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Hybrid Nanostructured Oligonucleotide Platforms for Biomedical Applications

Deliverables

School on synthesis and characterization of hybrid materials for medical applications

The organization of a school on the synthesis and characterization of hybrid materials for medical applications will allow to share the know-how relative to the project among consortium partners, as well as serving as a meeting to strengthen teamwork and build new collaborations.

Protocols for the characterization of the behavior of hybrid supramolecular nanostructures

Protocols should push for high quality data, ensure that experimental results can be confidently shared among research partners and allow meaningful comparison with data reported in published material. The characterization of the behavior of hybrid supramolecular nanostructures in solution will rely on an array of techniques including dynamic light scattering, small-angle X-ray scattering, photophysics, confocal (optical) microscopy. The characterization of the behavior of hybrid supramolecular nanostructures on surfaces will rely on electron microscopies (ESEM, SEM, TEM), liquid drop contact angle, profilometry.

DNA/PNA-BioMSNPs

Production of DNA-BioMSNPs based on Watson and Crick interaction of complementary DNA probes rationally inserted into the programmable SiO2 framework of MSNPs. (M. 24). At least 10 mg of DNA-BioMSNPs based on Watson and Crick interaction of complementary DNA probes rationally inserted into the programmable SiO2 framework of MSNPs.

Porous silicon nanoparticles

Production of porous silicon nanoparticles for the delivery of specific PNAs and DNA therapeutics on a 10mg-scale, by etching of silicon wafers with hydrofluoric acid in ethanol. The pore size of the particles must support the loading of oligonucleotides.

Protocol for the study of stimuli-responsive DNA nanomachines

The protocol should push for high quality data, ensure that experimental results can be confidently shared among research partners and allow meaningful comparison with data reported in published material. The characterization of stimuli-responsive DNA nanomachines will rely on an array of techniques including fluorescence spectroscopies, microscopies and gel electrophoresis.

Protocol for the synthesis of DNA, its modification and conjugation with FRET pair and small molecules

The protocol should provide high-purity, completely characterized materials and molecules, to ensure that products can be confidently shared among research partners and contribute to the positive outcome of the research effort.

Project website

Creation of a website of the project to announce and disseminate published results to the general public (journal papers, youtube videos, etc.)

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