Deliverables Other (6) School on synthesis and characterization of hybrid materials for medical applications The organization of a school on the synthesis and characterization of hybrid materials for medical applications will allow to share the know-how relative to the project among consortium partners, as well as serving as a meeting to strengthen teamwork and build new collaborations. Protocols for the characterization of the behavior of hybrid supramolecular nanostructures Protocols should push for high quality data, ensure that experimental results can be confidently shared among research partners and allow meaningful comparison with data reported in published material. The characterization of the behavior of hybrid supramolecular nanostructures in solution will rely on an array of techniques including dynamic light scattering, small-angle X-ray scattering, photophysics, confocal (optical) microscopy. The characterization of the behavior of hybrid supramolecular nanostructures on surfaces will rely on electron microscopies (ESEM, SEM, TEM), liquid drop contact angle, profilometry. DNA/PNA-BioMSNPs Production of DNA-BioMSNPs based on Watson and Crick interaction of complementary DNA probes rationally inserted into the programmable SiO2 framework of MSNPs. (M. 24). At least 10 mg of DNA-BioMSNPs based on Watson and Crick interaction of complementary DNA probes rationally inserted into the programmable SiO2 framework of MSNPs. Porous silicon nanoparticles Production of porous silicon nanoparticles for the delivery of specific PNAs and DNA therapeutics on a 10mg-scale, by etching of silicon wafers with hydrofluoric acid in ethanol. The pore size of the particles must support the loading of oligonucleotides. Protocol for the study of stimuli-responsive DNA nanomachines The protocol should push for high quality data, ensure that experimental results can be confidently shared among research partners and allow meaningful comparison with data reported in published material. The characterization of stimuli-responsive DNA nanomachines will rely on an array of techniques including fluorescence spectroscopies, microscopies and gel electrophoresis. Protocol for the synthesis of DNA, its modification and conjugation with FRET pair and small molecules The protocol should provide high-purity, completely characterized materials and molecules, to ensure that products can be confidently shared among research partners and contribute to the positive outcome of the research effort. Websites, patent fillings, videos etc. (1) Project website Creation of a website of the project to announce and disseminate published results to the general public (journal papers, youtube videos, etc.) Publications Peer reviewed articles (13) Programming DNA-Based Systems through Effective Molarity Enforced by Biomolecular Confinement Author(s): Marianna Rossetti; Alessandro Bertucci; Tania Patiño; Lorena Baranda; Alessandro Porchetta Published in: Chemistry - A European Journal, 10, 2020, Page(s) Volume 26, Issue 44, Pages 9826 - 9834, ISSN 0947-6539 Publisher: John Wiley & Sons Ltd. DOI: 10.1002/chem.202001660 Pore-forming toxins from sea anemones: from protein-membrane interaction to its implications for developing biomedical applications Author(s): Alvarez, C., Pazos, F., Soto, C., Laborde, R., Lanio, M.E. Published in: Advances in Biomembranes and Lipid Self-Assembly, 2020, Page(s) Advances in Biomembranes and Lipid Self-Assembly, ISSN 2451-9634 Publisher: 24519634 DOI: 10.1016/bs.abl.2020.02.005 Multifunctional Delivery Systems for Peptide Nucleic Acids Author(s): Stefano Volpi; Umberto Cancelli; Martina Neri; Roberto Corradini Published in: Pharmaceuticals, Vol. 14, 2021, Page(s) 1-31, ISSN 1424-8247 Publisher: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/ph14010014 Tuning the Loading and Release Properties of MicroRNA-Silencing Porous Silicon Nanoparticles by Using Chemically Diverse Peptide Nucleic Acid Payloads. Author(s): Martina Neri; Jinyoung Kang; Jonathan M. Zuidema; Jessica Gasparello; Alessia Finotti; Roberto Gambari; Michael J. Sailor; Alessandro Bertucci; Roberto Corradini Published in: ACS Biomaterials Science and Engineering, 8, 2022, Page(s) ACS Biomaterials Science and Engineering, ISSN 2373-9878 Publisher: American Chemical Society DOI: 10.1021/acsbiomaterials.1c00431 Harnessing Effective Molarity to Design an Electrochemical DNA-based Platform for Clinically Relevant Antibody Detection. Author(s): Marianna Rossetti; Simone Brannetti; Marco Mocenigo; Bruna Marini; Rudy Ippodrino; Alessandro Porchetta Published in: Angewandte Chemie - International Edition, 8, 2020, Page(s) Volume 59, Issue 35, Pages 14973 - 14978, ISSN 1433-7851 Publisher: John Wiley & Sons Ltd. DOI: 10.1002/anie.202005124 Multifunctional Delivery Systems for Peptide Nucleic Acids. Author(s): Stefano Volpi; Umberto Cancelli; Martina Neri; Roberto Corradini Published in: Pharmaceuticals, 5, 2021, Page(s) Pharmaceuticals, ISSN 1424-8247 Publisher: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/ph14010014 Panorama of the intracellular molecular concert orchestrated by actinoporins, pore-forming toxins from sea anemones Author(s): Alvarez C, Soto C, Cabezas S, Alvarado-Mesén J, Laborde R, Pazos F, Ros U, Hernández AM, Lanio ME Published in: Toxins, 2021, Page(s) Volume 13, Issue 8, ISSN 2072-6651 Publisher: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/toxins13080567 Hybrid polymer/porous silicon nanofibers for loading and sustained release of synthetic DNA-based responsive devices Author(s): Zuidema, J. M.; Bertucci, A.; Kang, J.; Sailor, M. J.; Ricci, F. Published in: Nanoscale, 2020, Page(s) Volume 12, Issue 4, Pages 2333 - 2339, ISSN 2040-3364 Publisher: Royal Society of Chemistry DOI: 10.1039/c9nr08474f Porous Silicon Nanoparticles Embedded in Poly(lactic-co-glycolic acid) Nanofiber Scaffolds Deliver Neurotrophic Payloads to Enhance Neuronal Growth Author(s): Zuidema, J. M.; Dumont, C. M.; Wang, J.; Batchelor, W. M.; Lu, Y.-S.; Kang, J.; Bertucci, A.; Ziebarth, N. M.; Shea, L. D.; Sailor, M. J., Published in: Advanced Functional Materials, 2020, Page(s) Volume 30, Issue 25,Volume 30, Issue 25, ISSN 1616-301X Publisher: John Wiley & Sons Ltd. DOI: 10.1002/adfm.202002560 Rekindling RNAi Therapy: Materials Design Requirements for In Vivo siRNA Delivery Author(s): Kim, B.; Park, J.-H.; Sailor, M. J. Published in: Advanced Materials, 2019, Page(s) Volume 31, Issue 49, Article number 1903637, ISSN 0935-9648 Publisher: United Nations Industrial Developement Organization DOI: 10.1002/adma.201903637 Porous Silicon Nanoparticle Delivery of Tandem Peptide Anti-Infectives for the Treatment of Pseudomonas aeruginosa Lung Infections Author(s): Kwon, E. J.; Skalak, M.; Bertucci, A.; Braun, G.; Ricci, F.; Ruoslahti, E.; Sailor, M. J.; Bhatia, S. N. Published in: Advanced Materials, 2017, Page(s) Volume 29, Issue 35, Volume 29, Issue 35, ISSN 0935-9648 Publisher: United Nations Industrial Developement Organization DOI: 10.1002/adma.201701527 Engineering DNA-Grafted Quatsomes as Stable Nucleic Acid-Responsive Fluorescent Nanovesicles Author(s): Marianna Rossetti; Lorenzo Stella; Judit Morla-Folch; Sara Bobone; Ariadna Boloix; Lorena Baranda; Danila Moscone; Mónica Roldán; Jaume Veciana; Miguel F. Segura; Mariana Köber; Nora Ventosa; Alessandro Porchetta Published in: Advanced Functional Materials, 3, 2021, Page(s) Volume 31, Issue 46, Article number 2103511, ISSN 1616-301X Publisher: John Wiley & Sons Ltd. DOI: 10.1002/adfm.202103511 Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters Author(s): Marianna Rossetti; Rosa Merlo; Neda Bagheri; Danila Moscone; Anna Valenti; Aakash Saha; Pablo R Arantes; Rudy Ippodrino; Francesco Ricci; Ida Treglia; Elisabetta Delibato; John van der Oost; Giulia Palermo; Giuseppe Perugino; Alessandro Porchetta Published in: Nucleic Acids Research, 10, 2022, Page(s) Volume 50, Issue 14, Pages 8377 - 8391, ISSN 0305-1048 Publisher: Oxford University Press DOI: 10.1093/nar/gkac578 Searching for OpenAIRE data... There was an error trying to search data from OpenAIRE No results available
