal; Ingles-Prieto, Alvaro; Reinhardt, Jürgen Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7457345 Generation of Jump-In T-REx HEK293 wild type-overexpression cell lines Autores: Klimek, Christoph; Onstein, Svenja; Barbosa, Barbara; Ingles-Prieto, Alvaro; Skucha, Anna; Ibig, Yvonne; Seuwen, Klaus; Wolf, Gernot Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7457220 Construction of a Bacmid (BacMam) vector containing the SLC gene Autores: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462780 Antibody Characterization Report for Equilibrative nucleoside transporter 1 SLC29A1 (ENT1) Autores: Alshafie, Walaa; Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733134 Transfection to Generate BacMam Virus containing SLC transporter and small scale expression testing Autores: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462876 Vector generation Autores: Gernot Wolf; Alvaro Ingles-Prieto; Enrico Girardi; Vitaly Sedlyarov; Svenja Onstein; Christoph Klimek; Barbara Barbosa Publicado en: 2019 Editor: Zenodo DOI: 10.5281/zenodo.5179824 Assays for SLCs based on fluorescent sensor proteins Autores: Philipp Leippe; Lia Scarabottolo Publicado en: 2020 Editor: Zenodo DOI: 10.5281/zenodo.5179857 Antibody Characterization Report for Very long-chain acyl-CoA synthetase SLC27A2 (VLCS) Autores: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733132 RNA extraction and purification Autores: Klimek, Christoph; Onstein, Svenja; Barbosa, Barbara; Wolf, Gernot Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462188 SLC overexpressing cell lines Autores: Gernot Wolf; Klaus Seuwen; Barbara Barbosa; Eirini Christodoulaki; Alvaro Ingles-Prieto; Ulrich Goldmann; Christoph Klimek; Svenja Onstein; Vitaly Sedlyarov; Tabea Wiedmer; Diana Santacruz; Coralie Viollet Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.10419229 Targeted Metabolomics Sample Preparation Autores: Tabea Wiedmer; Abigail Jarret; Sabrina Lindinger Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462174 Antibody Characterization Report for Solute carrier family 2, facilitated glucose transporter member 6 SLC2A6 (GLUT-6) Autores: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733123 Generation of Single Cell Knockout Clones Autores: Svenja Onstein; Christoph Klimek; Barbara Barbosa; Anna Skucha; Gernot Wolf Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7457297 Affinity Purification couple to Mass Spectrometry (AP-MS) Autores: Alvaro Ingles-Prieto; Eva Liñeiro; Ariel Bensimon; Fabian Frommelt; André C. Mueller Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7457416 Proteomics characterization of RESOLUTE parental cell lines Autores: Eva Liñeiro; André C. Mueller Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7462271 Methodology for general detection of plasma metabolites and drug/dietary components Autores: Claire Steppan; Marina Wright Muelas; Douglas Kell; Tabea Wiedmer; Mary Piotrowski Publicado en: 2019 Editor: Zenodo DOI: 10.5281/zenodo.5179870 Purification of SPNS2 from Expi293FTM Cells Autores: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7468558 ReSOLUTE web portal Autores: Ulrich Goldmann; Vitaly Sedlyarov Publicado en: 2019 Editor: Zenodo DOI: 10.5281/zenodo.5179880 Antibody Characterization Report for Excitatory amino acid transporter 1 SLC1A3 (EAAT1) Autores: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733103 Spatial proteomics couple to Mass Spectrometry (BioID-MS) Autores: Ingles-Prieto, Alvaro; Liñeiro, Eva; Frommelt, Fabian Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7457436 ReSOLUTE database Autores: Ulrich Goldmann; Vitaly Sedlyarov Publicado en: 2019 Editor: Zenodo DOI: 10.5281/zenodo.5179889 Signature metabolic profiles using targeted metabolomics Autores: Tabea Wiedmer, Eirini Christodoulaki, Sabrina Lindinger, Iciar Serrano, Christoph Bueschl, Thomas Hannich Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.10419241 SLC k.o. in HEK293 Autores: Gernot Wolf; Klaus Seuwen; Barbara Barbosa; Eirini Christodoulaki; Alvaro Ingles-Prieto; Ulrich Goldmann; Christoph Klimek; Svenja Onstein; Vitaly Sedlyarov; Tabea Wiedmer; Diana Santacruz; Coralie Viollet; Florence Massey; Veeranagouda Yaligara Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.10419196 Mass Spectrometry analysis (data acquisition) Autores: Mueller, André C.; Liñeiro, Eva Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7462252 SLC4A4 antibody characterization report Autores: Garcia, Julio; Gelova, Zuzana Publicado en: 2023 Editor: n/a DOI: 10.5281/zenodo.10033541 Protein production of SLC63A2 Autores: Vera Pütter; Yung-Ning Chang Publicado en: 2022 Editor: Zenodo DOI: 10.5281/zenodo.7468532 Generation of Knockout-Overexpression Cell Lines Autores: Christoph Klimek; Svenja Onstein; Barbara Barbosa; Alvaro Ingles-Prieto; Anna Skucha; Gernot Wolf Publicado en: 2022 Editor: n/a DOI: 10.5281/zenodo.7457295 Antibody Characterization Report for Glucose-6-phosphate exchanger SLC37A4 (G6PT) Autores: Ayoubi, Riham; McPherson, Peter S.; Laflamme, Carl Publicado en: 2021 Editor: Zenodo DOI: 10.5281/zenodo.4733121 Peer reviewed articles (19) Automation of high-throughput mRNA-seq library preparation: a robust, hands-free and time efficient methodology Autores: Diana Santacruz, Francis O Enane, Katrin Fundel-Clemens, Martin Giner, Gernot Wolf, Svenja Onstein, Christoph Klimek, Zachary Smith, Bhagya Wijayawardena, Coralie Viollet Publicado en: SLAS Discovery, 2022, ISSN 2472-5552 Editor: Elsevier DOI: 10.1016/j.slasd.2022.01.002 The macrocycle inhibitor landscape of SLC-transporter Autores: Granulo N, Sosnin S, Digles D, Ecker G. Publicado en: Molecular Informatics, 2024, ISSN 1868-1743 Editor: Wiley - VCH Verlag GmbH & CO. KGaA DOI: 10.1002/minf.202300287 MPP+-Induced Changes in Cellular Impedance as a Measure for Organic Cation Transporter (SLC22A1-3) Activity and Inhibition Autores: Mocking TAM, Sijben HJ, Vermeulen YW, IJzerman AP, Heitman LH Publicado en: International Journal of Molecular Sciences, 2022, ISSN 1422-0067 Editor: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/ijms23031203 Experimental and Computational Analysis of Newly Identified Pathogenic Mutations in the Creatine Transporter SLC6A8 Autores: Evandro Ferrada, Tabea Wiedmer, Wen-An Wang, Fabian Frommelt, Barbara Steurer, Christoph Klimek, Sabrina Lindinger, Tanja Osthushenrich, Andrea Garofoli, Silvia Brocchetti, Samuel Bradberry, Jiahui Huang, Aidan MacNamara, Lia Scarabottolo, Gerhard F. Ecker, Anders Malarstig, Giulio Superti-Furga Publicado en: Journal of Molecular Biology, 2024, ISSN 0022-2836 Editor: Academic Press DOI: 10.1016/j.jmb.2023.168383 Functional characterization of SLC39 family members ZIP5 and ZIP10 in overexpressing HEK293 cells reveals selective copper transport activity Autores: Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V. Publicado en: Biometals, 2023, ISSN 0966-0844 Editor: Kluwer Academic Publishers DOI: 10.1007/s10534-022-00474-6 High-throughput expression and purification of human solute carriers for structural and biochemical studies Autores: Raturi S, Li H, Chang YN, Scacioc A, Bohstedt T, Fernandez-Cid A, Evans A, Abrusci P, Balakrishnan A, Pascoa TC, He D, Chi G, Kaur Singh N, Ye M, Li A, Shrestha L, Wang D, Williams EP, Burgess-Brown NA, Dürr KL, Puetter V, Ingles-Prieto A, Sauer DB. Publicado en: Journal of Visualized Experiments, 2023, ISSN 1940-087X Editor: MYJoVE Corporation DOI: 10.3791/65878 An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters Autores: Vojtech Dvorak, Tabea Wiedmer, Alvaro Ingles-Prieto, Patrick Altermatt, Helena Batoulis, Felix Bärenz, Eckhard Bender, Daniela Digles, Franz Dürrenberger, Laura H. Heitman, Adriaan P. IJzerman, Douglas B. Kell, Stefanie Kickinger, Daniel Körzö, Philipp Leippe, Thomas Licher, Vania Manolova, Riccardo Rizzetto, Francesca Sassone, Lia Scarabottolo, Avner Schlessinger, Vanessa Schneider, Hubert J. Publicado en: Frontiers in Pharmacology, Issue 12, 2021, ISSN 1663-9812 Editor: Frontiers Media S.A. DOI: 10.3389/fphar.2021.722889 Structural and functional annotation of solute carrier transporters: implication for drug discovery Autores: Vojtech Dvorak & Giulio Superti-Furga Publicado en: Expert Opinion on Drug Discovery, 2023, ISSN 1746-0441 Editor: Informa Healthcare DOI: 10.1080/17460441.2023.2244760 A substrate‐based ontology for human solute carriers Autores: Eva Meixner, Ulrich Goldmann, Vitaly Sedlyarov, Stefania Scorzoni, Manuele Rebsamen, Enrico Girardi, Giulio Superti‐Furga Publicado en: Molecular Systems Biology, Issue 16/7, 2020, ISSN 1744-4292 Editor: Nature Publishing Group DOI: 10.15252/msb.20209652 Label-free high-throughput screening assay for the identification of norepinephrine transporter (NET/SLC6A2) inhibitors Autores: Hubert J. Sijben, Wieke M. van Oostveen, Peter B. R. Hartog, Laura Stucchi, Andrea Rossignoli, Giovanna Maresca, Lia Scarabottolo, Adriaan P. IJzerman, Laura H. Heitman Publicado en: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322 Editor: Nature Publishing Group DOI: 10.1038/s41598-021-91700-7 Targeting solute carriers to modulate receptor–ligand interactions Autores: Hubert J. Sijben, Giulio Superti-Furga, Adriaan P. IJzerman, Laura H. Heitman Publicado en: Trends in Pharmacological Sciences, 2022, ISSN 0165-6147 Editor: Elsevier BV DOI: 10.1016/j.tips.2022.02.004 Accelerating SLC Transporter Research: Streamlining Knowledge and Validated Tools Autores: Tabea Wiedmer, Alvaro Ingles-Prieto, Ulrich Goldmann, Claire M. Steppan, Giulio Superti-Furga, the RESOLUTE, REsolution consortia Publicado en: Clinical Pharmacology & Therapeutics, Issue 00099236, 2022, ISSN 0009-9236 Editor: Nature Publishing Group DOI: 10.1002/cpt.2639 Solid-supported membrane (SSM)-based electrophysiology assays using surface electrogenic event reader technology (SURFE²R) in early drug discovery Autores: Pommereau, A., Licher, T., & Bärenz, F. Publicado en: Current Protocols, 2023, ISSN 2691-1299 Editor: Wiley DOI: 10.1002/cpz1.650 Proteochemometric Modeling Identifies Chemically Diverse Norepinephrine Transporter Inhibitors Autores: Brandon J. Bongers, Huub J. Sijben, Peter B. R. Hartog, Andrey Tarnovskiy, Adriaan P. IJzerman, Laura H. Heitman, and Gerard J. P. van Westen Publicado en: Journal of Chemical Information and Modeling, 2023, ISSN 1549-9596 Editor: American Chemical Society DOI: 10.1021/acs.jcim.2c01645 Studies of structural determinants of substrate binding in the Creatine Transporter (CreaT, SLC6A8) using molecular models Autores: Claire Colas, Giulia Banci, Riccardo Martini, Gerhard F. Ecker Publicado en: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322 Editor: Nature Publishing Group DOI: 10.1038/s41598-020-63189-z Toward a Systematic Structural and Functional Annotation of Solute Carriers Transporters-Example of the SLC6 and SLC7 Families. Autores: Claire Colas Publicado en: Frontiers in Pharmacology, 2020, ISSN 1663-9812 Editor: Frontiers Media S.A. DOI: 10.3389/fphar.2020.01229 Impedance-Based Phenotypic Readout of Transporter Function: A Case for Glutamate Transporters Autores: Sijben HJ, Dall’ Acqua L, Liu R, Jarret A, Christodoulaki E, Onstein S, Wolf G, Verburgt SJ, Le Dévédec SE, Wiedmer T, Superti-Furga G, IJzerman AP and Heitman LH Publicado en: Frontiers in Pharmacology, 2022, ISSN 1663-9812 Editor: Frontiers Media S.A. DOI: 10.3389/fphar.2022.872335 A study of the dopamine transporter using the TRACT assay, a novel in vitro tool for solute carrier drug discovery Autores: Hubert J. Sijben, Julie J. E. van den Berg, Jeremy D. Broekhuis, Adriaan P. IJzerman, Laura H. Heitman Publicado en: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322 Editor: Nature Publishing Group DOI: 10.1038/s41598-020-79218-w Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions Autores: Manuele Rebsamen, Enrico Girardi, Vitaly Sedlyarov, Stefania Scorzoni, Konstantinos Papakostas, Manuela Vollert, Justyna Konecka, Bettina Guertl, Kristaps Klavins, Tabea Wiedmer, Giulio Superti-Furga Publicado en: Life Science Alliance, Issue 25751077, 2022, ISSN 2575-1077 Editor: Life Science Alliance, LLC DOI: 10.26508/lsa.202201404 Non-peer reviewed articles (1) The RESOLUTE consortium: unlocking SLC transporters for drug discovery Autores: Giulio Superti-Furga, Daniel Lackner, Tabea Wiedmer, Alvaro Ingles-Prieto, Barbara Barbosa, Enrico Girardi, Ulrich Goldmann, Bettina Gürtl, Kristaps Klavins, Christoph Klimek, Sabrina Lindinger, Eva Liñeiro-Retes, André C. Müller, Svenja Onstein, Gregor Redinger, Daniela Reil, Vitaly Sedlyarov, Gernot Wolf, Matthew Crawford, Robert Everley, David Hepworth, Shenping Liu, Stephen Noell, Mary Pio Publicado en: Nature Reviews Drug Discovery, Issue 19/7, 2020, Page(s) 429-430, ISSN 1474-1776 Editor: Nature Publishing Group DOI: 10.1038/d41573-020-00056-6 Buscando datos de OpenAIRE... Se ha producido un error en la búsqueda de datos de OpenAIRE No hay resultados disponibles
